Results 31 to 40 of about 12,147 (240)

Dewetting-induced formation and mechanical properties of synthetic bacterial outer membrane models (GUVs) with controlled inner-leaflet lipid composition.

open access: yesSoft Matter, 2019
The double-membrane cellular envelope of Gram-negative bacteria enables them to endure harsh environments and represents a barrier to many clinically available antibiotics.
Sepehr Maktabi   +2 more
semanticscholar   +1 more source

A protocol for mimicking lipid-mediated phase separation on the membrane using giant unilamellar vesicles

open access: yesSTAR Protocols, 2022
Summary: Here, we present a general protocol for mimicking lipid-mediated phase separation on the membrane using giant unilamellar vesicles (GUVs). In this protocol, we use GUVs to mimic Ago1 protein’s phase separation behavior on the membrane through ...
Yuanxiang Zhu   +3 more
doaj   +1 more source

ESCRT-III mediated cell division in Sulfolobus acidocaldarius - a reconstitution perspective [PDF]

open access: yes, 2014
In the framework of synthetic biology, it has become an intriguing question what would be the minimal representation of cell division machinery. Thus, it seems appropriate to compare how cell division is realized in different microorganisms. Inparticular,
Härtel, T., Schwille, P.
core   +2 more sources

On-chip inverted emulsion method for fast giant vesicle production, handling, and analysis

open access: yes, 2020
Liposomes and giant unilamellar vesicles (GUVs) in particular are excellent compartments for constructing artificial cells. Traditionally, their use requires bench-top vesicle growth, followed by experimentation under a microscope.
Robinson, T.   +2 more
core   +1 more source

Microparticle assembly pathways on lipid membranes [PDF]

open access: yes, 2017
Understanding interactions between microparticles and lipid membranes is of increasing importance, especially for unraveling the influence of microplastics on our health and environment.
Heinrich, Doris   +2 more
core   +3 more sources

GUV preparation v1

open access: yes, 2022
GUV preparation for membrane tube assay application
Liv Jensen, Chunmei Chang
openaire   +1 more source

Electroformation of Giant Unilamellar Vesicles from Damp Lipid Films Formed by Vesicle Fusion

open access: yesMembranes, 2023
Giant unilamellar vesicles (GUVs) are artificial membrane models which are of special interest to researchers because of their similarity in size to eukaryotic cells. The most commonly used method for GUVs production is electroformation.
Zvonimir Boban   +5 more
doaj   +1 more source

Giant Unilamellar Vesicle Electroformation: What to Use, What to Avoid, and How to Quantify the Results

open access: yesMembranes, 2021
Since its inception more than thirty years ago, electroformation has become the most commonly used method for growing giant unilamellar vesicles (GUVs). Although the method seems quite straightforward at first, researchers must consider the interplay of ...
Zvonimir Boban   +3 more
doaj   +1 more source

Linker length in fluorophore–cholesterol conjugates directs phase selectivity and cellular localisation in GUVs and live cells

open access: yesRSC Advances, 2019
Lipid membrane fluorescent probes that are both domain-selective and compatible with demanding microscopy methods are crucial to elucidate the presence and function of rafts and domains in cells and biophysical models.
Darragh O' Connor   +2 more
semanticscholar   +1 more source

Probing Interactions between AuNPs/AgNPs and Giant Unilamellar Vesicles (GUVs) Using Hyperspectral Dark-field Microscopy

open access: yesInternational Journal of Molecular Sciences, 2018
Noble metallic nanoparticles (NPs) such as gold and silver nanoparticles (AuNPs and AgNPs) have been shown to exhibit anti-tumor effect in anti-angiogenesis, photothermal and radio therapeutics.
A. Bhat   +4 more
semanticscholar   +1 more source

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