Results 271 to 280 of about 122,515 (310)

Defining the identity of human adipose-derived mesenchymal stem cells

Biochemistry and Cell Biology, 2015
Adipose-derived mesenchymal stem cells (ADMSCs) are an ideal population for regenerative medical application. Both the isolation procedure and the culturing conditions are crucial steps, since low yield can limit further cell therapies, especially when minimal adipose tissue harvests are available for cell expansion.
Montelatici E., Baluce B., Ragni E., Lavazza C., Parazzi V., Mazzola R., Cantarella G., Brambilla M., Giordano R., Lazzari L.   +9 more
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Epithelial Differentiation of Human Adipose-Derived Stem Cells

2010
The versatile differentiation potential of adipose-derived stem cells (ASC) into cells of mesodermal, entodermal, and ectodermal origin places these cells at the forefront of cell-based therapies and cell transplantation. Epithelial differentiation of ASC may either be initiated by direct cell-cell or cell-matrix contacts, by chemical factors like ...
Patrick C, Baer, Martin, Brzoska, Helmut, Geiger   +2 more
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Human Adipose-Derived Stem Cells with Great Therapeutic Potential

Current Stem Cell Research & Therapy, 2019
: The potential use of stem cell-based therapies for the repair and regeneration of various tissues and organs offers a paradigm shift in regenerative medicine. The use of either embryonic stem cells (ESC) or induced pluripotent stem cells (iPSC) in clinical situations is limited because of regulations and ethical considerations even though these ...
Mohsen, Sheykhhasan, Joanna K L, Wong, Alexander M, Seifalian   +2 more
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The effect of C3 transferase on human adipose-derived stem cells

Human Cell, 2011
Human adipose-derived stem cells (ASCs) are adult pluripotent stem cells, which have the ability to differentiate into fat, cartilage, bone, or nerves that can be applied in tissue engineering. On the other hand, the exoenzyme C3 transferase (C3) is a Rho inhibitor.
Natsuko, Kakudo, Satoshi, Kushida, Kenji, Suzuki, Nagisa, Matsumoto, Kenji, Kusumoto   +4 more
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The Generation and the Manipulation of Human Multipotent Adipose-Derived Stem Cells

2010
In this chapter, we describe a method to isolate and to expand multipotent adipose-derived stem (hMADS) cells from human adipose tissue. We also describe culture conditions to differentiate them into adipocytes at a high rate. This culture system provides a powerful means for studying the first steps of human adipose cell development and a route for ...
Wdziekonski, Brigitte, Mohsen-Kanson, Tala, Villageois, Phi, Dani, Christian   +3 more
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Application of Human Adipose-Derived Stem cells for Bone Regeneration of the Skull in Humans

Journal of Craniofacial Surgery, 2021
Background: Archeological archives report cranioplasty as 1 of the oldest surgical procedures; however, it was not until the last century that true advances have been made. Alternative approaches are necessary to achieve optimal closure of the defect with fewer adverse effects.
Ricardo A, Torres-Guzman, Maria T, Huayllani, Francisco R, Avila, Karla, Maita, Abba C, Zubair, Alfredo, Quinones-Hinojosa, Rachel, Sarabia-Estrada, Antonio J, Forte   +7 more
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Isolation of Human Adipose-Derived Stem Cells from Lipoaspirates

2018
Adipose tissue is as an abundant and accessible source of stem cells with multipotent properties suitable for tissue engineering and regenerative medical applications. Here, we describe methods from our own laboratory and the literature for the isolation and expansion of adipose-derived stem cells (ASCs). We present a large scale procedure suitable for
Jie, Li, J Lowry, Curley, Z Elizabeth, Floyd, Xiying, Wu, Yuan Di C, Halvorsen, Jeffrey M, Gimble   +5 more
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Epigenetic regulation of human adipose-derived stem cells differentiation

Molecular and Cellular Biochemistry, 2015
Adult stem cells have more restricted differentiation potential than embryonic stem cells (ESCs), but upon appropriate stimulation can differentiate into cells of different germ layers. Epigenetic factors, including DNA modifications, take a significant part in regulation of pluripotency and differentiation of ESCs.
Kristina, Daniunaite, Inga, Serenaite, Roberta, Misgirdaite, Juozas, Gordevicius, Ausra, Unguryte, Sandrine, Fleury-Cappellesso, Eiva, Bernotiene, Sonata, Jarmalaite   +7 more
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Isolation of Human Adipose-Derived Stem Cells

Human adipose-derived stromal/stem cells (hASCs) are a promising source of adult stem cells used in numerous applications in regenerative medicine. We present the protocols from our laboratory for isolating and expanding hASCs. The isolation of hASCs involves the enzymatic digestion of adipose tissue and subsequent culturing of the isolated cells.
Elizabeth, Montano, Sara, Al-Ghadban, Bruce A, Bunnell   +2 more
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Methods for the Purification and Characterization of Human Adipose-Derived Stem Cells

2010
Peripheral adipose tissue contains a population of clonogenic precursor cells referred to as adipose-derived stem cells (ASC) that retain the capacity to differentiate into multiple cell types including osteoblasts, adipocytes, chondrocytes, myocytes, and neuronal cells following ex vivo expansion.
Gronthos, S., Zannettino, A.
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