Results 31 to 40 of about 1,604 (142)

A Multiplexed Single-Cell CRISPR Screening Platform Enables Systematic Dissection of the Unfolded Protein Response [PDF]

, 2018
Functional genomics efforts face tradeoffs between number of perturbations examined and complexity of phenotypes measured. We bridge this gap with Perturb-seq, which combines droplet-based single-cell RNA-seq with a strategy for barcoding CRISPR-mediated
Adamson, Britt, Chen, Yuwen, Cho, Min Y., Dixit, Atray C., Gilbert, Luke A., Gray, Andrew N., Gross, Carol A., Hein, Marco Y., Horlbeck, Max A., Jost, Marco, Norman, Thomas M., Nuñez, James K., Pak, Ryan A., Parnas, Oren, Regev, Aviv, Villalta, Jacqueline E., Weissman, Jonathan S.   +16 more
core   +1 more source

ATF4 links ER stress with reticulophagy in glioblastoma cells [PDF]

, 2020
Selective degradation of the endoplasmic reticulum (ER; reticulophagy) is a type of autophagy involved in the removal of ER fragments. So far, amino acid starvation as well as ER stress have been described as inducers of reticulophagy, which in turn ...
Covarrubias-Pinto, Adriana, Fulda, Simone, Kardo, Simon, Kinzler, Maximilian N, Kögel, Donat, Mari, Muriel, Meyer, Nina, Reggiori, Fulvio, Stolz, Alexandra, van Wijk, Sjoerd J L, Zein, Laura, Zielke, Svenja   +11 more
core   +1 more source

Endoplasmic reticulum–nuclei signaling enzyme-1 knockdown modulates effect of hypoxia and ischemia on the expression of circadian genes in glioma cells

Біологічні студії, 2011
The main molecular components of circadian clock system are proteins which play a significant role in the control of both metabolism and malignant tumor growth.
L. L. Karbovskyi, D. O. Minchenko, S. V. Danylovsky, M. Moenner, O. H. Minchenko   +4 more
doaj   +1 more source

The GST-BHMT assay reveals a distinct mechanism underlying proteasome inhibition-induced macroautophagy in mammalian cells [PDF]

, 2015
By monitoring the fragmentation of a GST-BHMT (a protein fusion of glutathionine S-transferase N-terminal to betaine-homocysteine S-methyltransferase) reporter in lysosomes, the GST-BHMT assay has previously been established as an endpoint, cargo-based ...
Sheng Zhang, Yan-Ning Rui, Zhen Xu, Zhihua Chen   +3 more
core   +1 more source

IRE1-XBP1 Pathway of the Unfolded Protein Response Is Required during Early Differentiation of C2C12 Myoblasts [PDF]

, 2019
ArticleInternational Journal of Molecular Sciences. 21(1): 182.
Arai, Wataru, Harada, Kiyonori, Hayashi, Satoko, Tokutake, Yukako, Watanabe, Takafumi, Yamada, Keita, Yonekura, Shinichi   +6 more
core   +1 more source

ER stress in obesity pathogenesis and management. [PDF]

, 2021
Given the unprecedented global pandemic of obesity, a better understanding of the etiology of adiposity will be necessary to ensure effective management of obesity and related complications.
Ajoolabady, Amir, Kavalakatt, Sina, Klionsky, Daniel, Lip, Gregory, Liu, Simin, Pereira, David, Ren, Jun, Samali, Afshin, Tuomilehto, Jaakko   +8 more
core   +1 more source

Glucose deprivation affects the expression of genes encoding cAMP-activated protein kinase and related proteins in U87 glioma cells in ERN1 dependent manner

Endocrine Regulations, 2020
Objective. The aim of this investigation was to study the expression of genes encoding cAMP-activated protein kinase catalytic and regulatory A subunits (PRKACA and PRKAR1A) and related proteins such as cAMP-dependent protein kinase inhibitors A and G ...
Ratushna Oksana O.
doaj   +1 more source

Inhibition of ERN1 signaling enzyme affects hypoxic regulation of the expression of E2F8, EPAS1, HOXC6, ATF3, TBX3 and FOXF1 genes in U87 glioma cells [PDF]

The Ukrainian Biochemical Journal, 2015
Hypoxia as well as the endoplasmic reticulum stress are important factors of malignant tumor growth and control of the expression of genes, which regulate numerous metabolic processes and cell proliferation.
O. H. Minchenko, D. O. Tsymbal, D. O. Minchenko, O. V. Kovalevska, L. L. Karbovskyi, A. Bikfalvi   +5 more
doaj   +1 more source

Metabolic synthetic lethality by targeting NOP56 and mTOR in KRAS-mutant lung cancer. [PDF]

, 2022
BACKGROUND Oncogenic KRAS mutations are prevalent in human cancers, but effective treatment of KRAS-mutant malignancies remains a major challenge in the clinic.
Chen, Chun, Dorn, Patrick, Gao, Yanyun, Hegedüs, Balazs, Kocher, Gregor J, Marti, Thomas M, Peng, Ren-Wang, Schmid, Ralph A, Shun-Qing, Liang, Wang, Wenxiang, Yang, Haitang, Yang, Zhang, Zhao, Liang, Zheng, Bin   +13 more
core   +1 more source

Effect of hypoxia and glutamine or glucose deprivation on the expression of retinoblastoma and retinoblastoma-related genes in ERN1 knockdown glioma U87 cell line

American Journal of Molecular Biology, 2012
The expression of retinoblastoma and several retinoblastoma-related genes was studied in glioma cell line U87 and its subline with knockdown of ERN1 (endoplasmic reticulum—nuclei-1), the main endoplasmic reticulum stress sensing and signaling enzyme.
Dmytro O. Minchenko, Leonid L. Karbovskyi, Serhii V. Danilovskyi, Michel Moenner, Oleksandr H. Minchenko   +4 more
openaire   +2 more sources