Altered Cytokine Expression and Barrier Properties after In Vitro Infection of Porcine Epithelial Cells with Enterotoxigenic Escherichia coli and Probiotic Enterococcus faecium [PDF]
, 2017 The aim of the present study was to elucidate the effects of the probiotic feed additive Enterococcus faecium NCIMB 10415 (E. faecium) on porcine jejunal epithelial cells (IPEC-J2) during an in vitro challenge with enterotoxigenic Escherichia coli (ETEC).
Aschenbach, Jörg R. +5 more
core +3 more sources
Food Frontiers, Volume 7, Issue 3, May 2026.ABSTRACT Magnolol is a natural bioactive phenolic compound with potent antioxidant properties and plays an important role in modulating intestinal healthy. However, the underlying mechanisms of magnolol in alleviating intestinal oxidative stress damage and improving the intestinal barrier integrity remain unknown. Thus, this study aimed to evaluate the
Mengyao Fang +9 more
wiley +1 more source
Doxycycline Induces Mitophagy and Suppresses Production of Interferon-β in IPEC-J2 Cells [PDF]
Frontiers in Cellular and Infection Microbiology, 2017 Previous reports have demonstrated that the second-generation tetracycline derivative doxycycline (DOX) interrupts mitochondrial proteostasis and physiology, inhibits proliferation of many cell types, and induces apoptosis. However, the effects of DOX, which is widely used in porcine husbandry by feed, on the porcine intestinal epithelium are unclear ...
Xing, Yang +4 more
openaire +2 more sources
4-Phenylbutyric acid accelerates rehabilitation of barrier function in IPEC-J2 cell monolayer model
Animal Nutrition, 2021 As the first line of defence against pathogens and endotoxins crossing the intestine-blood barrier, the intestinal epithelial barrier plays a determinant role in pigs' health and growth. 4-Phenylbutyric acid (4-PBA), an aromatic fatty acid, was reported to benefit homeostasis of endoplasmic reticulum and protein synthesis.
Qian Jiang +8 more
openaire +3 more sources
Dihydromyricetin Regulates PANoptosis to Protect Salmonella Typhimurium‐Infected Weaned Piglets
Food Frontiers, Volume 7, Issue 2, March 2026.After DHM pretreatment, piglets were subjected to Salmonella infection. Serum and ileocecal samples from the piglets were collected for TUNEL assay, Western blotting analysis, and metabolomics analysis. The results collectively indicated that DHM could protect piglets from Salmonella infection.
Tingting Xiao +6 more
wiley +1 more source
Rapid production of a chimeric antibody-antigen fusion protein based on 2A-peptide cleavage and green fluorescent protein expression in CHO cells [PDF]
, 2019 To enable large-scale antibody production, the creation of a stable, high producer cell line is essential. This process often takes longer than 6 months using standard limited dilution techniques and is very labor intensive.
Cox, Eric +2 more
core +2 more sources
Effects of 1,2-Dimethylhydrazine on Barrier Properties of Rat Large Intestine and IPEC-J2 Cells [PDF]
, 2021 Colon cancer is accompanied by a decrease of epithelial barrier properties, which are determined by tight junction (TJ) proteins between adjacent epithelial cells.
Amasheh, Salah +3 more
core +1 more source
An Engineered Solidified Peptide Hemocyte Sponge as Nanomotor Storage to Combat Bacterial Colitis
Advanced Science, Volume 13, Issue 8, 9 February 2026.Peptides immobilized via nickel coordination can stabilize the framework structure. Following coating with red blood cell membranes, these constructs are capable of continuously sequestering endotoxins and mitigating colonic pathogenic damage by alleviating bacterial‐induced ferroptosis.
Yuxin Fang +8 more
wiley +1 more source
Inhibition of Salmonella Binding to Porcine Intestinal Cells by a Wood-Derived Prebiotic
Microorganisms, 2020 Numerous Salmonella enterica serovars can cause disease and contamination of animal-produced foods. Oligosaccharide-rich products capable of blocking pathogen adherence to intestinal mucosa are attractive alternatives to antibiotics as these have ...
Aleksandar Božić +10 more
doaj +1 more source
Salmonella Typhimurium exploits inflammation to its own advantage in piglets [PDF]
, 2015 Salmonella Typhimurium (S. Typhimurium) is responsible for foodborne zoonotic infections that, in humans, induce self-limiting gastroenteritis. The aim of this study was to evaluate whether the wild-type strain S.
Barbara eChirullo +14 more
core +3 more sources