Separation of Fucosyltransferase and Endogenous Acceptors of Fucose from Human Parotid Saliva [PDF]
, 1977 Using gel filtration on Sephadex G-100 and column chromatography on CM-cellulose, fractions containing either endogenous acceptor of L-fucose or fucosyltransferase were obtained from human parotid saliva.
Iwasaki, E. +11 more
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Diagnosis and prognosis of serum Fut8 for epilepsy and refractory epilepsy in children
PLoS ONE, 2023 With adequate serum concentration of antiepileptic drugs, the epilepsy symptoms in many patients still cannot be controlled well. The alteration of glycosyltransferase has obvious influence on the pathogenesis of epilepsy.
Yunxiu Huang, Zhou Zhang, Linmu Chen
doaj +2 more sources
Scientific Reports, 2023 Glycosylation of proteins and lipids in viruses and their host cells is important for viral infection and is a target for antiviral therapy. Hepatitis B virus (HBV) is a major pathogen that causes acute and chronic hepatitis; it cannot be cured because ...
Tsunenori Ouchida +10 more
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Microbiota Depletion Promotes Human Rotavirus Replication in an Adult Mouse Model
Biomedicines, 2021 Intestinal microbiota-virus-host interaction has emerged as a key factor in mediating enteric virus pathogenicity. With the aim of analyzing whether human gut bacteria improve the inefficient replication of human rotavirus in mice, we performed fecal ...
Roberto Gozalbo-Rovira +8 more
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Clinical Medicine Insights: Oncology, 2023 Background: The incidence of intrahepatic cholangiocarcinoma (ICC) is increasing globally, and its prognosis has not improved substantially in recent years. Understanding the pathogenesis of ICC may provide a theoretical basis for its treatment.
Jingheng Guo +7 more
doaj +1 more source
The one-pot multienzyme (OPME) synthesis of human blood group H antigens and a human milk oligosaccharide (HMOS) with highly active Thermosynechococcus elongatus α1–2-fucosyltransferase [PDF]
, 2016 A novel α1-2-fucosyltransferase from Thermosynechococcus elongatus BP-1 (Te2FT) with high fucosyltransferase activity and low donor hydrolysis activity was discovered and characterized.
Liu, Bin +8 more
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Genetic engineering strategies to prevent the effects of antibody and complement on xenogeneic chondrocytes [PDF]
European Cells & Materials, 2015 Advances in animal transgenesis may allow using xenogeneic chondrocytes in tissue-engineering applications for clinical cartilage repair. Porcine cartilage is rejected by humoral and cellular mechanisms that could be overcome by identifying key molecules
R Sommaggio +5 more
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Determination of α1–6-fucosyltransferase activity.
, 2014 Recombinant FLAG-tagged Sf9 FUT8 was produced using the baculovirus-Sf9 cell system and purified using anti-FLAG M2 affinity gel. The fucosyltransferase activity of the recombinant protein was tested with GDP-[14C]-L-fucose, as substrate, after two days (
François Monjaret (647590) +7 more
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Enzymatic Synthesis of a Fluorogenic Reporter Substrate and a High-Throughput Assay for Fucosyltransferase VIII Provide a Toolkit to Probe and Inhibit Core Fucosylation [PDF]
, 2019 We report a straight-forward enzymatic synthesis of the 4-methylumbelliferyl glycoside of a complex-type oligosaccharide substrate for core-fucosylation.
David, Kwan +3 more
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Subcellular localisation of cerebral fucosyltransferase
, 1984 GDP-fucose : asialofetuin fucosyltransferase from sheep brain was fractionated on a sucrose gradient into two activity peaks. Using purification on Ficoll adapted from the proposed method [(1980) J. Neurochem.
Pierre Louisot +9 more
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