Results 11 to 20 of about 165,036 (300)

Chemically defined generation of human cardiomyocytes [PDF]

Nature Methods, 2014
Existing methods for human induced pluripotent stem cell (hiPSC) cardiac differentiation are efficient but require complex, undefined medium constituents that hinder further elucidation of the molecular mechanisms of cardiomyogenesis. Using hiPSCs derived under chemically defined conditions on synthetic matrices, we systematically developed an ...
Paul W Burridge, Elena Matsa, Praveen Shukla, Ziliang C Lin, Jared M Churko, Antje D Ebert, Feng Lan, Sebastian Diecke, Bruno Huber, Nicholas M Mordwinkin, Jordan R Plews, Oscar J Abilez, Bianxiao Cui, Joseph D Gold, Joseph C Wu   +14 more
openaire   +4 more sources

Comparative Effect of MSC Secretome to MSC Co-culture on Cardiomyocyte Gene Expression Under Hypoxic Conditions in vitro

Frontiers in Bioengineering and Biotechnology, 2020
IntroductionDespite major leaps in regenerative medicine, the regeneration of cardiomyocytes after ischemic conditions remains to elucidate. It is crucial to understand hypoxia induced cellular mechanisms to provide advanced treatment options, including ...
Nina Kastner, Julia Mester-Tonczar, Johannes Winkler, Denise Traxler, Andreas Spannbauer, Beate M. Rüger, Georg Goliasch, Noemi Pavo, Mariann Gyöngyösi, Katrin Zlabinger   +9 more
doaj   +1 more source

Empagliflozin and Dapagliflozin Increase Na⁺ and Inward Rectifier K⁺ Current Densities in Human Cardiomyocytes Derived from Induced Pluripotent Stem Cells (hiPSC-CMs)

Cells, 2022
Dapagliflozin (dapa) and empagliflozin (empa) are sodium-glucose cotransporter-2 inhibitors (SGLT2is) that reduce morbidity and mortality in heart failure (HF) patients.
María Dago, Teresa Crespo-García, Anabel Cámara-Checa, Josu Rapún, Marcos Rubio-Alarcón, María Marín, Juan Tamargo, Ricardo Caballero, Eva Delpón   +8 more
doaj   +1 more source

Vacuolated cardiomyocytes in human endomyocardial biopsy specimens [PDF]

Journal of Cardiology Cases, 2020
We encountered an unfamiliar finding during electron microscopic examination of an endomyocardial biopsy obtained from a 55-year-old woman suffering from heart failure due to dilated phase hypertrophic cardiomyopathy. Many cardiomyocytes contained large vacuoles that were mainly empty except for small amounts of amorphous substrate.
Genzou, Takemura, Kenji, Onoue, Takanori, Arimoto, Tetsu, Watanabe, Akiko, Tsujimoto, Chihiro, Takada, Hideshi, Okada, Tomoya, Nakano, Yasuhiro, Sakaguchi, Nagisa, Miyazaki, Takatomo, Watanabe, Hiromitsu, Kanamori, Shinji, Ogura, Yoshihiko, Saito, Takako, Fujiwara, Hisayoshi, Fujiwara, Yasuaki, Hotta   +16 more
openaire   +2 more sources

Single-cell transcriptomic profiling reveals specific maturation signatures in human cardiomyocytes derived from LMNB2-inactivated induced pluripotent stem cells

Frontiers in Cell and Developmental Biology, 2022
Mammalian cardiomyocyte maturation entails phenotypic and functional optimization during the late fetal and postnatal phases of heart development, both processes driven and coordinated by complex gene regulatory networks.
Jie Wang, William Morgan, William Morgan, William Morgan, Ankur Saini, Ankur Saini, Ankur Saini, Tao Liu, John Lough, John Lough, Lu Han, Lu Han, Lu Han   +12 more
doaj   +1 more source

Preconditioning human cardiomyocytes and endothelial cells [PDF]

The Journal of Thoracic and Cardiovascular Surgery, 1998
The effects of simulated "ischemia" and "reperfusion" were evaluated in cell cultures of human ventricular cardiomyocytes and human saphenous vein endothelial cells.Myocyte and endothelial cell cultures were exposed to a low volume (1.5 ml) of either hypoxic (oxygen tension = 16 mm Hg) or anoxic (oxygen tension = 0 mm Hg) phosphate-buffered saline ...
Shirai, Toshizumi, Rao, Vivek, Weisel, Richard D., Ikonomidis, John S., Li, Ren-Ke, Tumiati, Laura C., Merante, Frank, Mickle, Donald A.   +7 more
openaire   +2 more sources

Live-Cell Imaging of the Contractile Velocity and Transient Intracellular Ca²⁺ Fluctuations in Human Stem Cell-Derived Cardiomyocytes

Cells, 2022
Live-cell imaging techniques are essential for acquiring vital physiological and pathophysiological knowledge to understand and treat heart disease. For live-cell imaging of transient alterations of [Ca2+]i in human cardiomyocytes, we engineered human ...
Aviseka Acharya, Harshal Nemade, Krishna Rajendra Prasad, Khadija Khan, Jürgen Hescheler, Nick Blackburn, Ruth Hemmersbach, Symeon Papadopoulos, Agapios Sachinidis   +8 more
doaj   +1 more source

Two dimensional electrophysiological characterization of human pluripotent stem cell-derived cardiomyocyte system. [PDF]

, 2017
Stem cell-derived cardiomyocytes provide a promising tool for human developmental biology, regenerative therapies, disease modeling, and drug discovery.
Gimzewski, James K, Minami, Itsunari, Nakano, Atsushi, Nakano, Haruko, Nakatsuji, Norio, Scharnhorst, Kelsey S, Stieg, Adam Z, Zhu, Huanqi   +7 more
core   +2 more sources

Global DNA methylation and transcriptional analyses of human ESC-derived cardiomyocytes. [PDF]

, 2014
With defined culture protocol, human embryonic stem cells (hESCs) are able to generate cardiomyocytes in vitro, therefore providing a great model for human heart development, and holding great potential for cardiac disease therapies.
Benner, Christopher, Crespo, Isaac, Del Sol, Antonio, Esteban, Concepcion Rodriguez, Gu, Ying, Izpisua Belmonte, Juan Carlos, Li, Mo, Liu, Guang-Hui, Montserrat, Nuria, Plongthongkum, Nongluk, Qu, Jing, Suzuki, Keiichiro, Yang, Jiping, Yi, Fei, Zhang, Kun, Zhang, Weiqi   +15 more
core   +3 more sources

Hypoxia Induces Cardiomyocyte Proliferation in Humans

JACC: Basic to Translational Science, 2020
The mammalian heart has a substantial regenerative capacity in the first few days of life mediated by cardiomyocyte proliferation; however, this capacity is lost shortly after birth ([1][1]). Transition from the embryonic to postnatal circulation results in a rapid and substantial increase in ...
Ahmed, Mahmoud S., Sadek, Hesham A.
openaire   +2 more sources