Virulence-associated genes, resistance genes and adhesion and probiotic activity tested by a new screening method [PDF]
We established an automated screening method to characterize adhesion of Escherichia coli to intestinal porcine epithelial cells (IPEC-J2) and their probiotic activity against infection by enteropathogenic E. coli (EPEC). 104 intestinal E.
Frömmel, Ulrike +13 more
core +1 more source
Effects of Different Methionine Sources on Methionine Metabolism in the IPEC-J2 Cells [PDF]
As one of the essential amino acids, methionine (Met) plays an important role in biological events such as methylation and antioxidant properties besides its function in protein synthesis. Different Met sources have been used in animal production, but their effects on Met metabolic pathways are not well understood. In the present study, we investigated
Fangrui Zuo +4 more
openaire +2 more sources
A Medium-Throughput System for In Vitro Oxidative Stress Assessment in IPEC-J2 Cells [PDF]
The feed industry continuously seeks new molecules with antioxidant capacity since oxidative stress plays a key role in intestinal health. To improve screening of new antioxidants, this study aims to set up an assay to assess oxidative stress in the porcine small intestinal epithelial cell line IPEC-J2 using plate-reader-based analysis of fluorescence.
Miriam Ayuso +2 more
openaire +3 more sources
Deoxynivalenol (DON) is universally detected trichothecene in most cereal commodities, which is considered as a major hazardous material for human and animal health.
Cong Zhang +9 more
doaj +1 more source
Gentamicin sulphate permeation through porcine intestinal epithelial cell monolayer [PDF]
Gentamicin is an aminoglycoside antibiotic widely used in combination with dimethyl sulphoxide (DMSO) in topical drug formulations. It is not known, however, whether DMSO can enhance the permeation of gentamicin through biological membranes, leading to ...
Al-Amoud A. I. +27 more
core +1 more source
Deoxynivalenol Induces Inflammation in IPEC-J2 Cells by Activating P38 Mapk And Erk1/2 [PDF]
Fusarium-derived mycotoxin deoxynivalenol (DON) usually induces diarrhea, vomiting and gastrointestinal inflammation. We studied the cytotoxic effect of DON on porcine small intestinal epithelium using the intestinal porcine epithelial cell line IPEC-J2.
Hua Zhang +4 more
openaire +3 more sources
Background Enterotoxigenic Escherichia coli (ETEC) K88 commonly colonize in the small intestine and keep releasing enterotoxins to impair the intestinal barrier function and trigger inflammatory reaction. Although Lactobacillus salivarius (L. salivarius)
Jiayun Qiao +3 more
doaj +1 more source
Stress induced Salmonella Typhimurium recrudescence in pigs coincides with cortisol induced increased intracellular proliferation in macrophages [PDF]
Salmonella Typhimurium infections in pigs often result in the development of carriers that intermittently excrete Salmonella in very low numbers. During periods of stress, for example transport to the slaughterhouse, recrudescence of Salmonella may occur,
Elin Verbrugghe +9 more
core +2 more sources
Air–liquid interface enhances oxidative phosphorylation in intestinal epithelial cell line IPEC-J2 [PDF]
AbstractThe intestinal porcine epithelial cell line IPEC-J2, cultured under the air–liquid interface (ALI) conditions, develops remarkable morphological characteristics close to intestinal epithelial cells in vivo. Improved oxygen availability has been hypothesised to be the leading cause of this morphological differentiation.
Sonja Klasvogt +9 more
openaire +3 more sources
Transcriptome analysis reveals modulation of the STAT family in PEDV-infected IPEC-J2 cells [PDF]
Abstract Background Porcine epidemic diarrhea virus (PEDV) is a causative agent of serious viral enteric disease in suckling pigs. Such diseases cause considerable economic losses in the global swine industry. Enhancing our knowledge of PEDV-induced transcriptomic responses in host cells is imperative to understanding ...
Zhengzheng Hu +6 more
openaire +3 more sources

