Results 21 to 30 of about 165 (150)

Single-cell transcriptome atlas of adult male and female human hookworm Ancylostoma ceylanicum

iScience
Summary: Hookworms infect over 500 million people globally and are a major neglected tropical disease. Their high prevalence and reinfection rates, combined with emerging drug resistance, highlight the need for new therapeutic or vaccine targets.
Suman Bharti, Bruce A. Rosa, John Martin, Makedonka Mitreva   +3 more
doaj   +1 more source

Integrative modeling of the cell

Acta Biochimica et Biophysica Sinica, 2022
A whole-cell model represents certain aspects of the cell structure and/or function. Due to the high complexity of the cell, an integrative modeling approach is often taken to utilize all available information including experimental data, prior knowledge
Zhong Xianni, Zhao Jihui, Sun Liping
doaj   +1 more source

An unexpected alternative viologen electron mediator site in tungsten‐containing formate dehydrogenase

FEBS Letters, EarlyView.
An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley   +1 more source

A cell atlas of multiple liver organoids and the fetal liver based on scRNA-seq

iScience
Summary: Understanding the cellular characteristics and regulatory networks of cells during the growth of human liver organoids is crucial for comprehending liver development and function.
Qinfeng Ma, Xu Zhang, Jianbo Pan
doaj   +1 more source

Cell geometry and membrane protein crowding constrain Escherichia coli growth rate, overflow metabolism, respiration, and maintenance energy

FEBS Letters, EarlyView.
The physical dimensions and shape of bacterial cells define the surface area available to acquire nutrients and the volume available for synthesizing proteins and DNA. Here, we use computational systems biology to decode the importance of cell geometry as a major determinant of prokaryotic phenotype, including growth rate and metabolic efficiency. This
Ross P. Carlson, Tomáš Gedeon, Mauricio Garcia Benitez, Campbell Putnam, William R. Harcombe, Radhakrishnan Mahadevan, Ashley E. Beck   +6 more
wiley   +1 more source

A transfer learning framework to elucidate the clinical relevance of altered proximal tubule cell states in kidney disease

iScience
Summary: The application of single-cell technologies in clinical nephrology remains elusive. We generated an atlas of transcriptionally defined cell types and cell states of human kidney disease by integrating single-cell signatures reported in the ...
David Legouis, Anna Rinaldi, Daniele Malpetti, Gregoire Arnoux, Thomas Verissimo, Anna Faivre, Francesca Mangili, Andrea Rinaldi, Lorenzo Ruinelli, Jerome Pugin, Solange Moll, Luca Clivio, Marco Bolis, Sophie de Seigneux, Laura Azzimonti, Pietro E. Cippà   +15 more
doaj   +1 more source

Microbiome−host proteostasis crosstalk—An emerging perspective on mechanisms and interventions toward healthy longevity

FEBS Letters, EarlyView.
Proteostasis and the gut microbiota play a key role in shaping host physiology. Microbiota‐derived metabolites, vitamins, and RNA modulate host proteostasis. Findings from model systems, including C. elegans, indicate microbes can either stabilize or disrupt host proteostasis.
Abhishek Anil Dubey, Maria Ermolaeva
wiley   +1 more source

Dual Feedforward Loops Modulate Type I Interferon Responses and Induce Selective Gene Expression during TLR4 Activation

iScience, 2020
Summary: Although the dynamic features of type I coherent feedforward loop (C1-FFL) has been well studied, how C1-FFL shapes cell-to-cell heterogeneity remains unclear.
Jie Zhou, Tingzhe Sun, Shouheng Jin, Zhiyong Guo, Jun Cui   +4 more
doaj   +1 more source

Integrative analysis of single-cell RNA-seq and ATAC-seq reveals heterogeneity of induced pluripotent stem cell-derived hepatic organoids

iScience, 2023
Summary: To gain deeper insights into transcriptomes and epigenomes of organoids, liver organoids from two states (expandable and more differentiated) were subjected to single-cell RNA-seq (scRNA-seq) and single-cell ATAC-seq (scATAC-seq) analyses ...
Jong-Hwan Kim, Seon Ju Mun, Jeong-Hwan Kim, Myung Jin Son, Seon-Young Kim   +4 more
doaj   +1 more source

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

FEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa, Shinya Sugimoto, Toshiro Sato   +2 more
wiley   +1 more source