Optimizing ddRADseq in Non-Model Species: A Case Study in Eucalyptus dunnii Maiden
Restriction site-associated DNA sequencing (RADseq) and its derived protocols, such as double digest RADseq (ddRADseq), offer a flexible and highly cost-effective strategy for efficient plant genome sampling.
Natalia Cristina Aguirre +14 more
doaj +1 more source
Discovery and genotyping of novel sequence insertions in many sequenced individuals [PDF]
Abstract Motivation Despite recent advances in algorithms design to characterize structural variation using high-throughput short read sequencing (HTS) data, characterization of novel sequence insertions longer than the average read length remains a challenging task.
Pinar Kavak +6 more
openaire +5 more sources
A single-nucleotide-polymorphism real-time PCR assay for genotyping of mycobacterium tuberculosis complex in peri-urban Kampala [PDF]
Accurate and high-throughput genotyping of Mycobacterium tuberculosis complex (MTBC) may be important for understanding the epidemiology and pathogenesis of tuberculosis (TB).
Eddie M. Wampande +32 more
core +2 more sources
Genomic Prediction in Tetraploid Ryegrass Using Allele Frequencies Based on Genotyping by Sequencing
Perennial ryegrass is an outbreeding forage species and is one of the most widely used forage grasses in temperate regions. The aim of this study was to investigate the possibility of implementing genomic prediction in tetraploid perennial ryegrass, to ...
Xiangyu Guo +7 more
doaj +1 more source
tGBS® genotyping-by-sequencing enables reliable genotyping of heterozygous loci [PDF]
Conventional genotyping-by-sequencing (cGBS) strategies suffer from high rates of missing data and genotyping errors, particularly at heterozygous sites. tGBS® genotyping-by-sequencing is a novel method of genome reduction that employs two restriction enzymes to generate overhangs in opposite orientations to which (single-strand) oligos rather than ...
Ott, Alina +5 more
openaire +2 more sources
Distribution of genotype network sizes in sequence-to-structure genotype–phenotype maps [PDF]
An essential quantity to ensure evolvability of populations is the navigability of the genotype space. Navigability, understood as the ease with which alternative phenotypes are reached, relies on the existence of sufficiently large and mutually attainable genotype networks. The size of genotype networks (e.g. the number of RNA sequences folding into a
Susanna Manrubia, José A. Cuesta
openaire +3 more sources
An Integrated Genotyping‐by‐Sequencing Polymorphism Map for Over 10,000 Sorghum Genotypes [PDF]
Mining crop genomic variation can facilitate the genetic research of complex traits and molecular breeding. In sorghum [ Sorghum bicolor L. (Moench)], several large‐scale single nucleotide polymorphism (SNP) datasets have been generated using ...
Zhenbin Hu +3 more
openaire +3 more sources
cnvCapSeq: detecting copy number variation in long-range targeted resequencing data. [PDF]
Targeted resequencing technologies have allowed for efficient and cost-effective detection of genomic variants in specific regions of interest. Although capture sequencing has been primarily used for investigating single nucleotide variants and indels ...
Bellos, Evangelos; https://orcid.org/ +42 more
core +1 more source
The domestic rabbit (Oryctolagus cuniculus f. domesticus) is a very important variety in biomedical research and agricultural animal breeding.
Congyan Li +15 more
doaj +1 more source
Deciphering the genetic basis underlying agronomic traits is of importance for soybean improvement. However, covariation, modulated by genetic correlations between complicated traits via hub QTLs, commonly affects the efficiency and accuracy of soybean ...
Mengmeng Fu +8 more
doaj +1 more source

