An in vitro stem cell model of human epiblast and yolk sac interaction
eLife, 2021 Human embryogenesis entails complex signalling interactions between embryonic and extra-embryonic cells. However, how extra-embryonic cells direct morphogenesis within the human embryo remains largely unknown due to a lack of relevant stem cell models ...
K. Mackinlay +10 more
semanticscholar +3 more sources
NANOG initiates epiblast fate through the coordination of pluripotency genes expression
Nature Communications, 2022 The epiblast is the source of all mammalian embryonic tissues and of pluripotent embryonic stem cells. It differentiates alongside the primitive endoderm in a “salt and pepper” pattern from inner cell mass (ICM) progenitors during the preimplantation ...
N. Allègre +9 more
semanticscholar +2 more sources
The potent neuroepithelium-promoting activity of Otx2 during gastrulation, as demonstrated by its exogenous epiblast-wide expression in chicken embryos [PDF]
Frontiers in Cell and Developmental BiologyAlthough Otx2 has been a topic of research for over 3 decades, the role of Otx2 expressed in the brain-forming anterior epiblast during gastrulation has not been clarified.
Naoya Takami +6 more
doaj +2 more sources
Evolving Lessons on Metazoan Primordial Germ Cells in Diversity and Development [PDF]
Molecular Reproduction and Development, Volume 92, Issue 5, May 2025.ABSTRACT Germ cells are pivotal for the continuation of biological species. The metazoan germline develops from primordial germ cells (PGCs) that undergo multiple rounds of mitotic divisions. The PGCs are specified by either maternal inheritance of asymmetrically polarized cytoplasmic mRNAs/proteins (found in roundworms, flies, fishes, frogs, and fowl)
Indrashis Bhattacharya +5 more
wiley +2 more sources
Non-apical mitoses contribute to cell delamination during mouse gastrulation [PDF]
Nature CommunicationsDuring the epithelial-mesenchymal transition driving mouse embryo gastrulation, cells divide more frequently at the primitive streak, and half of those divisions happen away from the apical pole.
Evangéline Despin-Guitard +10 more
doaj +2 more sources
Morphometric‐Assisted Prediction of Developmental Toxicity Using Stem Cell‐Based Embryo Models in Microwells [PDF]
Advanced Healthcare Materials, Volume 14, Issue 15, 10 June, 2025.This proof‐of‐concept study involves high‐throughput teratogenicity screening of compounds using XEn/EpiCs, a 3D stem cell‐based embryo model, within microwells. The term ‘morphotoxicity’ is introduced to complement traditional cytotoxicity assays through automated feature extraction and machine‐learning‐assisted classification of morphologies.
Vinidhra Shankar +4 more
wiley +2 more sources
New findings on the orientation of the mouse anterior-posterior (A-P) axis before and during the initiation of gastrulation using a more refined embryo staging [PDF]
Biochemistry and Biophysics ReportsA clinically significant event of early mammalian embryogenesis is the generation and early development of the anterior-posterior (A-P) axis, the imaginary line along which the structures from head to tail will form.
Xenia Hadjikypri +3 more
doaj +2 more sources
Primitive to visceral endoderm maturation is essential for mouse epiblast survival beyond implantation [PDF]
iScienceSummary: The implantation of the mouse blastocyst initiates a complex sequence of tissue remodeling and cell differentiation events required for morphogenesis, during which the extraembryonic primitive endoderm transitions into the visceral endoderm ...
Antonia Weberling +5 more
doaj +2 more sources
The number of primitive endoderm cells in the inner cell mass is regulated by platelet-derived growth factor signaling in porcine preimplantation embryos [PDF]
Animal Bioscience, 2023 Objective Discovering the mechanism of cell specification is important to manipulate cellular lineages. To obtain lineage-specific cell lines, the target lineage needs to be promoted, and counterpart lineages should be suppressed.
Jong-Nam Oh +7 more
doaj +1 more source
The Long Terminal Repeats of ERV6 Are Activated in Pre-Implantation Embryos of Cynomolgus Monkey
Cells, 2021 Precise gene regulation is critical during embryo development. Long terminal repeat elements (LTRs) of endogenous retroviruses (ERVs) are dynamically expressed in blastocysts of mammalian embryos.
Kui Duan +8 more
doaj +1 more source