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Genotyping by Sequencing Advancements in Barley [PDF]

open access: yesFrontiers in Plant Science, 2022
Barley is considered an ideal crop to study cereal genetics due to its close relationship with wheat and diploid ancestral genome. It plays a crucial role in reducing risks to global food security posed by climate change. Genetic variations in the traits
Nirmal Raj Rajendran   +2 more
doaj   +5 more sources

Genotyping-by-Sequencing in Plants

open access: yesBiology, 2012
The advent of next-generation DNA sequencing (NGS) technologies has led to the development of rapid genome-wide Single Nucleotide Polymorphism (SNP) detection applications in various plant species.
Gregory D. May   +2 more
doaj   +3 more sources

GBS-DP: a bioinformatics pipeline for processing data coming from genotyping by sequencing [PDF]

open access: yesВавиловский журнал генетики и селекции, 2023
The development of next-generation sequencing technologies has provided new opportunities for genotyping various organisms, including plants. Genotyping by sequencing (GBS) is used to identify genetic variability more rapidly, and is more cost-effective ...
A. Y. Pronozin   +2 more
doaj   +2 more sources

Genotyping 1000 yeast strains by next-generation sequencing [PDF]

open access: yesBMC Genomics, 2013
Background The throughput of next-generation sequencing machines has increased dramatically over the last few years; yet the cost and time for library preparation have not changed proportionally, thus representing the main bottleneck for sequencing large
Wilkening Stefan   +8 more
doaj   +4 more sources

Adapting Genotyping-by-Sequencing for Rice F2 Populations

open access: yesG3: Genes, Genomes, Genetics, 2017
Rapid and cost-effective genotyping of large mapping populations can be achieved by sequencing a reduced representation of the genome of every individual in a given population, and using that information to generate genetic markers.
Tomoyuki Furuta   +4 more
doaj   +3 more sources

Improving genomic predictions by correction of genotypes from genotyping by sequencing in livestock populations [PDF]

open access: yesJournal of Animal Science and Biotechnology, 2019
Background Genotyping by sequencing (GBS) is a robust method to genotype markers. Many factors can influence the genotyping quality. One is that heterozygous genotypes could be wrongly genotyped as homozygotes, dependent on the genotyping depths. In this
Xiao Wang   +5 more
doaj   +5 more sources

Genotyping-by-Sequencing for Plant Breeding and Genetics

open access: yesThe Plant Genome, 2012
Rapid advances in “next-generation” DNA sequencing technology have brought the US$1000 human () genome within reach while providing the raw sequencing output for researchers to revolutionize the way populations are genotyped.
Jesse A. Poland, Trevor W. Rife
doaj   +2 more sources

Single Nucleotide Polymorphism (SNP) Discovery and Association Study of Flowering Times, Crude Fat and Fatty Acid Composition in Rapeseed (Brassica napus L.) Mutant Lines Using Genotyping-by-Sequencing (GBS)

open access: yesAgronomy, 2021
Rapeseed is the most important oil crop used in the food and biodiesel industries. In this study, based on single nucleotide polymorphism (SNP) identified from genotyping-by-sequencing (GBS), and an association study of flowering time, crude fat and ...
Jaihyunk Ryu   +11 more
doaj   +1 more source

tGBS® genotyping-by-sequencing enables reliable genotyping of heterozygous loci [PDF]

open access: yesNucleic Acids Research, 2017
Conventional genotyping-by-sequencing (cGBS) strategies suffer from high rates of missing data and genotyping errors, particularly at heterozygous sites. tGBS® genotyping-by-sequencing is a novel method of genome reduction that employs two restriction enzymes to generate overhangs in opposite orientations to which (single-strand) oligos rather than ...
Ott, Alina   +5 more
openaire   +3 more sources

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