Results 11 to 20 of about 12,147 (240)

Generation of Giant Unilamellar Vesicles (GUVs) Using Polyacrylamide Gels

Bio-Protocol, 2020
Giant unilamellar vesicles (GUVs) are a widely used model system for a range of applications including membrane biophysics, drug delivery, and the study of actin dynamics.
Eric Parigoris, Daniel Dunkelmann, Unai Silvan   +2 more
doaj   +2 more sources

Microfluidic production, stability and loading of synthetic giant unilamellar vesicles

Scientific Reports
In advanced drug delivery, versatile liposomal formulations are commonly employed for safer and more accurate therapies. Here we report a method that allows a straightforward production of synthetic monodisperse (~ 100 μm) giant unilamellar vesicles ...
Mart Ernits, Olavi Reinsalu, Naresh Yandrapalli, Sergei Kopanchuk, Ehsan Moradpur-Tari, Immanuel Sanka, Ott Scheler, Ago Rinken, Reet Kurg, Andreas Kyritsakis, Veikko Linko, Veronika Zadin   +11 more
doaj   +2 more sources

Rapid Multi-Well Evaluation of Assorted Materials for Hydrogel-Assisted Giant Unilamellar Vesicle Production: Empowering Bottom-Up Synthetic Biology

Gels
Giant unilamellar vesicles (GUVs) are versatile cell models in biomedical and environmental research. Of the various GUV production methods, hydrogel-assisted GUV production is most easily implemented in a typical biological laboratory. To date, agarose,
Cherng-Wen Darren Tan, Magdalena Schöller, Eva-Kathrin Ehmoser   +2 more
doaj   +2 more sources

An integrated microfluidic platform to fabricate single-micrometer asymmetric giant unilamellar vesicles (GUVs) using dielectrophoretic separation of microemulsions.

Biomicrofluidics, 2021
We present a microfluidic technique that generates asymmetric giant unilamellar vesicles (GUVs) in the size range of 2-14 μm. In our method, we (i) create water-in-oil emulsions as the precursors to build synthetic vesicles, (ii) deflect the emulsions ...
Sepehr Maktabi, N. Malmstadt, Jeffrey W. Schertzer, Paul R. Chiarot   +3 more
semanticscholar   +1 more source

Use of giant unilamellar lipid vesicles as antioxidant carriers in in vitro culture medium of bovine embryos

Scientific Reports, 2022
Giant unilamellar vesicles (GUVs) are composed of lipophilic layers and are sensitive to the action of reactive oxygen species (ROS). The use of GUVs as microcarriers of biological macromolecules is particularly interesting since ROS produced by gametes ...
Luana Teixeira Rodrigues Rossi, Giovana Barros Nunes, Cíntia Rodrigues da Silva, Hugo de Rossi, Priscila Helena dos Santos, Marcelo Fábio Gouveia Nogueira, Pedro Henrique Benites Aoki, Gisele Zoccal Mingoti   +7 more
doaj   +1 more source

Functional reconstitution of a voltage-gated potassium channel in giant unilamellar vesicles. [PDF]

PLoS ONE, 2011
Voltage-gated ion channels are key players in cellular excitability. Recent studies suggest that their behavior can depend strongly on the membrane lipid composition and physical state. In vivo studies of membrane/channel and channel/channel interactions
Sophie Aimon, John Manzi, Daniel Schmidt, Jose Antonio Poveda Larrosa, Patricia Bassereau, Gilman E S Toombes   +5 more
doaj   +1 more source

Caspase-8 binding to cardiolipin in giant unilamellar vesicles provides a functional docking platform for bid [PDF]

, 2013
Caspase-8 is involved in death receptor-mediated apoptosis in type II cells, the proapoptotic programme of which is triggered by truncated Bid. Indeed, caspase-8 and Bid are the known intermediates of this signalling pathway.
AH Stegh, AH Stegh, AJ Garcia-Saez, AJ Garcia-Saez, AM Chinnaiyan, Ana-Jesus García-Sáez, B Apellaniz, B Leber, Beate Klösgen, Boris Zhivotovsky, C Scaffidi, D Ardail, D Ardail, D Chandra, D Marsh, D Marsh, D Needham, DW Chang, E Evans, E Krieghoff, E van den Brink-van der Laan, EE Varfolomeev, Eyal Gottlieb, F Gonzalvez, F Gonzalvez, F Gonzalvez, F Gonzalvez, FC Kischkel, François Gonzalvez, G Kroemer, GC Shearman, H Zhang, HR Stennicke, J Zha, Jesus Ayala-Sanmartin, JF Lovell, JH Li, JP Medema, K Milovic-Holm, KH Lee, KM Boatright, L Mathivet, Liberty François-Moutal, M Lamblet, M Lutter, M Lutter, M Muzio, M Muzio, MA Hughes, Mark Perry, MI Angelova, MO Hengartner, MP Boldin, N Keller, Olivier Jalmar, P Juo, P Ratanabanangkoon, Patrice X. Petit, Petra Schwille, PJ Jost, RF Epand, S Zhang, T Granjon, T Kuwana, T Parasassi, Thierry Granjon, VA Torres, VA Torres, W Helfrich, W Rawicz, WC Yeh, X Luo, Y Zaltsman, ZT Schug   +73 more
core   +11 more sources

Effects of osmotic pressure on the irreversible electroporation in giant lipid vesicles.

PLoS ONE, 2021
Irreversible electroporation (IRE) is a nonthermal tumor/cell ablation technique in which a series of high-voltage short pulses are used. As a new approach, we aimed to investigate the rupture of giant unilamellar vesicles (GUVs) using the IRE technique ...
Malay Kumar Sarkar, Mohammad Abu Sayem Karal, Marzuk Ahmed, Md Kabir Ahamed, Shareef Ahammed, Sabrina Sharmin, Sayed Ul Alam Shibly   +6 more
doaj   +1 more source

QS21-Initiated Fusion of Liposomal Small Unilamellar Vesicles to Form ALFQ Results in Concentration of Most of the Monophosphoryl Lipid A, QS21, and Cholesterol in Giant Unilamellar Vesicles

Pharmaceutics, 2023
Army Liposome Formulation with QS21 (ALFQ), a vaccine adjuvant preparation, comprises liposomes containing saturated phospholipids, with 55 mol% cholesterol relative to the phospholipids, and two adjuvants, monophosphoryl lipid A (MPLA) and QS21 saponin.
Erwin G. Abucayon, Mangala Rao, Gary R. Matyas, Carl R. Alving   +3 more
doaj   +1 more source

Liposome-based liquid handling platform featuring addition, mixing, and aliquoting of femtoliter volumes. [PDF]

PLoS ONE, 2014
This paper describes the utilization of giant unilamellar vesicles (GUVs) as a platform for handling chemical and biochemical reagents. GUVs with diameters of 5 to 10 µm and containing chemical/biochemical reagents together with inert polymers were fused
Hideaki Shiomi, Soichiro Tsuda, Hiroaki Suzuki, Tetsuya Yomo   +3 more
doaj   +1 more source