Structural and Functional Analysis of the GADD34:PP1 eIF2α Phosphatase [PDF]
Cell Reports, 2015 The attenuation of protein synthesis via the phosphorylation of eIF2α is a major stress response of all eukaryotic cells. The growth-arrest- and DNA-damage-induced transcript 34 (GADD34) bound to the serine/threonine protein phosphatase 1 (PP1) is the ...
Meng S. Choy +7 more
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Yeast Gcn2 retains activity following humanization of its auto-phosphorylation region. [PDF]
FEBS Open BioUsing Saccharomyces cerevisiae as a model to study Gcn2 activation and regulation is limited by the lack of antibodies detecting phosphorylated Gcn2. To overcome this, we engineered Gcn2‐HsC, a yeast Gcn2 variant recognizable by commercial anti‐human phospho‐GCN2 antibodies.
Anderson RA, Schiemann AH, Sattlegger E.
europepmc +2 more sources
Expression of eIF2α and phospho-eIF2α.
, 2023 Huh7.5 cells were electroporated with mJFH-1 WT and DI mutant C142A, C190A and E191A RNAs, together with an NS5B GND mutant as negative control. TM: tunicamycin positive control. Cells were harvested at 72 hpe and lysed with GLB.
Shucheng Chen (1419898) +1 more
core +1 more source
O-GlcNAcylation of eIF2α regulates the phospho-eIF2α-mediated ER stress response [PDF]
, 2015 O-GlcNAcylation is highly involved in cellular stress responses including the endoplasmic reticulum (ER) stress response. For example, glucosamine-induced flux through the hexosamine biosynthetic pathway can promote ER stress and ER stress inducers can ...
Kim, Han Byeol +7 more
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6-Shogaol induces apoptosis in human hepatocellular carcinoma cells and exhibits anti-tumor activity in vivo through endoplasmic reticulum stress. [PDF]
PLoS ONE, 2012 6-Shogaol is an active compound isolated from Ginger (Zingiber officinale Rosc). In this work, we demonstrated that 6-shogaol induces apoptosis in human hepatocellular carcinoma cells in relation to caspase activation and endoplasmic reticulum (ER ...
Rong Hu +10 more
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PLoS ONE, 2020 The integrated stress response (ISR) is one of the most important cytoprotective mechanisms and is integrated by phosphorylation of the α subunit of eukaryotic translation initiation factor 2 (eIF2α).
Yoshimasa Hamada +8 more
doaj +1 more source
eIF2α phosphorylation controls thermal nociception [PDF]
, 2016 A response to environmental stress is critical to alleviate cellular injury and maintain cellular homeostasis. Eukaryotic initiation factor 2 (eIF2) is a key integrator of cellular stress responses and an important regulator of mRNA translation.
Ribeiro-da-Silva, Alfredo +15 more
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Viruses, 2019 Sindbis virus (SINV) infection induces eIF2α phosphorylation, which leads to stress granule (SG) assembly. SINV infection also stimulates autophagy, which has an important role in controlling the innate immune response.
Matthew Jefferson +3 more
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The role of host eIF2α in viral infection
Virology Journal, 2020 Background eIF2α is a regulatory node that controls protein synthesis initiation by its phosphorylation or dephosphorylation. General control nonderepressible-2 (GCN2), protein kinase R-like endoplasmic reticulum kinase (PERK), double-stranded RNA (dsRNA)
Yuanzhi Liu +26 more
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Genetic inhibition of phosphorylation of the translation initiation factor eIF2α does not block Aβ-dependent elevation of BACE1 and APP levels or reduce amyloid pathology in a mouse model of Alzheimer's disease. [PDF]
PLoS ONE, 2014 β-site amyloid precursor protein (APP) cleaving enzyme 1 (BACE1) initiates the production of β-amyloid (Aβ), the major constituent of amyloid plaques in Alzheimer's disease (AD).
Katherine R Sadleir +4 more
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