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Transcriptome analysis of melatonin regulating the transformation of glucoraphanin to sulforaphane in broccoli hairy roots

Physiology and Molecular Biology of Plants, 2022
Sulforaphane (SF) is one of the most effective natural products in preventing and fighting cancer, found in cruciferous plants. In this study, broccoli hairy roots grown for 20 d were used as the experimental material, and it was treated with 500 μmol/L melatonin (MT) for 0, 12 and 32 h to explore the effect of MT on the conversion of glucoraphanin ...
Xiaoling Zhang   +7 more
openaire   +2 more sources

Formation of glucoraphanin by chemoselective oxidation of natural glucoerucin: A chemoenzymatic route to sulforaphane

Bioorganic & Medicinal Chemistry Letters, 1999
A new semi-synthetic way to produce glucoraphanin (2), the bio-precursor of the potential anticarcinogen sulforaphane (3), has been developed. Starting from glucoerucin (1), isolated from ripe seeds of Eruca sativa, glucoraphanin was obtained through chemoselective oxidation.
R, Iori   +4 more
openaire   +2 more sources

Engineering of methionine chain elongation part of glucoraphanin pathway in E. coli

Metabolic Engineering, 2016
The methionine-derived glucosinolate glucoraphanin is associated with the health-promoting properties of broccoli. This has developed a strong interest in producing this compound in high amounts from a microbial source. Glucoraphanin synthesis starts with a five-gene chain elongation pathway that converts methionine to dihomo-methionine, which is ...
Mirza, Nadia Muhammad Akram   +3 more
openaire   +3 more sources

Quantitative determination of glucoraphanin in Brassica vegetables by micellar electrokinetic capillary chromatography

Analytica Chimica Acta, 2010
Glucoraphanin, a glucosinolate, is found naturally in plants and is present in relatively high concentrations in broccoli. Glucosinolates have received much attention as studies have indicated that a diet rich in them may provide some protection from certain cancers.
Iris, Lee   +2 more
openaire   +2 more sources

Glucoraphanin and sulforaphane evolution during juice preparation from broccoli sprouts

Food Chemistry, 2018
Broccoli sprouts are considered functional food as they are naturally enriched in glucoraphanin (GR) that is the biological precursor of the anticancer compound sulforaphane (SFN). Due to its health promoting value, also broccoli sprout juice is becoming very popular.
Cristiano Bello   +4 more
openaire   +2 more sources

Collard landraces are novel sources of glucoraphanin and other aliphatic glucosinolates

Plant Breeding, 2015
AbstractThe glucosinolate make‐up of the edible parts of some Brassica oleracea L. crops has been investigated previously, but the leafy‐green collard (B. oleracea var. viridis) remains relatively unstudied on this topic. Due to this lack of information, a collection of US landraces was examined for glucosinolate content of leaves.
Zachary Stansell   +3 more
openaire   +1 more source

Optimization of Heterologous Glucoraphanin Production In Planta

ACS Synthetic Biology, 2022
Patrick M Shih
exaly  

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