Results 81 to 90 of about 133,775 (185)

Comparative analysis of Illumina, PacBio, and nanopore for 16S rRNA gene sequencing of rabbit’s gut microbiota

Frontiers in Microbiomes
This research aims to assess whether sequencing the full length of the 16S rRNA gene using PacBio HiFi sequencing and Oxford Nanopore Technology (ONT) platforms outperform Illumina MiSeq platform in providing detailed specie level insights.
Iliyass Biada, María Antonia Santacreu, Oscar González-Recio, Noelia Ibáñez-Escriche   +3 more
doaj   +1 more source

MaxSSmap: A GPU program for mapping divergent short reads to genomes with the maximum scoring subsequence [PDF]

, 2014
Programs based on hash tables and Burrows-Wheeler are very fast for mapping short reads to genomes but have low accuracy in the presence of mismatches and gaps.
Roshan, Usman, Turki, Turki
core   +2 more sources

illumina MiSeq Dual Index Amplicon Sequencing Sample Preparation v1

, 2019
Preparation of PCR products for amplicon sequencing
openaire   +1 more source

A reassortant H9N2 influenza virus containing 2009 pandemic H1N1 internal-protein genes acquired enhanced pig-to-pig transmission after serial passages in swine [PDF]

, 2017
Avian H9N2 and 2009 pandemic H1N1 (pH1N1) influenza viruses can infect pigs and humans, raising the concern that H9N2: pH1N1 reassortant viruses could emerge.
Ma, Wenjun, Mancera Gracia, José Carlos, Richt, Juergen A, Saelens, Xavier, Van den Hoecke, Silvie, Van Reeth, Kristien   +5 more
core   +2 more sources

SARS-CoV-2 Sequencing on Illumina MiSeq Using ARTIC Protocol: Part 2 - Illumina DNA Flex Protocol v1

, 2020
This protocol is an adaption of several circulating protocols on SARS-CoV-2 sequencing using the ARTIC protocol and the Illumina Nexterra DNA Flex library prep kit. Its purpose is to simplify things for the average state public health laboratory, using equipment and expertise they currently posess, most likely from their funded PulseNet activities ...
Joel Sevinsky, Arian Nassiri, Heather Blankenship, Erin Young, Kevin Libuit, Kelly Oakeson, Lauren Turner, StaPH-B Consortium   +7 more
openaire   +1 more source

Genomic analyses of multidrug-resistant Salmonella Indiana, Typhimurium, and Enteritidis isolates using MinION and MiSeq sequencing technologies.

PLoS ONE, 2020
We sequenced 25 isolates of phenotypically multidrug-resistant Salmonella Indiana (n = 11), Typhimurium (n = 8), and Enteritidis (n = 6) using both MinION long-read [SQK-LSK109 and flow cell (R9.4.1)] and MiSeq short-read (Nextera XT and MiSeq Reagent ...
Zhao Chen, Dai Kuang, Xuebin Xu, Narjol González-Escalona, David L Erickson, Eric Brown, Jianghong Meng   +6 more
doaj   +1 more source

An improved and extended dual-index multiplexed 16S rRNA sequencing for the Illumina HiSeq and MiSeq platform

BMC Genomic Data
Background Recent advancements in next-generation sequencing (NGS) technology have ushered in significant improvements in sequencing speed and data throughput, thereby enabling the simultaneous analysis of a greater number of samples within a single ...
A.K. Larin, K.M. Klimina, V.A. Veselovsky, E.I. Olekhnovich, M.D. Morozov, D.I. Boldyreva, R.A. Yunes, A.I. Manolov, D.E. Fedorov, A.V. Pavlenko, Y.S. Galeeva, E.V. Starikova, E.N. Ilina   +12 more
doaj   +1 more source

Understanding and mitigating some limitations of Illumina© MiSeq for environmental sequencing of Fungi [PDF]

, 2017
AbstractITS-amplicon metabarcode studies using the illumina MiSeq sequencing platform are the current standard tool for fungal ecology studies. Here we report on some of the particular challenges experienced while creating and using a ribosomal RNA gene (rDNA) amplicon library for an ecological study.
Thomas, Dan, Vandegrift, Roo, Bailes, Graham, Roy, Bitty   +3 more
openaire   +1 more source

Illumina MiSeq sequencing investigation of Chanhua (Cordyceps cicadae Shing) fungal community structures in different regions

Journal of Traditional Chinese Medical Sciences, 2018
Objective: This study was carried out to elucidate the fungal diversity of Chanhua (Cordyceps cicadae Shing), which is an entomogenous fungal parasite that grows on wingless cicada nymphs.
Tengfei Liu, Hongmei Zhang, Xiaosa Shi, Weiwei Ma, Yancong Zhang, Ziyao Liu, Ying Huang, Xinyuan Shi   +7 more
doaj   +1 more source

Analysis of hypervariable DNA sequences by NGS technologies: QuasiFlow [PDF]

, 2016
The development of Next Generation Sequencing (NGS) technologies has allowed deep characterization of highly variable sequences such as viral or mitochondrial genomes.
Claros-Diaz, Manuel Gonzalo, Díaz Martínez, Luis, Grande-Perez, Ana, Seoane Zonjic, Pedro, Viguera-Minguez, Enrique   +4 more
core