Results 101 to 110 of about 3,117 (204)

Human Enteroids as a Model of Upper Small Intestinal Ion Transport Physiology and Pathophysiology

, 2016
Background & Aims Human intestinal crypt-derived enteroids are a model of intestinal ion transport that require validation by comparison with cell culture and animal models.
Donowitz, M. (Mark), Foulke-Abel, J, Yin, J. (Jianyi), Kovbasnjuk, O. (Olga), In, J, In, J. (Julie), Estes, MK, Jonge, H.R. (Hugo) de, Estes, M.K. (Mary K.), Kovbasnjuk, O, Foulke-Abel, J. (Jennifer), Zachos, NC, Yin, JY, Donowitz, M, Zachos, N.C. (Nicholas C.), de Jonge, Hugo   +15 more
core   +1 more source

IFN-Lambda 3 Mediates Antiviral Protection Against Porcine Epidemic Diarrhea Virus by Inducing a Distinct Antiviral Transcript Profile in Porcine Intestinal Epithelia

Frontiers in Immunology, 2019
Type III interferon-lambda (IFN-λ) plays a critical role against infection, particularly in mucosal infection in the respiratory and gastrointestinal tract.
Liang Li, Mei Xue, Fang Fu, Lingdan Yin, Li Feng, Pinghuang Liu   +5 more
doaj   +1 more source

Comparison of Surgical and Cadaveric Intestine as a Source of Crypt Culture in Humans

Cell Transplantation, 2020
Human small intestinal crypts are the source of intestinal stem cells (ISCs) that are capable of undergoing self-renewal and differentiation to an epithelial layer.
Andrew Scott, Barbara Olack, Joshua D. Rouch, Hassan A. Khalil, Brent A. Kokubun, Nan Ye Lei, Jiafang Wang, Sergio Solorzano, Michael Lewis, James C.Y. Dunn, Matthias G. Stelzner, Joyce C. Niland, Martín G. Martín   +12 more
doaj   +1 more source

Differential role of M cells in enteroid infection by Mycobacterium avium subsp. paratuberculosis and Salmonella enterica serovar Typhimurium

Frontiers in Cellular and Infection Microbiology
Infection of ruminants such as cattle with Mycobacterium avium subsp. paratuberculosis (MAP) causes Johne’s disease, a disease characterized by chronic inflammation of the small intestine and diarrhoea.
Omar A. Alfituri, Rosemary Blake, Kirsty Jensen, Neil A. Mabbott, Jayne Hope, Joanne M. Stevens   +5 more
doaj   +1 more source

Lgr5+ intestinal stem cell sorting and organoid culture

Animal Models and Experimental Medicine, 2019
Intestinal epithelial stem cells (IESCs) are one of the most rapidly self‐renewing and proliferating adult stem cells. The IESCs reside at the bottom of intestinal and colonic crypts, giving rise to all intestinal epithelial lineages and maintaining ...
Ruixue Liu, Haifeng Li, Juan Cai, Qiang Wei, Xiaonan Han   +4 more
doaj   +1 more source

Human Intestinal Enteroids Model MHC-II in the Gut Epithelium

Frontiers in Immunology, 2019
The role of intestinal epithelial cells (IECs) in mucosal tolerance and immunity remains poorly understood. We present a method for inducing MHC class II (MHC-II) in human enteroids, “mini-guts” derived from small intestinal crypt stem cells, and show ...
Jonathan E. Wosen, Alexandra Ilstad-Minnihan, Julia Y. Co, Wei Jiang, Dhriti Mukhopadhyay, Nielsen Q. Fernandez-Becker, Calvin J. Kuo, Manuel R. Amieva, Manuel R. Amieva, Elizabeth D. Mellins   +9 more
doaj   +1 more source

Adenovirus Infection of Human Enteroids Reveals Interferon Sensitivity and Preferential Infection of Goblet Cells

, 2018
Human adenoviruses (HAdV) are significant human pathogens. Although only a subset of HAdV serotypes commonly cause gastroenteritis in humans, most HAdV species replicate in the gastrointestinal tract.
Mayumi K. Holly, Jason G. Smith
core   +1 more source

CRL inhibition reduces β-TrCP degradation by RV Wa infection in human intestinal enteroids.

, 2016
(A) Brightfield images of human enteroids cultured in 3D Matrigel matrix. Scale bar, 100 μm. (B) Enteroids were infected with human RV Wa strain (MOI = 3) for 16 hr and analyzed by confocal microscopy for viral antigen VP6 (green), cytoskeleton ...
Siyuan Ding (506428), Bin Li (39349), Nancie Mooney (3193788), Peter K. Jackson (211122), Alexander V. Loktev (3193791), Harry B. Greenberg (95102), Ningguo Feng (2630593), Marcus R. Kelly (3193794), John T. Patton (258145), Adrish Sen (287451)   +9 more
core   +1 more source

2′-Fucosyllactose inhibits human norovirus replication in human intestinal enteroids

Journal of Virology
Human noroviruses (HuNoVs) are the leading cause of acute gastroenteritis worldwide. Currently, there are no targeted antivirals for the treatment of HuNoV infection. Histo-blood group antigens (HBGAs) on the intestinal epithelium are cellular attachment
Ketki Patil, B. Vijayalakshmi Ayyar, Nicole M. Hayes, Frederick H. Neill, Lars Bode, Mary K. Estes, Robert L. Atmar, Sasirekha Ramani   +7 more
doaj   +1 more source

Time-Restricted Feeding Reinforces Gut Rhythmicity by Restoring Rhythms in Intestinal Metabolism in a Jetlag Mouse ModelSummary

Cellular and Molecular Gastroenterology and Hepatology
Background & Aims: Circadian disturbances result in adverse health effects, including gastrointestinal symptoms. We investigated which physiological pathways in jejunal mucosa were disrupted during chronic jetlag and prevented during time-restricted ...
Hui Leng, Theo Thijs, Louis Desmet, Guillaume Vanotti, Mona Farhadipour, Inge Depoortere   +5 more
doaj   +1 more source