Results 181 to 190 of about 87,868 (301)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Connexin-43 in Cancer: Above and Beyond Gap Junctions! [PDF]

Cancers (Basel)
Paunikar S, Tamagnone L.
europepmc   +1 more source

Gap junctions and hemichannels keep the RPE connected. [PDF]

J Gen Physiol, 2022
Short B.
europepmc   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Connexin 36-mediated gap junctions contribute to fine odour discrimination and late-phase excitation of mitral cells in the mouse olfactory bulb. [PDF]

J Physiol
Kuruppath P, Jones ST, Pouille F, Ramirez-Gordillo D, Restrepo D, Schoppa NE.   +5 more
europepmc   +1 more source

Connexins, Pannexins and Gap Junctions in Perinatal Brain Injury. [PDF]

Biomedicines, 2022
McDouall A, Zhou KQ, Bennet L, Green CR, Gunn AJ, Davidson JO.   +5 more
europepmc   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Domain-like patterning of dendritic asymmetry and orientation defined by gap junctions in the mouse retina. [PDF]

iScience
Szarka G, Futácsi A, Kovács-Öller T, Balogh B, Czéh B, Völgyi B.   +5 more
europepmc   +1 more source

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

FEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source

The Role of Gap Junctions in MSC-EA.hy926 (An Endothelial Cell Model) Crosstalk Under Hypoxic Stress: Regulation of the Angiogenic Response. [PDF]

Int J Mol Sci
Ezdakova M, Matveeva D, Lobanova M.
europepmc   +1 more source