Results 71 to 80 of about 10,397 (178)

Epiblast grafting and in vitro embryo culture

Protocol Exchange, 2015
This protocol describes a method to graft conventional primed pluripotent stem cells (PSCs) and region-selective PSCs to different locations of post-implantation mouse epiblast followed by in vitro embryo culture. Compared to conventionally used teratoma formation assay, epiblast grafting offers a more relevant in vivo test for evaluating the primed ...
Jun Wu, Jun Wu, Daiji Okamura, Juan Carlos Izpisua Belmonte   +3 more
openaire   +2 more sources

In Vivo Differentiation Potential of Epiblast Stem Cells Revealed by Chimeric Embryo Formation

Cell Reports, 2012
Chimera formation after blastocyst injection or morula aggregation is the principal functional assay of the developmental potential of mouse embryonic stem cells (ESCs).
Yali Huang, Rodrigo Osorno, Anestis Tsakiridis, Valerie Wilson   +3 more
doaj   +1 more source

Reprogramming of rabbit induced pluripotent stem cells toward epiblast and chimeric competency using Krüppel-like factors

Stem Cell Research, 2017
Rabbit induced pluripotent stem cells (rbiPSCs) possess the characteristic features of primed pluripotency as defined in rodents and primates. In the present study, we reprogrammed rbiPSCs using human Krüppel-like factors (KLFs) 2 and 4 and cultured them
Yann Tapponnier, Marielle Afanassieff, Irène Aksoy, Maxime Aubry, Anaïs Moulin, Lucas Medjani, Wilhelm Bouchereau, Chloé Mayère, Pierre Osteil, Jazmine Nurse-Francis, Ioannis Oikonomakos, Thierry Joly, Luc Jouneau, Catherine Archilla, Barbara Schmaltz-Panneau, Nathalie Peynot, Harmonie Barasc, Alain Pinton, Jérome Lecardonnel, Elen Gocza, Nathalie Beaujean, Véronique Duranthon, Pierre Savatier   +22 more
doaj   +1 more source

The epigenetic modifier Fam208a is required to maintain epiblast cell fitness

Scientific Reports, 2017
Gastrulation initiates with the formation of the primitive streak, during which, cells of the epiblast delaminate to form the mesoderm and definitive endoderm.
Shohag Bhargava, Brian Cox, Christiana Polydorou, Veronika Gresakova, Vladimir Korinek, Hynek Strnad, Radislav Sedlacek, Trevor Allan Epp, Kallayanee Chawengsaksophak   +8 more
doaj   +1 more source

PDGFRα+ Cells in Embryonic Stem Cell Cultures Represent the In Vitro Equivalent of the Pre-implantation Primitive Endoderm Precursors

Stem Cell Reports, 2017
In early mouse pre-implantation development, primitive endoderm (PrE) precursors are platelet-derived growth factor receptor alpha (PDGFRα) positive. Here, we demonstrated that cultured mouse embryonic stem cells (mESCs) express PDGFRα heterogeneously ...
Antonio Lo Nigro, Anchel de Jaime-Soguero, Rita Khoueiry, Dong Seong Cho, Giorgia Maria Ferlazzo, Ilaria Perini, Vanesa Abon Escalona, Xabier Lopez Aranguren, Susana M. Chuva de Sousa Lopes, Kian Peng Koh, Pier Giulio Conaldi, Wei-Shou Hu, An Zwijsen, Frederic Lluis, Catherine M. Verfaillie   +14 more
doaj   +1 more source

Pluripotency Potential of Embryonic Stem Cell-Like Cells Derived from Mouse Testis

Cell Journal, 2019
Objective: During the cultivation of spermatogonial stem cells (SSCs) and their conversion into embryonic stem-like (ES-like) cells, transitional ES-like colonies and epiblast-like cells were observable.
Hossein Azizi, Behruz Asgari, Thomas Skutella   +2 more
doaj   +1 more source

Single-Cell RNA-Seq Reveals Cellular Heterogeneity of Pluripotency Transition and X Chromosome Dynamics during Early Mouse Development

Cell Reports, 2019
Summary: Following implantation, the epiblast (EPI) cells transit from the naive to primed pluripotency, accompanied by dynamic changes in X chromosome activity in females. To investigate the molecular attributes of this process, we performed single-cell
Shangli Cheng, Yu Pei, Liqun He, Guangdun Peng, Björn Reinius, Patrick P.L. Tam, Naihe Jing, Qiaolin Deng   +7 more
doaj   +1 more source

ZMYM2 inhibits NANOG-mediated reprogramming [version 1; peer review: 2 approved]

Wellcome Open Research, 2019
Background: NANOG is a homeodomain-containing transcription factor which forms one of the hubs in the pluripotency network and plays a key role in the reprogramming of somatic cells and epiblast stem cells to naïve pluripotency.
Moyra Lawrence, Thorold W. Theunissen, Patrick Lombard, David J. Adams, José C. R. Silva   +4 more
doaj   +1 more source

p53-mediated regulation of epiblast cell numbers predicts reactivation during mouse embryonic diapause

Cell Reports
Summary: Embryonic diapause is a temporary suspension of proliferation in mammalian pre-implantation embryos, allowing for reactivation later. Cells in mouse diapause embryos enter the G0 phase within 7 days of diapause initiation.
Megumi Murata, Akihito Harada, Haruka Hirose, Hinako Maeda, Teppei Shimamura, Yasuyuki Ohkawa, Tatsuya Takemoto, Katsuyoshi Takaoka   +7 more
doaj   +1 more source

Primitive to visceral endoderm maturation is essential for mouse epiblast survival beyond implantation

iScience
Summary: The implantation of the mouse blastocyst initiates a complex sequence of tissue remodeling and cell differentiation events required for morphogenesis, during which the extraembryonic primitive endoderm transitions into the visceral endoderm ...
Antonia Weberling, Dylan Siriwardena, Christopher Penfold, Neophytos Christodoulou, Thorsten E. Boroviak, Magdalena Zernicka-Goetz   +5 more
doaj   +1 more source