Results 91 to 100 of about 1,908 (177)
EGFP-G3BP1 overexpressing cells form As-induced SGs and do not form SGs spontaneously.
, 2022 Immunofluorescence microscopy analysis of 293A[EGFP] and 293A[EGFP-G3BP1] cells untreated (-) or treated with arsenite (+ As) and stained for G3BP2 (magenta). GFP signal is shown in teal. Scale bar = 50 μm. (DOCX)
Shan Ying (13822804) +5 more
core +1 more source
G3BP1, G3BP2 and CAPRIN1 regulate establishment of the antiviral state.
, 2014 HuH-7 cells treated with siGFP, siG12C#1 or siG12C#2 were stimulated with the indicated concentration of IFN-β for 16 h and ISG mRNA and protein levels were determined.
Mariano A. Garcia-Blanco (136317) +2 more
core +1 more source
Nsp1 inhibits eIF2α phosphorylation and SG formation.
, 2022 (A) 293A cells were transiently transfected with the indicated HA-tagged Nsp1 expression constructs or EGFP control. At 24 h post-transfection cells were treated with sodium arsenite (+ As) and SG formation analysed by immunofluorescence microscopy ...
Shan Ying (13822804) +5 more
core +1 more source
Molecular surface mimicry enables CRBN to target G3BP2 for degradation
Molecular glue degraders (MGDs) are small molecule compounds that repurpose the ubiquitin-proteasome system to induce degradation of challenging therapeutic targets. Clinically effective MGDs bind cereblon (CRBN), a substrate receptor of the Cullin-4/RING E3 ubiquitin ligase (CRL4CRBN), and impose a gain-of-function activity to recruit, ubiquitinate ...
Stefano Annunziato +32 more
openaire +1 more source
G3BP1, G3BP2 and CAPRIN1 are required for IFN-β mediated antiviral activity against DENV-2.
, 2014 (A) HuH-7 cells were treated with siGFP or one of two independent sets of siRNAs (siG12C#1 and siG12C#2) targeting G3BP1, G3BP2 and CAPRIN1, and knockdown was confirmed by western blot analysis.
Mariano A. Garcia-Blanco (136317) +2 more
core +1 more source
Correlation of transcriptome profile with electrical activity in temporal lobe epilepsy
Neurobiology of Disease, 2006 The biology underlying epileptic brain activity in humans is not well understood and likely depends on changes in gene expression. We performed a microarray transcriptome profiling of 12 anterolateral temporal cortical samples originating from five ...
Dominique Arion +9 more
doaj +1 more source
, 2023 Mutagenesis of the serine rich region (SR) of SARS-CoV-2 N alters the cellular distribution of N but does not affect its ability to inhibit ISR-induced translation arrest. HeLa R19 cells were transfected to express SARS-CoV-2 N (13S>A)-EGFP.
Jelle G. Schipper (16866034) +7 more
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G3BP1, G3BP2 and CAPRIN1 are required for association of ISG mRNAs with polyribosomes.
, 2014 (A) HuH-7 cells treated with control siGFP or two independent sets of siRNAs against G3BP1, G3BP2 and CAPRIN1 and treated or not with 100 UI/ml IFN-β for 4 h were labeled with 35S metabolic for 1 h. Cell lysates were separated on a 4–15% SDS-PAGE gel and
Mariano A. Garcia-Blanco (136317) +2 more
core +1 more source
G3BP1, G3BP2 and CAPRIN1 interact with DENV-2 gRNA and sfRNA in infected cells.
, 2014 (A) The DENV-2 NGC 3′UTR variable region (VR) is predicted to contain five stem-loops (SL-I to SL-V) and two highly conserved pseudoknots PKSL-II and PKSL-IV. Sequences shared by DENV-2 gRNA and sfRNA are highlighted in grey. To relatively quantify these
Mariano A. Garcia-Blanco (136317) +2 more
core +1 more source
Cell ReportsSummary: Although the composition and assembly of stress granules (SGs) are well understood, the molecular mechanisms underlying SG disassembly remain unclear. Here, we identify that heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNPA2B1) is associated
Xiaoli Wang +11 more
doaj +1 more source