Results 1 to 10 of about 163,730 (255)

Electrically synchronizing and modulating the dynamics of ERK activation to regulate cell fate

iScience, 2021
Summary: Intracellular signaling dynamics play fundamental roles in cell biology. Precise modulation of the amplitude, duration, and frequency of signaling activation will be a powerful approach to investigate molecular mechanisms as well as to engineer ...
Liang Guo, Kan Zhu, Michael Pargett, Adam Contreras, Patrick Tsai, Quan Qing, Wolfgang Losert, John Albeck, Min Zhao   +8 more
doaj   +3 more sources

A fatal affair: Circulating tumor cell relationships that shape metastasis

iScience, 2021
Summary: Circulating tumor cells are metastatic precursors in several cancer types. Their biology and clinical utility are subject to numerous investigations, yet one aspect that is often neglected is their entanglement with the tumor microenvironment ...
Lauren L. Ozimski, David Gremmelspacher, Nicola Aceto   +2 more
doaj   +3 more sources

Integrative analysis of single-cell RNA-seq and ATAC-seq reveals heterogeneity of induced pluripotent stem cell-derived hepatic organoids

iScience, 2023
Summary: To gain deeper insights into transcriptomes and epigenomes of organoids, liver organoids from two states (expandable and more differentiated) were subjected to single-cell RNA-seq (scRNA-seq) and single-cell ATAC-seq (scATAC-seq) analyses ...
Jong-Hwan Kim, Seon Ju Mun, Jeong-Hwan Kim, Myung Jin Son, Seon-Young Kim   +4 more
doaj   +3 more sources

Functional Heterogeneity of Mouse Prostate Stromal Cells Revealed by Single-Cell RNA-Seq

iScience, 2019
Summary: We perform a single-cell RNA sequencing analysis to investigate the phenotypic and functional heterogeneity of the adult mouse prostate stromal cells.
Oh-Joon Kwon, Yiqun Zhang, Yumei Li, Xing Wei, Li Zhang, Rui Chen, Chad J. Creighton, Li Xin   +7 more
doaj   +3 more sources

Decoding functional and developmental trajectories of tissue-resident uterine dendritic cells through integrative omics

iScience
Summary: Uterine dendritic cells (uDCs) are critical for endometrial function, yet their origin and functional roles during the pre- and post-implantation periods in the human endometrium remain largely unknown.
Aditi Singh, Jing Yang, Nicholas K. Adzibolosu, Songchen Cai, Elana Kauf, Lingtao Yang, Qiyuan Li, Hanjie Li, Alexandra Werner, Siddharth Parthasarathy, Jiahui Ding, Jared Fortier, Marta Rodriguez-Garcia, Lianghui Diao, Gil Mor   +14 more
doaj   +3 more sources

Sequence-directed concentration of G protein-coupled receptors in COPII vesicles

iScience, 2023
Summary: G protein-coupled receptors (GPCRs) constitute the largest superfamily of plasma membrane signaling proteins. However, virtually nothing is known about their recruitment to COPII vesicles for forward delivery after synthesis in the endoplasmic ...
Xin Xu, Nevin A. Lambert, Guangyu Wu
doaj   +1 more source

Contribution of septins to human platelet structure and function

iScience, 2022
Summary: Although septins have been well-studied in nucleated cells, their role in anucleate blood platelets remains obscure. Here, we elucidate the contribution of septins to human platelet structure and functionality. We show that Septin-2 and Septin-9
Oleg V. Kim, Rustem I. Litvinov, Elmira R. Mordakhanova, Erfei Bi, Olga Vagin, John W. Weisel   +5 more
doaj   +1 more source

Glycosylation network mapping and site-specific glycan maturation in vivo

iScience, 2022
Summary: Glycoprotein processing along a complex highly compartmentalized pathway is a hallmark of eukaryotic cells. We followed the kinetics of intracellular, site-specific glycan processing of a model protein with five distinct N-glycosylation sites ...
Marie-Estelle Losfeld, Ernesto Scibona, Chia-wei Lin, Markus Aebi   +3 more
doaj   +1 more source

Integration and Iteration: Using Advanced, High-Content Imaging and Single-Cell Gene Expression Analysis to Uncover Unique Aspects of Follicular Lymphoma Biology

Blood, 2020
BACKGROUND: Follicular lymphoma (FL) is an indolent malignancy of germinal center B-cell origin. FL patients experience remarkable heterogeneity in their disease trajectory, with many patients slowly progressing over several years, and a subset of patients experiencing an aggressive clinical course.
Andrea J. Radtke, Arthur L. Shaffer, Stefania Pittaluga, Alexander Bagaev, Nishant Thakur, Theresa Davies-Hill, Olga Plotnikova, Ilia Galkin, Ekaterina Postovalova, Viktor Svekolkin, Sergei Isaev, Yaroslav Lozinsky, Mark Meerson, Arina Varlamova, Pavel Ovcharov, Margarita Polyakova, Krystle Nomie, Ekaterina Nuzhdina, Nikita Kotlov, Maria Tsiper, Felix Frenkel, Ravshan Attaulakhanov, Nathan H Fowler, Ziv Yaniv, Bradley C Lowekamp, Michael Kelly, Ezzat Dadkhah, Da Wei Huang, Jagan R. Muppidi, Elaine S. Jaffe, Wyndham H. Wilson, Louis M. Staudt, Ronald N. Germain, Mark Roschewski   +33 more
openaire   +1 more source

Population dynamics of EMT elucidates the timing and distribution of phenotypic intra-tumoral heterogeneity

iScience, 2023
Summary: The Epithelial-to-Mesenchymal Transition (EMT) is a hallmark of cancer metastasis and morbidity. EMT is a non-binary process, and cells can be stably arrested en route to EMT in an intermediate hybrid state associated with enhanced tumor ...
Annice Najafi, Mohit K. Jolly, Jason T. George   +2 more
doaj   +1 more source