A Synthetic Gene Library Yields a Previously Unknown Glycoside Phosphorylase That Degrades and Assembles Poly-β-1,3-GlcNAc, Completing the Suite of β-Linked GlcNAc Polysaccharides. [PDF]
ACS Cent Sci, 2022 Macdonald SS +9 more
europepmc +1 more source
Identification of a CD4+ T cell-stimulating antigen of pathogenic bacteria by expression cloning. [PDF]
, 1995 Identifying the immunogenic proteins that elicit pathogen-specific T cell responses is key to rational vaccine design. While several approaches have succeeded in identifying major histocompatibility complex (MHC) class I bound peptides that stimulate CD8+
Campbell, D +2 more
core +1 more source
Rapid creation and quantitative monitoring of high coverage shRNA libraries. [PDF]
, 2009 Short hairpin RNA libraries are limited by low efficacy of many shRNAs and by off-target effects, which give rise to false negatives and false positives, respectively. Here we present a strategy for rapidly creating expanded shRNA pools (approximately 30
Bassik, Michael C +8 more
core +2 more sources
Meiotic cDNA libraries reveal gene truncations and mitochondrial proteins important for competitive fitness in Saccharomyces cerevisiae [PDF]
, 2022 Tina L. Sing +9 more
openalex +1 more source
Identification of putative dothistromin biosynthetic genes : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Molecular Biology at Massey University, Palmerston North, New Zealand [PDF]
, 1998 Dothistromin is a polyketide-derived mycotoxin produced by the Pinus pathogen Dothistroma pini, and is thought to be important in the development of the necrotic disease Dothistroma needle blight.
Monahan, Brendon Joseph
core
Creating New β-Globin-Expressing Lentiviral Vectors by High-Resolution Mapping of Locus Control Region Enhancer Sequences. [PDF]
, 2020 Hematopoietic stem cell gene therapy is a promising approach for treating disorders of the hematopoietic system. Identifying combinations of cis-regulatory elements that do not impede packaging or transduction efficiency when included in lentiviral ...
Aleshe, Bamidele +14 more
core +1 more source
Stem Cell Reports, 2017 Summary: Sickle cell anemia affects millions of people worldwide and is an emerging global health burden. As part of a large NIH-funded NextGen Consortium, we generated a diverse, comprehensive, and fully characterized library of sickle-cell-disease ...
Seonmi Park +18 more
doaj +1 more source
Faculty recital series: John Muratore, guitar with Roberto Cassan, accordion, November 8, 2007 [PDF]
, 2007 This is the concert program of the Faculty recital series: John Muratore, guitar with Roberto Cassan, accordion performance on Thursday, November 8, 2007 at 8:00 p.m., at the Tsai Performance Center, 685 Commonwealth Avenue.
School of Music, Boston University
core
GOGrapher: A Python library for GO graph representation and analysis
BMC Research Notes, 2009 Background The Gene Ontology is the most commonly used controlled vocabulary for annotating proteins. The concepts in the ontology are organized as a directed acyclic graph, in which a node corresponds to a biological concept and a directed edge denotes ...
Lu Xinghua +3 more
doaj +1 more source
Molecular Systems Biology, 2018 High‐content imaging using automated microscopy and computer vision allows multivariate profiling of single‐cell phenotypes. Here, we present methods for the application of the CISPR‐Cas9 system in large‐scale, image‐based, gene perturbation experiments.
Reinoud de Groot +4 more
doaj +1 more source