Results 251 to 260 of about 289,333 (295)

Genotyping‐by‐Sequencing

Current Protocols in Plant Biology, 2017
AbstractGenotyping‐by‐sequencing (GBS) refers to a suite of related methods that obtain genotype data from samples by using restriction enzyme digestion followed by high‐throughput sequencing. GBS is a refinement of restriction site–associated DNA sequencing (RADseq) methods, with a goal of being able to perform library preparations quickly, cost ...
Jason G, Wallace, Sharon E, Mitchell
openaire   +2 more sources

Skim-Based Genotyping by Sequencing

2014
Genotyping by sequencing (GBS) is a relatively new method used to determine the differences in the genetic makeup of individuals. Its novelty stems from a combination of two already available methods: genotyping and next-generation sequencing. Depending on the individual study design GBS protocols can take multiple forms, however most share a sequence ...
Golicz, Agnieszka A.   +2 more
openaire   +3 more sources

Methods for Genotyping-by-Sequencing

2016
A major goal for biologists is to understand the connection between genes and phenotypic traits, and genetic mapping in experimental populations remains a powerful approach for discovering the causal genes underlying phenotypes. For genetic mapping, the process of genotyping was previously a major rate-limiting step.
Rowan, B.   +4 more
openaire   +3 more sources

Genotyping Mycoplasma gallisepticum by multilocus sequence typing

Veterinary Microbiology, 2019
Mycoplasma gallisepticum causes chronic respiratory disease and reproductive disorders in many bird species, resulting in considerable economic losses to the poultry industry. Maintenance of M. gallisepticum-free flocks is the most adequate method to control infection.
Bekő, Katinka   +11 more
openaire   +3 more sources

Genotyping by Multiplexed Sequencing (GMS) Using SNP Markers

2023
SNP-based genotyping has become the most effective approach to generate target-specific data for use in genetic studies. In this chapter, we will describe a high-throughput genotyping method that multiplexes hundreds to thousands of SNP markers in a two-step PCR protocol that can be customized to fit the specific needs of a study.
Travis M, Ruff   +4 more
openaire   +2 more sources

Multiplexed ISSR Genotyping by Sequencing (MIG-Seq)

2023
Multiplexed inter-simple sequence repeat (ISSR) genotyping by sequencing (MIG-seq) is a simple, rapid, and inexpensive method for detecting single-nucleotide polymorphisms (SNPs) using next-generation sequencing (NGS). The advantages of MIG-seq include easy application to various species without prior genetic information. In addition, this method opens
openaire   +2 more sources

Genotyping-by-Sequencing Technology in Plant Taxonomy and Phylogeny [PDF]

open access: possible, 2020
Genotyping-by-sequencing (GBS) is a method to discover and genotype simultaneous genome-wide high-throughput single nucleotide polymorphisms (SNPs). GBS is based on reducing genome complexity with restriction enzymes. Here we describe a method developed by Elshire et al.
Favre, Félicien   +3 more
openaire   +3 more sources

Genotyping-by-Sequencing in Potato

2017
Genotyping-by-sequencing (GBS) is one of the most cost-effective approaches to sequencing in potato (SNP) discovery and genotyping. The reduction of genome complexity that is central to the GBS approach is useful in the analysis of many plant genomes in which large size and polyploidy can prove challenging.
Chiheb Boudhrioua   +6 more
openaire   +1 more source

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