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Isolation of Human Adipose-Derived Stem Cells from Lipoaspirates

2010
Adipose tissue is as an abundant and accessible source of stem cells with multipotent properties suitable for tissue engineering and regenerative medical applications. Here, we describe methods from our own laboratory and the literature for the isolation and expansion of adipose-derived stem cells (ASCs). We present a large-scale procedure suitable for
Gang, Yu   +4 more
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Negative Neuronal Differentiation of Human Adipose-Derived Stem Cell Clones

Regenerative Medicine, 2014
Adipose mesenchymal stem cells are a heterogeneous population. Therefore, the question posed in this study is whether the heterogenic differentiation potential exhibited by the different clones toward mesodermic lineages can be extended to nonmesodermic lineages, such as the neuroectoderm.Different single cell clones of human adipose mesenchymal stem ...
Arribas, María I.   +6 more
openaire   +3 more sources

The Effect of Hyperbaric Oxygen Therapy on Human Adipose-Derived Stem Cells

Plastic & Reconstructive Surgery, 2020
Background: Adipose-derived stem cells are considered as candidate cells for regenerative plastic surgery. Measures to influence cellular properties and thereby direct their regenerative potential remain elusive. Hyperbaric oxygen therapy—the exposure to 100% oxygen at an increased atmospheric pressure—has been propagated as a ...
Yoshinoya, Yuriko   +6 more
openaire   +2 more sources

Adipose-derived Stem cells and BMP2: Part 2. BMP2 may not influence the osteogenic fate of human adipose-derived stem cells

Connective Tissue Research, 2010
Although recent studies have proposed that human adipose-derived stem cells (ASCs), together with BMP2, can heal critical-sized bony defects, a companion study in this issue suggests that ASCs may not respond to BMP2 in vivo. To examine why this may be occurring.
Patricia, Zuk   +4 more
openaire   +2 more sources

An Overview of Neural Differentiation Potential of Human Adipose Derived Stem Cells

Stem Cell Reviews and Reports, 2015
There is wide interest in application of adult stem cells due to easy to obtain with a minimal patient discomfort, capable of producing cell numbers in large quantities and their immunocompatible properties without restriction by ethical concerns. Among these stem cells, multipotent mesenchymal stem cells (MSCs) from human adipose tissue are considered
Hossein, Salehi   +3 more
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In Vitro Expansion of Human Adipose-Derived Stem Cells (ASCs)

Adipose-derived stromal/stem cells (ASCs) have raised a great deal of interest in research and regenerative medicine with potential applications in the treatment of several diseases. ASCs are often required in large numbers for use in research and clinical applications.
Ladislaus Tapiwa, Adam   +2 more
openaire   +2 more sources

Differentiation of Human Adipose-Derived Stem Cells into Cardiomyocytes

2012
The delivery of autologous progenitor cells into injured tissue is emerging as a novel therapeutic option for use in tissue repair and regeneration. Among them, human adipose-derived stem cells (ADSCs) are promising cell source for potential stem cell-based clinical therapies.
Ung Kim, Dong-Gu Shin
openaire   +1 more source

FKBP5 Regulates the Osteogenesis of Human Adipose-derived Mesenchymal Stem Cells

Current Medical Science
Human adipose-derived stem cells (ASCs) have shown considerable potential for tissue regeneration. FK506 binding protein (FKBP) 5 is a cochaperone of several proteins. The purpose of this work was to explore the function of FKBP5 in ASC osteogenesis.Lentivirus infection was used to overexpress or knock down FKBP5 in ASCs.
Xiao-Yu, Tian   +7 more
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[Morphological characteristics of human adipose-derived stem cells].

Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi, 2012
This paper is aimed to isolate and to cultivate human adipose-derived stem cells (hADSCs) from the adipose tissue by a combination of collagenase digestion, adherence to flasks and monoclonal cultural method so as to observe the morphological characteristics of the hADSCs. The immunophenotypes of hADSCs were detected by flow cytometry techniques.
Xu, He   +6 more
openaire   +1 more source

Effect of Culture Conditions on the Multinucleation of Human Adipose-Derived Stem Cells

Journal of Biomaterials and Tissue Engineering, 2015
Adipose-derived stem cells (ASCs) constitute a promising source for cell therapy and tissue engineering approaches as they can be easily obtained in large quantities with minimal patient discomfort. Moreover, ASCs have multilineage differentiation capacity. Among these, differentiation capacity along the myogenic lineage is of particular interest since
openaire   +1 more source

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