Results 71 to 80 of about 5,935 (298)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Simulation-Based Learning in Training Contexts [PDF]

Educaţia 21
This article examines how professionals preparing to become trainers for Școala de Valori/The School of Values association engage in simulation-based learning.
Silvia Bogdan, Natalia-Maria Durușa
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Assessing interactional competence in a superdiverse setting

, 2014
This paper reports from an on-going study on interaction in paired speaking tests.The main data consists of video recorded speaking tests in the final national test in Swedish for adult immigrants (B1/B1+ on the CEFR-scale). The participants have varying
Rydell, Maria,
core   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Interactional Competence or Communicative Competence: Overview of Related Theories

Bulletin of Science and Practice, 2021
The article analyses the relationship of communicative and interactional competences by providing chronological evolution of the terms and discussing contrasting and similar approaches the language scholars presented earlier. Besides, relevant examples for issues of testing and assessing these two competences are illustrated to show the gaps between ...
openaire   +1 more source

Why human connection is the true metric of research success

FEBS Open Bio, EarlyView.
Human‐centred mentorship can be shaped by mentor attributes, actions, intrinsic drive and career ambition. Drawing on reflections across Singapore and France, as well as workshop insights from FEBS‐IUBMB ENABLE 2024, this article shows that human‐centred mentorship creates the conditions for sustainable growth, well‐being and retention in research ...
Timothy Lin Yun Tan, Mattias Wei Ren Kon, Xavier Coumoul, Fun Man Fung   +3 more
wiley   +1 more source

YIPFα1A expression is regulated by multilayered molecular mechanisms

FEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji, Yurika Nakanishi, Nobuhiro Nakamura   +2 more
wiley   +1 more source

What authentic data tell us about pupils’ interactional competence: A video-based interdisciplinary study

Ricerche di Pedagogia e Didattica
This article introduces the Special Issue presenting the first findings of the Italian project ChICaS (Children's Interactional Competence at School: Conversational Social Norms, Forms of Participation, and Language Structures), a PRIN (Progetto di ...
Piera Margutti, Elisa De Roberto, Rosa Pugliese, Elisa Rossi, Daniela Veronesi   +4 more
doaj   +1 more source

LEARNING TO SAY GRACE

Social Interaction, 2018
Based on a detailed analysis of four naturally occurring dinner-table conversations video-recorded over a period of three weeks, this study uses longitudinal Conversation Analysis to track an outsider's growing involvement in the family ritual of praying
Tim Greer
doaj   +1 more source