Results 101 to 110 of about 131,982 (283)

Sediment trap preservative choice impacts particle‐associated microbial community composition

Limnology and Oceanography: Methods, EarlyView.
Abstract Understanding and quantifying the microbial contribution to particulate organic matter (POM) flux and carbon cycling in the ocean relies largely on the collection of sinking POM. A critical aspect of evaluating microbial communities on sediment trap‐collected POM is their preservation until recovery and sample processing.
Nicola L. Paul, Colleen A. Durkin, Sebastian Sudek, Matthieu Bressac, Philip W. Boyd, Eric J. Tappa, Claudia R. Benitez‐Nelson, Alyson E. Santoro   +7 more
wiley   +1 more source

Genomic analyses of multidrug-resistant Salmonella Indiana, Typhimurium, and Enteritidis isolates using MinION and MiSeq sequencing technologies.

PLoS ONE, 2020
We sequenced 25 isolates of phenotypically multidrug-resistant Salmonella Indiana (n = 11), Typhimurium (n = 8), and Enteritidis (n = 6) using both MinION long-read [SQK-LSK109 and flow cell (R9.4.1)] and MiSeq short-read (Nextera XT and MiSeq Reagent ...
Zhao Chen, Dai Kuang, Xuebin Xu, Narjol González-Escalona, David L Erickson, Eric Brown, Jianghong Meng   +6 more
doaj   +1 more source

SARS-CoV-2 Sequencing on Illumina MiSeq Using ARTIC Protocol: Part 2 - Illumina DNA Flex Protocol v1

, 2020
This protocol is an adaption of several circulating protocols on SARS-CoV-2 sequencing using the ARTIC protocol and the Illumina Nexterra DNA Flex library prep kit. Its purpose is to simplify things for the average state public health laboratory, using equipment and expertise they currently posess, most likely from their funded PulseNet activities ...
Joel Sevinsky, Arian Nassiri, Heather Blankenship, Erin Young, Kevin Libuit, Kelly Oakeson, Lauren Turner, StaPH-B Consortium   +7 more
openaire   +1 more source

A case of yogurt central line‐associated bloodstream infection in a child with intestinal failure

Nutrition in Clinical Practice, EarlyView.
Abstract Central line‐associated bloodstream infections (CLABSI) are a significant cause of morbidity in children with intestinal failure (IF). Probiotic therapy is discouraged in patients with IF because of the increased risk of bacteremia with the probiotic organism.
Nasiha Rahim, Emily Keller, Ellen Wagner, Andrea Berkemeyer, Timothy Johnson, Daniel Johnson, Timothy Sentongo   +6 more
wiley   +1 more source

Seasonal variations in planktonic food web structure affect stability by shifting the distribution of energy fluxes

Oikos, EarlyView.
Seasonality in temperate ecosystems shapes species phenology, influencing interactions and food web structure. Variations in species richness and biomass affect trophic interaction strength, a crucial factor for community stability, which can be assessed through energy fluxes – an essential indicator of ecosystem function.
Simon Bazin, Isabelle Domaizon, Cécilia Barouillet, Victor Frossard, Arnaud Sentis   +4 more
wiley   +1 more source

Presumed TP53 mosaicism: variants detected using a NGS hereditary cancer multigene panel [PDF]

, 2019
Aims/Context: NGS multigene panels are routinely used to identify germline pathogenic variants in cancer susceptibility genes. In addition, NGS allows the identification of low-level mosaicism events that may not be detectable by conventional Sanger ...
Gonçalves, João, Rodrigues, Márcia, Rodrigues, Pedro, Silva, Catarina, Theisen, Patrícia, Vieira, Luís   +5 more
core  

An improved and extended dual-index multiplexed 16S rRNA sequencing for the Illumina HiSeq and MiSeq platform

BMC Genomic Data
Background Recent advancements in next-generation sequencing (NGS) technology have ushered in significant improvements in sequencing speed and data throughput, thereby enabling the simultaneous analysis of a greater number of samples within a single ...
A.K. Larin, K.M. Klimina, V.A. Veselovsky, E.I. Olekhnovich, M.D. Morozov, D.I. Boldyreva, R.A. Yunes, A.I. Manolov, D.E. Fedorov, A.V. Pavlenko, Y.S. Galeeva, E.V. Starikova, E.N. Ilina   +12 more
doaj   +1 more source

A reassortant H9N2 influenza virus containing 2009 pandemic H1N1 internal-protein genes acquired enhanced pig-to-pig transmission after serial passages in swine [PDF]

, 2017
Avian H9N2 and 2009 pandemic H1N1 (pH1N1) influenza viruses can infect pigs and humans, raising the concern that H9N2: pH1N1 reassortant viruses could emerge.
Ma, Wenjun, Mancera Gracia, José Carlos, Richt, Juergen A, Saelens, Xavier, Van den Hoecke, Silvie, Van Reeth, Kristien   +5 more
core   +2 more sources

Understanding and mitigating some limitations of Illumina© MiSeq for environmental sequencing of Fungi [PDF]

, 2017
AbstractITS-amplicon metabarcode studies using the illumina MiSeq sequencing platform are the current standard tool for fungal ecology studies. Here we report on some of the particular challenges experienced while creating and using a ribosomal RNA gene (rDNA) amplicon library for an ecological study.
Thomas, Dan, Vandegrift, Roo, Bailes, Graham, Roy, Bitty   +3 more
openaire   +1 more source

Aromatic plants, nest bacterial diversity, and nestling condition in Corsican blue tits

Oikos, EarlyView.
According to the ‘nest protection hypothesis', some passerines incorporate fresh aromatic plants into their nests which reduce pathogens that can negatively affect nestlings. We experimentally evaluated the effect of five aromatic plant species on the nest bacterial microbiota of Corsican blue tits Cyanistes caeruleus.
Hélène Dion‐Phénix, Gabrielle Gingras, Claire Doutrelant, Anne Charmantier, Steven W. Kembel, Denis Réale   +5 more
wiley   +1 more source