A Microfluidic Platform for Actin‐Based Membrane Remodeling Reveals the Stabilizing Role of Branched Actin Networks on Lipid Microdomains [PDF]
Small ScienceCell shape changes, essential for processes such as motility or division, are controlled by the actomyosin cortex that actively remodels biological membranes.
Lixin Huang +9 more
doaj +2 more sources
Mechanisms of Self-Assembly of Giant Unilamellar Vesicles in the Army Liposome Formulation (ALF) Family of Vaccine Adjuvants [PDF]
PharmaceuticsBackground/Objectives: Army Liposome Formulation with QS21 (ALFQ) is a vaccine adjuvant formulation consisting of liposomes that contain saturated zwitterionic and anionic phospholipids, 55 mol% cholesterol, and small molar amounts of monophosphoryl ...
Calin Nicolescu +4 more
doaj +2 more sources
Dimensions, stability, and deformability of DOPC-cholesterol giant unilamellar vesicles formed by droplet transfer [version 3; peer review: 2 approved, 2 approved with reservations] [PDF]
Open Research EuropeBackground Understanding cell membrane-like lipid bilayers is crucial for studying fundamental biological mechanisms. Giant Unilamellar Vesicles (GUVs) are key tools for this investigation and have applications in both synthetic biology and, more ...
Stefano Palagi +3 more
doaj +2 more sources
Exploring the dynamics of a single vesicle induced by Fe₃O₄ nanoparticles using micropipette manipulation. [PDF]
PLoS ONEMagnetite nanoparticles (MNPs, Fe3O4) have gained substantial interest for different biomedical and biochemical applications. Therefore, it is important to understand the mechanism of interaction between MNPs and cell membranes.
Nazia Ahmed +2 more
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PLoS ONE, 2021 A new purification technique is developed for obtaining distribution of giant unilamellar vesicles (GUVs) within a specific range of sizes using dual filtration. The GUVs were prepared using well known natural swelling method.
Mohammad Abu Sayem Karal +7 more
doaj +1 more source
Formation of Giant Unilamellar Vesicles Assisted by Fluorinated Nanoparticles
Advanced Science, 2023 In the quest to produce artificial cells, one key challenge that remains to be solved is the recreation of a complex cellular membrane. Among the existing models, giant unilamellar vesicles (GUVs) are particularly interesting due to their intrinsic ...
Jorik Waeterschoot +5 more
doaj +1 more source
Whole-GUV patch-clamping [PDF]
Proceedings of the National Academy of Sciences, 2016 Significance Although membrane composition and tension modulate the activity of ion channels and transporters, this protein–membrane coupling has been challenging to study due to the difficulty of controlling membrane properties in cells and technical limitations of existing in vitro systems.
Garten, Matthias +5 more
openaire +3 more sources
Imaging of photoacoustic-mediated permeabilization of giant unilamellar vesicles (GUVs)
Scientific Reports, 2021 Target delivery of large foreign materials to cells requires transient permeabilization of the cell membrane without toxicity. Giant unilamellar vesicles (GUVs) mimic the phospholipid bilayer of the cell membrane and are also useful drug delivery ...
Diogo A. Pereira +9 more
doaj +1 more source
Methods to mechanically perturb and characterize GUV-based minimal cell models
Computational and Structural Biotechnology Journal, 2023 Cells shield organelles and the cytosol via an active boundary predominantly made of phospholipids and membrane proteins, yet allowing communication between the intracellular and extracellular environment.
Nadab H. Wubshet, Allen P. Liu
doaj +1 more source
PLoS ONE, 2022 Sugar plays a vital role in the structural and functional characteristics of cells. Hence, the interaction of NPs with cell membranes in the presence of sugar concentrations is important for medicinal and pharmacological innovations.
Sharif Hasan +5 more
doaj +2 more sources