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Integration of Organoids With CRISPR Screens: A Narrative Review. [PDF]
Biol CellMukhare R +8 more
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RNAi Screening in Tumor Cells Identifies Artificial microRNAs That Improve Oncolytic Virus Replication. [PDF]
Pharmaceuticals (Basel)Klemets H +9 more
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TnSeq identifies genetic requirements of Mycobacterium tuberculosis for survival under vaccine-induced immunity. [PDF]
NPJ VaccinesJames KS +7 more
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Fantastic genes and where to find them expressed in CHO. [PDF]
Comput Struct Biotechnol JRiedl M, Ruggeri C, Marx N, Borth N.
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Gene discovery by ribozyme and siRNA libraries
Nature Reviews Molecular Cell Biology, 2005Catalytic RNAs, also known as ribozymes, can be engineered to optimize their activities in the intracellular environment. The introduction of a library of active ribozymes into cells, and the subsequent screening for phenotypic changes, allows the rapid identification of gene function.
Sahohime Matsumoto +3 more
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Construction of gene libraries for each human chromosome [PDF]
Cytometry, 1990 AbstractWe describe the construction of two complete sets of small insert, complete digest DNA libraries for each of the 24 human chromosomal types by the National Laboratory Gene Library Project. Flow sorting was used to purify the chromosomes which provided the DNA for cloning.
Marvin A. Van Dilla, Larry L. Deaven
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Stalking the Gene -- DNA Libraries
New England Journal of Medicine, 1994For molecular biologists in search of clues to cell function, the ultimate object of inquiry is the gene. A scientist looking for a particular gene faces a formidable challenge, because each cell contains more than 100,000 different genes scattered over billions of nucleotides.
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