Results 31 to 40 of about 698,006 (308)

Variable episomal silencing of a recombinant herpesvirus renders its encoded GFP an unreliable marker of infection in primary cells. [PDF]

PLoS ONE, 2014
The availability of reliable recombinant reporter virus systems has been a great boon to the study of Kaposi's sarcoma-associated herpesvirus (KSHV/HHV-8).
Thomas J Ellison, Dean H Kedes
doaj   +1 more source

Formation of a Unique Cluster of G-Quadruplex Structures in the HIV-1 nef Coding Region: Implications for Antiviral Activity [PDF]

, 2013
G-quadruplexes are tetraplex structures of nucleic acids that can form in G-rich sequences. Their presence and functional role have been established in telomeres, oncogene promoters and coding regions of the human chromosome.
Frasson, Ilaria, Nadai, Matteo, Palumbo, Manlio, Palù, Giorgio, Perrone, Rosalba, Poe, Jerrod A., Qiu, Jianming, Richter, Sara N., Smithgall, Thomas E.   +8 more
core   +2 more sources

Viral Evolution as a Tool to Improve the Tetracycline-regulated Gene Expression System [PDF]

Journal of Biological Chemistry, 2004
We present viral evolution as a novel and powerful method to optimize non-viral proteins. We used this approach to optimize the tetracycline (Tc)-regulated gene expression system (Tet system) for its function in mammalian cells. The components of the Tet system were incorporated in the human immunodeficiency virus (HIV)-1 virus such that viral ...
Das, Atze T., Zhou, Xue, Vink, Monique, Klaver, Bep, Verhoef, Koen, Marzio, Giuseppe, Berkhout, Ben   +6 more
openaire   +2 more sources

Transcriptional Targeting and MicroRNA Regulation of Lentiviral Vectors

Molecular Therapy: Methods & Clinical Development, 2019
Gene expression regulation is the result of complex interactions between transcriptional and post-transcriptional controls, resulting in cell-type-specific gene expression patterns that are determined by the developmental and differentiation stage of ...
Simone Merlin, Antonia Follenzi
doaj   +1 more source

Unfolding SARS-CoV-2 viral genome to understand its gene expression regulation

Infection, Genetics and Evolution, 2020
SARS-CoV-2 is a new virus responsible for an outbreak of respiratory illness known as COVID-19, which has spread to several countries around the world and a global effort is being undertaken to characterize the molecular features and evolutionary origins of this virus. In silico analysis of the transcription start sites, promoter regions, transcription
Dinka, Hunduma, Milkesa, Ashenafi
openaire   +2 more sources

Novel RNA Viral Vectors for Chemically Regulated Gene Expression in Embryonic Stem Cells

ACS Synthetic Biology, 2021
RNA viral vectors that replicate without DNA intermediates are attractive platforms for manipulation of cells for biomedical and veterinary applications because they have minimal risk of chromosomal integration. Vesicular stomatitis virus (VSV) vectors are among the most well-studied RNA viral vectors due to their low pathogenicity to humans and ...
Narae Kim, Yohei Yokobayashi
openaire   +2 more sources

Keratinocyte differentiation-dependent human papillomavirus gene regulation [PDF]

, 2017
Human papillomaviruses (HPVs) cause diseases ranging from benign warts to invasive cancers. HPVs infect epithelial cells and their replication cycle is tightly linked with the differentiation process of the infected keratinocyte.
Graham, Sheila V.
core   +1 more source

Multidimensional analysis of Gammaherpesvirus RNA expression reveals unexpected heterogeneity of gene expression.

PLoS Pathogens, 2019
Virus-host interactions are frequently studied in bulk cell populations, obscuring cell-to-cell variation. Here we investigate endogenous herpesvirus gene expression at the single-cell level, combining a sensitive and robust fluorescent in situ ...
Lauren M Oko, Abigail K Kimball, Rachael E Kaspar, Ashley N Knox, Carrie B Coleman, Rosemary Rochford, Tim Chang, Benjamin Alderete, Linda F van Dyk, Eric T Clambey   +9 more
doaj   +1 more source

Differentially-Expressed Pseudogenes in HIV-1 Infection. [PDF]

, 2015
Not all pseudogenes are transcriptionally silent as previously thought. Pseudogene transcripts, although not translated, contribute to the non-coding RNA pool of the cell that regulates the expression of other genes.
Adami, Christoph, Brown, C Titus, Gupta, Aditi, Zheng, Yong-Hui   +3 more
core   +2 more sources

Reconstruction of the regulatory hypermethylation network controlling hepatocellular carcinoma development during hepatitis C viral infection

Journal of Integrative Bioinformatics, 2023
Hepatocellular carcinoma (HCC) has been associated with hepatitis C viral (HCV) infection as a potential risk factor. Nonetheless, the precise genetic regulatory mechanisms triggered by the virus, leading to virus-induced hepatocarcinogenesis, remain ...
Antropova Evgeniya A., Khlebodarova Tamara M., Demenkov Pavel S., Volianskaia Anastasiia R., Venzel Artur S., Ivanisenko Nikita V., Gavrilenko Alexandr D., Ivanisenko Timofey V., Adamovskaya Anna V., Revva Polina M., Kolchanov Nikolay A., Lavrik Inna N., Ivanisenko Vladimir A.   +12 more
doaj   +1 more source