The location of "8"-shaped hatching influences inner cell mass formation in mouse blastocysts. [PDF]
The hatching of a blastocyst where the blastocyst portions on the inside and the outside of the zona pellucida feature a figure-of-eight shape is termed "8"-shaped hatching; this type of hatching has been reported to affect the proper presentation of the
Yohei Onodera +11 more
doaj +2 more sources
The trophectoderm acts as a niche for the inner cell mass through C/EBPα-regulated IL-6 signaling. [PDF]
Gene regulation; Somatic cell reprogramming; TrophectodermRegulación de genes; Reprogramación de células somáticas; TrofoectodermoRegulació de gens; Reprogramació de cèl·lules somàtiques; TrofectodermaIL-6 has been shown to be required for somatic cell ...
Plana-Carmona M +15 more
europepmc +2 more sources
A Haspin promoter element induces tissue-specific methylation of a transcription region and the regulation of gene expression in mouse ova [PDF]
HASPIN is a nuclear serine-threonine kinase originally identified in the mouse testis. Its 193 bp DNA promoter element(hereafter, 193PE) regulates bidirectional, synchronous gene expression in the germ cells of male mice.
Hiromitsu Tanaka, Keizo Tokuhiro
doaj +1 more source
BackgroundRecent studies have demonstrated that both blastocoel fluid (BF) and spent cell culture media (SCM) have potential as materials for non-invasive or less-invasive pre-implantation genetic analysis.
Wenhao Shi +8 more
doaj +1 more source
Mosaicism in the inner cell mass of human blastocysts [PDF]
Although mosaicism was shown to be a normal feature in cleaving embryos, its consequences for the late preimplantation stages are unknown. We performed blastocyst immunosurgery, followed by fluorescent in-situ hybridization (FISH), to determine the number of cells and degree of mosaicism in the inner cell mass (ICM) of human blastocysts.
S, Evsikov, Y, Verlinsky
openaire +2 more sources
This study was designed to the optimize vitrification and in-straw warming protocol of in vitro-produced bovine embryos by comparing two different equilibration periods, short equilibrium (SE: 3 min) and long equilibrium (LE: 12 min).
Iris Martínez-Rodero +10 more
doaj +1 more source
Cell fate clusters in ICM organoids arise from cell fate heredity and division: a modelling approach
During the mammalian preimplantation phase, cells undergo two subsequent cell fate decisions. During the first decision, the trophectoderm and the inner cell mass are formed.
Tim Liebisch +5 more
doaj +1 more source
Trophectoderm regeneration to support full-term development in the inner cell mass isolated from bovine blastocyst. [PDF]
Kohri N +9 more
europepmc +2 more sources
Replacement of inner cell mass in mouse
The intraor inter-strain reconstituted blastocysts were produced by replacing the inner cell mass of Kunming mouse blastocysts with that of Kunming or C57BL/6 mouse strain blastocysts. A total of 192 intra-strain reconstituted blastocysts were transferred into 17 pseudo-pregnant Kunming mice, and 2 reconstituted embryos were developed into term; while ...
Chunming Bi +4 more
openaire +2 more sources
A single-cell level comparison of human inner ear organoids with the human cochlea and vestibular organs [PDF]
Inner ear disorders are among the most common congenital abnormalities; however, current tissue culture models lack the cell type diversity to study these disorders and normal otic development.
Carl Nist-Lund +29 more
core +1 more source

