Suppression of ERK signalling promotes pluripotent epiblast in the human blastocyst [PDF]
Nature CommunicationsStudies in the mouse demonstrate the importance of fibroblast growth factor (FGF) and extra-cellular receptor tyrosine kinase (ERK) in specification of embryo-fated epiblast and yolk-sac-fated hypoblast cells from uncommitted inner cell mass (ICM) cells ...
Claire S. Simon +21 more
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Live imaging and functional characterization of the avian hypoblast redefine the mechanisms of primitive streak induction [PDF]
Nature CommunicationsIn birds and mammals, the formation of the primitive streak, the hallmark of the primary axis and site of gastrulation, is thought to occur when the anterior displacement of the hypoblast (visceral endoderm in mice) lifts its inhibition on NODAL ...
Aurélien Villedieu +5 more
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Stem Cell Research, 2021 The tyrosine kinase Src is highly expressed in embryonic stem cells (ESCs) and ESC-differentiated cells, however, its functional role remains obscured. Here, we constitutively expressed Src in mouse ESCs and found these cells retained comparable levels ...
Juan Luo, Hailin Zou, Peng Li
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Tracing and Capturing the Epiblast Pluripotency of Sheep Preimplantation Embryos [PDF]
Advanced ScienceCapturing different pluripotent state stem cells from epiblast in vitro helps understand embryonic development and provides invaluable cell sources for basic research and regenerative medicine.
Jinying Zhang +28 more
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Large‐Scale Bioprinting of Human Epiblast‐Like Models Featuring Disc‐Shaped Morphogenesis and Gastrulation Events [PDF]
Advanced ScienceUnderstanding the initial weeks of human development remains challenging due to ethical concerns and the restricted availability of human embryos.
Yixue Luo +11 more
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Inverse agonism of retinoic acid receptors directs epiblast cells into the paraxial mesoderm lineage
Stem Cell Research, 2018 We have investigated the differentiation of paraxial mesoderm from mouse embryonic stem cells utilizing a Tbx6-EYFP/Brachyury (T)-Cherry dual reporter system.
Ryan P. Russell +3 more
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The potent neuroepithelium-promoting activity of Otx2 during gastrulation, as demonstrated by its exogenous epiblast-wide expression in chicken embryos [PDF]
Frontiers in Cell and Developmental BiologyAlthough Otx2 has been a topic of research for over 3 decades, the role of Otx2 expressed in the brain-forming anterior epiblast during gastrulation has not been clarified.
Naoya Takami +6 more
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The number of primitive endoderm cells in the inner cell mass is regulated by platelet-derived growth factor signaling in porcine preimplantation embryos [PDF]
Animal Bioscience, 2023 Objective Discovering the mechanism of cell specification is important to manipulate cellular lineages. To obtain lineage-specific cell lines, the target lineage needs to be promoted, and counterpart lineages should be suppressed.
Jong-Nam Oh +7 more
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The Long Terminal Repeats of ERV6 Are Activated in Pre-Implantation Embryos of Cynomolgus Monkey
Cells, 2021 Precise gene regulation is critical during embryo development. Long terminal repeat elements (LTRs) of endogenous retroviruses (ERVs) are dynamically expressed in blastocysts of mammalian embryos.
Kui Duan +8 more
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Capturing Pluripotency and Beyond
Cells, 2021 During the development of a multicellular organism, the specification of different cell lineages originates in a small group of pluripotent cells, the epiblasts, formed in the preimplantation embryo.
Chih-Yu Yeh +3 more
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