Results 11 to 20 of about 5,248,714 (172)

Genetic characterization of human immunodeficiency virus type 1 nef gene products translated in vitro and expressed in mammalian cells [PDF]

Journal of Virology, 1991
Expression of the human immunodeficiency virus type 1 nef gene was studied by in vitro transcription-translation and by transfection into monkey COS cells. Two Nef-related peptides, of 27 and 25 kDa, were identified by immunoprecipitation with anti-Nef antibodies.
J, Kaminchik, N, Bashan, A, Itach, N, Sarver, M, Gorecki, A, Panet   +5 more
openaire   +3 more sources

Functional association between the nef gene product and gag‐pol region of HIV‐1 [PDF]

FEBS Letters, 2000
Nef gene function is diverse among virus isolates of primate immunodeficiency viruses. We found differential effects of nef mutation on the virus replication between two HIV‐1 clones, NL432 and LAI. The nef mutation in NL432 affected the infectivity more severely compared with that in LAI, although the Nef functions of both clones were comparable ...
Ono, Takeshi, Iwatani, Yasumasa, Nishimura, Akiko, Ishimoto, Akinori, Sakai, Hiroyuki   +4 more
openaire   +3 more sources

Effects of the tat and nef gene products of human immunodeficiency virus type 1 (HIV-1) on transcription controlled by the HIV-1 long terminal repeat and on cell growth in macrophages [PDF]

Journal of Virology, 1993
The RAW264 murine macrophage cell line was used as a model to examine the role of the tat and nef gene products in the transcription regulation of the human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR) in macrophages. Contrary to claims that the activity of the HIV-1 LTR responds poorly in rodent cells to trans activation by the ...
Murphy, Kathleen M., Sweet, Matthew J., Ross, Ian L., Hume, David A.   +3 more
core   +10 more sources

Species-specific activity of SIV Nef and HIV-1 Vpu in overcoming restriction by tetherin/BST2. [PDF]

PLoS Pathogens, 2009
Tetherin, also known as BST2, CD317 or HM1.24, was recently identified as an interferon-inducible host-cell factor that interferes with the detachment of virus particles from infected cells.
Bin Jia, Ruth Serra-Moreno, William Neidermyer, Andrew Rahmberg, John Mackey, Ismael Ben Fofana, Welkin E Johnson, Susan Westmoreland, David T Evans   +8 more
doaj   +2 more sources

Formation of a Unique Cluster of G-Quadruplex Structures in the HIV-1 nef Coding Region: Implications for Antiviral Activity [PDF]

, 2013
G-quadruplexes are tetraplex structures of nucleic acids that can form in G-rich sequences. Their presence and functional role have been established in telomeres, oncogene promoters and coding regions of the human chromosome.
NADAI, MATTEO, PALU', GIORGIO, Qiu, Jianming, Smithgall, TE, Jerrod A Poe, J. A. Poe, Palumbo, M, Matteo Nadai, Ilaria Frasson, Poe, Jerrod A., Giorgio Palù, RICHTER, SARA, T. E. Smithgall, Poe, JA, Frasson Ilaria, Poe Jerrod A., Palù, Giorgio, Smithgall, Thomas E., Sara N Richter, Rosalba Perrone, Nadai, M, Palumbo Manlio, Richter, Sara N., Manlio Palumbo, Richter Sara N., Palù, G, Perrone, R, Richter, SN, Smithgall Thomas E., Palù Giorgio, Thomas E Smithgall, PERRONE, ROSALBA, Frasson, I, Nadai Matteo, FRASSON, ILARIA, PALUMBO, MANLIO, Perrone Rosalba   +36 more
core   +2 more sources

HIV-specific T cell responses reflect substantive in vivo interactions with antigen despite long-term therapy

JCI Insight, 2021
Antiretroviral therapies (ARTs) abrogate HIV replication; however, infection persists as long-lived reservoirs of infected cells with integrated proviruses, which reseed replication if ART is interrupted.
Eva M. Stevenson, Adam R. Ward, Ronald Truong, Allison S. Thomas, Szu-Han Huang, Thomas R. Dilling, Sandra Terry, John K. Bui, Talia M. Mota, Ali Danesh, Guinevere Q. Lee, Andrea Gramatica, Pragya Khadka, Winiffer D. Conce Alberto, Rajesh T. Gandhi, Deborah K. McMahon, Christina M. Lalama, Ronald J. Bosch, Bernard Macatangay, Joshua C. Cyktor, Joseph J. Eron, John W. Mellors, R. Brad Jones, for the AIDS Clinical Trials Group A5321 Team   +23 more
doaj   +1 more source

Expression, intracellular targeting and purification of HIV Nef variants in tobacco cells [PDF]

, 2007
Background Plants may represent excellent alternatives to classical heterologous protein expression systems, especially for the production of biopharmaceuticals and vaccine components.
Buriani, Giampaolo, Nuttall, James, Marusic Carla, Lombardi Raffaele, Lombardi, Raffaele, Baschieri , Selene, Buriani Giampaolo, Eugenio Benvenuto, Lico Chiara, Baschieri Selene, Frigerio, Lorenzo, James Nuttall, Benvenuto Eugenio, Lorenzo Frigerio, Nuttall James, Giampaolo Buriani, Marusic, Carla, Selene Baschieri, Frigerio Lorenzo, Raffaele Lombardi, Benvenuto, Eugenio, Chiara Lico, Carla Marusic, Lico, Chiara   +23 more
core   +1 more source

Polymorphism of the Human Immunodeficiency Virus Type 1 in Brazil: Genetic Characterization of the nef Gene and Implications for Vaccine Design

Memorias do Instituto Oswaldo Cruz, 2002
Most of the Brazilian HIV-1 samples have been characterized based on the structural genes (env, gag and pol) and no data concerning the variability of the accessory genes such as nef have been available so far.
ML Guimarães, AS Moreira, MG Morgado
doaj   +1 more source

Development of a Novel Q-body Using an In Vivo Site-Specific Unnatural Amino Acid Incorporation System

Sensors, 2018
A Q-body capable of detecting target molecules in solutions could serve as a simple molecular detection tool. The position of the fluorescent dye in a Q-body affects sensitivity and therefore must be optimized.
Yoichi Kurumida, Nobuhiro Hayashi
doaj   +1 more source

Glomerulosclerosis and viral gene expression in HIV-transgenic mice: Role of nef [PDF]

, 2000
Glomerulosclerosis and viral gene expression in HIV-transgenic mice: Role of nef.BackgroundHuman immunodeficiency virus (HIV)-associated nephropathy is characterized by focal segmental glomerulosclerosis and microcystic tubular dilation.
Marinos, Nancy J., Dickie, Peter, Klotman, Paul E., Kajiyama, Wataru, Kopp, Jeffrey B.   +4 more
core   +1 more source