Results 41 to 50 of about 34,607 (204)

High CD45 expression of CD8+ and CD4+ T cells correlates with the size of HIV-1 reservoir in blood

Scientific Reports, 2020
Using mass cytometry, we investigated the expression of 28 markers on CD8+ and CD4+ T cells from HIV-1 infected patients with a variable size of HIV-1 reservoir defined as high (HR) and low (LR) reservoir; we aimed at identifying phenotypic associations ...
Stefan Petkov, Yonas Bekele, Tadepally Lakshmikanth, Bo Hejdeman, Maurizio Zazzi, Petter Brodin, Francesca Chiodi   +6 more
doaj   +1 more source

Extensive virologic and immunologic characterization in an HIV-infected individual following allogeneic stem cell transplant and analytic cessation of antiretroviral therapy: A case study. [PDF]

PLoS Medicine, 2017
BACKGROUND:Notwithstanding 1 documented case of HIV-1 cure following allogeneic stem cell transplantation (allo-SCT), several subsequent cases of allo-SCT in HIV-1 positive individuals have failed to cure HIV-1 infection.
Nathan W Cummins, Stacey Rizza, Mark R Litzow, Stephane Hua, Guinevere Q Lee, Kevin Einkauf, Tae-Wook Chun, Frank Rhame, Jason V Baker, Michael P Busch, Nicolas Chomont, Patrick G Dean, Rémi Fromentin, Ashley T Haase, Dylan Hampton, Sheila M Keating, Steven M Lada, Tzong-Hae Lee, Sekar Natesampillai, Douglas D Richman, Timothy W Schacker, Stephen Wietgrefe, Xu G Yu, Joseph D Yao, John Zeuli, Mathias Lichterfeld, Andrew D Badley   +26 more
doaj   +1 more source

Mechanoadaptation via Myosin Cytoplasmic Redistribution Protects Circulating Tumor Cells From Shear‐induced Death During Hematogenous Dissemination

Advanced Science, EarlyView.
This study investigates how CTCs survive varying shear stress during hematogenous metastasis. We uncover a self‐protection mechanism, by which non‐adherent CTCs adapt to high shearing milieu through accumulated cytoplasmic myosin‐mediated disruption of myosin‐actin binding, attenuating force transmission into chromatin to protect CTCs from shear ...
Cunyu Zhang, Qianchun Wang, Keming Li, Guanshuo Hu, Ying Xin, Kai Tang, Bing Hu, Pengyu Du, Renwei Mao, Baohua Ji, Youhua Tan   +10 more
wiley   +1 more source

Endogenous Engineering Reprograms Extracellular Vesicles for Enhanced Therapeutic Function

Advanced Science, EarlyView.
This review explains how Extracellular vesicles‐producing cells can be endogenously engineered to load therapeutic proteins and nucleic acids. We summarize physiological and genetic strategies that harness native sorting pathways for selective cargo loading.
Jinghui Wang, Wenxuan Zhao, Xiaoming Hu, Yishu Wang, Peidong Yang, Diwei Zheng, Yugang Wang, Guanghui Ma, Min Shi, Wei Wei, Shuang Wang   +10 more
wiley   +1 more source

Towards an HIV-1 cure: measuring the latent reservoir [PDF]

Trends in Microbiology, 2015
The latent reservoir (LR) of HIV-1 in resting memory CD4(+) T cells serves as a major barrier to curing HIV-1 infection. While many PCR- and culture-based assays have been used to measure the size of the LR, correlation between results of different assays is poor and recent studies indicate that no available assay provides an accurate measurement of ...
Katherine M, Bruner, Nina N, Hosmane, Robert F, Siliciano   +2 more
openaire   +2 more sources

Development of a Human 3D Immune‐Competent Neurovascular Model Enabling Time‐Resolved Monitoring of Neuroinflammatory Dynamics and Neuroimmune Interactions

Advanced Science, EarlyView.
Neuroinflammation alters blood–brain barrier integrity and contributes to neurological disorders, yet existing models lack human immune complexity. This study presents a 3D, immune‐responsive platform that reconstructs key neurovascular components and enables real‐time monitoring.
Pavlo Gordiichuk, Jing Bai, Olurotimi A. Bolonduro, Andrew M. Silverman, Robert Madison Green, Jason H. Lasser, Paul W. Fleming, Lishomwa C. Ndhlovu, James F. Saunders, Dmitry Shvartsman   +9 more
wiley   +1 more source

Regulatory T cells: the ultimate HIV reservoir?

Journal of Virus Eradication, 2018
Despite significant advances in the understanding of HIV-1 infection, a cure remains out of reach. This is, in part, due to a long-lived HIV-1 reservoir in resting CD4+ T cells, which do not express viral antigens, and thus are invisible to the immune ...
Joseph Rocco, John W. Mellors, Bernard J.C. Macatangay   +2 more
doaj   +1 more source

Redefining the Viral Reservoirs that Prevent HIV-1 Eradication [PDF]

Immunity, 2012
This Perspective proposes definitions for key terms in the field of HIV-1 latency and eradication. In the context of eradication, a reservoir is a cell type that allows persistence of replication-competent HIV-1 on a timescale of years in patients on optimal antiretroviral therapy. Reservoirs act as a barrier to eradication in the patient population in
Eisele, Evelyn, Siliciano, Robert F.
openaire   +2 more sources

Humanized immune system animal models and their recent applications

Animal Models and Experimental Medicine, EarlyView.
In this review, we provide an updated and comprehensive overview of the current state of humanized immune system animal model research. We discuss the variety of techniques used to produce humanized mice with their respective strengths and weaknesses, and evaluate the advantages and limitations of these models, including issues with incomplete immune ...
Nicolas Skuli, Rudra B. Amin, A’ishah Bakayoko, Marisa Kruidenier, Sahara Aqui, Sarah J. Skuli, Terence P. Gade   +6 more
wiley   +1 more source

Nonviral Gold Nanoparticle‐Mediated Delivery of CRISPR‐Cas9 Ribonucleoprotein and Long DNA Transgenes Into Primary Blood Cells

Advanced NanoBiomed Research, EarlyView.
CRISPR/Cas9 has revolutionized the field of gene therapy, but delivery remains an outstanding issue. We propose a nonviral gold‐nanoparticle platform for co‐delivery of CRISPR/Cas9 ribonucleoprotein and long 2.1 kilobase dsDNA transgene constructs. This CRISPR‐AuNP is inexpensive to produce and mediate gene editing and DNA delivery in T cells and CD34+
Rachel A. Cunningham, Karthikeya S. V. Gottimukkala, Daniel D. Lane, Katrina Poljakov, Patricia Lipson, Mark R. Enstrom, Alessandro Rizzi, Aude G. Chapuis, Jennifer E. Adair   +8 more
wiley   +1 more source