Host‐induced gene silencing inhibits the biotrophic pathogen causing downy mildew of lettuce [PDF]
SummaryHost‐induced gene silencing (HIGS) is an RNA interference‐based approach in which small interfering RNAs (siRNAs) are produced in the host plant and subsequently move into the pathogen to silence pathogen genes. As a proof‐of‐concept, we generated stable transgenic lettuce plants expressing siRNAs targeting potentially vital genes of Bremia ...
Govindarajulu, Manjula +3 more
openaire +4 more sources
Internuclear gene silencing in Phytophthora infestans is established through chromatin remodelling [PDF]
In the plant pathogen Phytophthora infestans, nuclear integration of inf1 transgenic DNA sequences results in internuclear gene silencing of inf1. Although silencing is regulated at the transcriptional level, it also affects transcription from other ...
Li, S. +7 more
core +1 more source
The Powdery Mildew Effector CSEP0027 Interacts With Barley Catalase to Regulate Host Immunity
Powdery mildew is one of the most important fungal pathogen diseases. The genome of barley mildew fungus, Blumeria graminis f. sp. hordei (Bgh), encodes a large number of candidate secreted effector proteins (CSEPs). So far, the function and mechanism of
Hongbo Yuan +17 more
doaj +1 more source
RNA interference is ineffective as a routine method for gene silencing in chick embryos as monitored by fgf8 silencing. [PDF]
The in vivo accessibility of the chick embryo makes it a favoured model system for experimental developmental biology. Although the range of available techniques now extends to miss-expression of genes through in ovo electroporation, it remains difficult
Bueno i Torrens, David, 1965- +1 more
core +4 more sources
Influence of retinoblastoma-related gene silencing on the initiation of DNA replication by African cassava mosaic virus Rep in cells of mature leaves in Nicotiana benthamiana plants [PDF]
Background Geminiviruses mainly infect terminally differentiated tissues and cells in plants. They need to reprogramme host cellular machinery for DNA replication.
Yule Liu +14 more
core +1 more source
Viral miRNAs confer survival in host cells by targeting apoptosis related host genes
miRNAs are small non-coding RNAs that regulate gene expression by RNA silencing. Like eukaryotic organisms, some viruses also produce miRNAs. While contribution of host miRNA in preventing viral pathogenesis has been studied, how viral miRNA confers ...
Md Sajedul Islam +1 more
doaj +1 more source
Involvement of cathepsin B in the plant disease resistance hypersensitive response [PDF]
A diverse range of plant proteases are implicated in pathogen perception and in subsequent signalling and execution of disease resistance. We demonstrate, using protease inhibitors and virus-induced gene silencing (VIGS), that the plant papain cysteine ...
Shaw, Jane +49 more
core +1 more source
Suppression of RNA silencing by a plant DNA virus satellite requires a host calmodulin-like protein to repress RDR6 expression. [PDF]
In plants, RNA silencing plays a key role in antiviral defense. To counteract host defense, plant viruses encode viral suppressors of RNA silencing (VSRs) that target different effector molecules in the RNA silencing pathway.
Fangfang Li +3 more
doaj +1 more source
A Method to Produce vsiRNAs in Plants with Cross-Kingdom Gene Silencing Capacity
Plants have evolved defense mechanisms to suppress viral transcription and replication by transcriptional and post-transcriptional gene silencing mediated by virus-derived small interfering RNAs (vsiRNAs).
Hernán de Jesús Villanueva-Alonzo +11 more
doaj +1 more source
The role of TNRC6 proteins in gene silencing [PDF]
Proteins of the GW182 family have recently emerged as key players in miRNAmediated gene silencing. They have been shown to interact with Argonaute proteins, components of the RISC and are assumed to mediate the repression in metazoa. Three paralogues are
Zipprich, Jakob Theophil
core +1 more source

