Results 71 to 80 of about 41,506 (219)

A population model reveals a surprising role of stochastic cell division in epigenetic memory systems

iScience
Summary: Epigenetic memory systems store transient environmental signals in bacteria in specific DNA methylation patterns. Synthetic zinc finger protein (ZnF4) binds to the DNA in a methylation-dependent manner and represses the expression of the DNA ...
Viviane Klingel, Dimitri Graf, Sara Weirich, Albert Jeltsch, Nicole E. Radde   +4 more
doaj   +1 more source

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Single-cell transcriptomic reveals the peritoneal microenvironmental change in long-term peritoneal dialysis patients with ultrafiltration failure

iScience
Summary: The microenvironmental changes in peritoneal dialysis effluent (PDE) after long-term vintage (LV) of PD in patients with ultrafiltration failure (LV_UF) are unclear.
Xiangwen Diao, Cuixia Zhan, Hongjian Ye, Haishan Wu, Chunyan Yi, Jianxiong Lin, Haiping Mao, Wei Chen, Xiao Yang   +8 more
doaj   +1 more source

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

Transcriptomic signatures of progressive and regressive liver fibrosis and portal hypertension

iScience
Summary: Persistent liver injury triggers a fibrogenic program that causes pathologic remodeling of the hepatic microenvironment (i.e., liver fibrosis) and portal hypertension.
Oleksandr Petrenko, Philipp Königshofer, Ksenia Brusilovskaya, Benedikt S. Hofer, Katharina Bareiner, Benedikt Simbrunner, Frank Jühling, Thomas F. Baumert, Joachim Lupberger, Michael Trauner, Stefan G. Kauschke, Larissa Pfisterer, Eric Simon, André F. Rendeiro, Laura P.M.H. de Rooij, Philipp Schwabl, Thomas Reiberger   +16 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

From patient advocacy to patient‐driven research: Building active partnerships beginning at the bench to reach the bedside

FEBS Open Bio, EarlyView.
Research is strongest when conducted alongside patients, not just about them. Patient research organizations help integrate patient perspectives into research priorities, study design, and scientific meetings, leading to meaningful patient outcomes and development of relevant therapies.
Jenica H. Kakadia, Rachel Heilmann, Maximiliano Barrientos, Laura MacNeill, Ingrid Vallee, Sarah K. Schultz, Patrick O'Donoghue, Angelica A. Moresco, Victoria M. Siu, Ilka U. Heinemann   +9 more
wiley   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source

Mutant NPM1 in Acute Myeloid Leukemia Initiation and Maintenance

Aging and Cancer, EarlyView.
NPM1 mutations drive acute myeloid leukemia by acting as neomorphic transcriptional regulators that cooperate with Menin–MLL and XPO1 to sustain HOX/MEIS1 expression and block differentiation. Targeting these mutant‐specific transcriptional dependencies provides a rational therapeutic strategy for NPM1‐mutated AML.
Yanan Jiang, Zhong Fan, Runmeng Liu, Xiaotian Zhang   +3 more
wiley   +1 more source

MFAP5+ synovial fibroblasts drive LOX upregulation to promote osteoarthritis progression

iScience
Summary: Synovial fibroblasts (SFs), the predominant synovial cell population, drive osteoarthritis (OA) pathogenesis. Understanding SF reprogramming in OA progression may reveal potential targets.
Linzhu Wang, Guozhao Wei, Fenghua Ma, Xingyu Li, Huizhen Zhang, Houliang Pan, Zikang Meng, Sen Yang, Ruonan Yang, Juan Dong, Xue Xiong, Zhaoyuan Xu, Boyang Zhang, Hongyi Cheng, Baiyan Li, Lei Yang, Xiaoqiang E, Xuelian Li   +17 more
doaj   +1 more source