Results 51 to 60 of about 165 (150)

Liquid biopsy‐based diagnostic evaluation of hypermethylated CpG sites for ovarian cancer diagnosis

Molecular Oncology, EarlyView.
This schematic outlines the workflow from biomarker identification to duplex MethyLight assay validation for epithelial ovarian cancer diagnosis using cfDNA‐based liquid biopsy. Initial screening of hypermethylated CpG candidates (cg02957270, cg10061138 cg00480298, COL2A1) was performed in tissue using ARMS‐PCR, COBRA, qPCR and image analysis. Selected
Deepa Bisht, Sameer Gupta, Amrita Chaurasia, Manisha Sachan   +3 more
wiley   +1 more source

Genome Architecture Mediates Transcriptional Control of Human Myogenic Reprogramming

iScience, 2018
Summary: Genome architecture has emerged as a critical element of transcriptional regulation, although its role in the control of cell identity is not well understood.
Sijia Liu, Haiming Chen, Scott Ronquist, Laura Seaman, Nicholas Ceglia, Walter Meixner, Pin-Yu Chen, Gerald Higgins, Pierre Baldi, Steve Smale, Alfred Hero, Lindsey A. Muir, Indika Rajapakse   +12 more
doaj   +1 more source

Astrocyte heterogeneity in brain metastases

Molecular Oncology, EarlyView.
Astrocytes emerge as pivotal regulators of metastatic colonization, survival, immune remodeling, and therapy response associated with an increasing heterogeneity that requires spatially and longitudinally resolved approaches to uncover regulatory programs and guide context‐specific therapies.
Carolina Hernández‐Oliver, Neibla Priego, Manuel Valiente   +2 more
wiley   +1 more source

Epigenetic control of myogenic identity of human muscle stem cells in Duchenne muscular dystrophy

iScience
Summary: In Duchenne muscular dystrophy (DMD), muscle stem cells’ (MuSCs) regenerative capacities are overwhelmed leading to fibrosis. Whether MuSCs have intrinsic defects or are disrupted by their environment is unclear.
Jimmy Massenet, Michèle Weiss-Gayet, Hina Bandukwala, Wilhelm Bouchereau, Stéphanie Gobert, Mélanie Magnan, Arnaud Hubas, Patrick Nusbaum, Isabelle Desguerre, Cyril Gitiaux, F. Jeffrey Dilworth, Bénédicte Chazaud   +11 more
doaj   +1 more source

From tumor‐centric to ecosystem‐based hypotheses in brain tumor research and care

Molecular Oncology, EarlyView.
Primary brain tumors, whether in adults or children, present a major challenge because of their dramatic prognosis and the ongoing lack of efficient therapeutic approaches. In recent years, a shift has occurred from tumor‐centric concepts to a more holistic view of these tumors as dynamic ecosystems.
Julie Gavard, Flavie Bénard, Eva Billat, Margaux Le Guyon, Victoria Maltret, Laura Merlet, Chloé Astrid Walter, Tony Avril, Eric Chevet   +8 more
wiley   +1 more source

The transcriptome of early compensatory kidney growth reveals cell and time specific responses

iScience
Summary: Following kidney removal, the remaining kidney enlarges and increases its function. The mechanism and signals driving this compensatory kidney hypertrophy and the enlargement of its constituent kidney cells remains elusive.
Darling M. Rojas-Canales, Soon Wei Wong, Elise J. Tucker, Anthony O. Fedele, Kym McNicholas, Anne-Sophie Mehdorn, Jonathan M. Gleadle   +6 more
doaj   +1 more source

Single‐cell DNA methylation profiling: Technologies, computation, and applications in precision oncology

Molecular Oncology, EarlyView.
Single‐cell DNA methylation (scDNAme) profiling maps epimutational clonal evolution, revealing mechanisms of malignancy and therapeutic resistance across diverse cancer types. By providing a high‐resolution landscape of intratumoral heterogeneity, these technologies empower precise patient stratification, guide the development of enhanced ...
Ik Soo Kim
wiley   +1 more source

3D imaging and single-cell analysis reveal cellular heterogeneity of lymphatic valve endothelial cell types

iScience
Summary: Lymphatic vasculature has specialized vessels to support unidirectional interstitial fluid transport and immune surveillance. Although the lymphatic system is a critical actor in skin homeostasis, the spatial organization of the lymphatic ...
Emmanuelle Marchaud, Renaud Morin, Jason S. Iacovoni, Tangra Draia-Nicolau, Aurélie Gomes, Marine Norlund, Pascale Bernes-Lasserre, Jean-Michel Lagarde, Anne-Catherine Prats, Barbara Garmy-Susini, Anne Bouloumie, Anaïs Briot, Florence Tatin   +12 more
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

scRNA-seq reveals the diversity of the developing cardiac cell lineage and molecular players in heart rhythm regulation

iScience
Summary: We utilized scRNA-seq to delineate the diversity of cell types in the zebrafish heart. Transcriptome profiling of over 50,000 cells at 48 and 72 hpf defined at least 18 discrete cell lineages of the developing heart.
Karim Abu Nahia, Agata Sulej, Maciej Migdał, Natalia Ochocka, Richard Ho, Bożena Kamińska, Marcin Zagorski, Cecilia Lanny Winata   +7 more
doaj   +1 more source