Results 41 to 50 of about 3,763 (189)
Quantitative Nucleocytoplasmic Transport Assays in Cellular Models of Neurodegeneration
Bio-Protocol, 2020 Nucleocytoplasmic transport deficits are suggested to play a role in neurodegenerative disorders, including amyotrophic lateral sclerosis (ALS). Given the importance and complexity of this process, understanding when these aberrations occur and which ...
Joni Vanneste +4 more
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iScience, 2023 Summary: Karyopherins mediate the movement between the nucleus and cytoplasm of specific proteins in diverse cellular processes. Through a loss-of-function approach, we here examine the role of Karyopherin Subunit Alpha 2 (Kpna2) in spermatogenesis ...
Paula Navarrete-López +6 more
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Cloning and characterization of human karyopherin β3 [PDF]
Proceedings of the National Academy of Sciences, 1997 Nuclear import of classical nuclear localization sequence-bearing proteins is mediated by karyopherin α/β1 heterodimers. A second nuclear import pathway, mediated by karyopherin β2 (transportin), recently was described for mRNA-binding proteins. Here we report the cloning and characterization of human karyopherin β3, which may be involved in a third ...
N R, Yaseen, G, Blobel
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Modulation of Histone Deposition by the Karyopherin Kap114 [PDF]
Molecular and Cellular Biology, 2005 The nuclear import of histones is a prerequisite for the downstream deposition of histones to form chromatin. However, the coordinate regulation of these processes remains poorly understood. Here we demonstrate that Kap114p, the primary karyopherin/importin responsible for the nuclear import of histones H2A and H2B, modulates the deposition of histones
Nima, Mosammaparast +2 more
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Oncology, 2022 <b><i>Introduction:</i></b> Karyopherin alpha 2 (KPNA2) and karyopherin beta 1 (KPNB1) constitute nuclear transport protein complexes involved in nuclear import and are significant in tumor progression. Although high KPNA2 expression was associated with poor prognosis in solid tumors, the relationship between KPNA2 and KPNB1 ...
Yoshihito Ohhara +10 more
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A survey of the specificity and mechanism of 1,6 hexanediol-induced disruption of nuclear transport
Nucleus, 2023 Selective transport through the nuclear pore complex (NPC) depends on the dynamic binding of FG-repeat containing nucleoporins, the FG-nups, with each other and with Karyopherins (Kaps).
Elizabeth C. Riquelme Barrientos +4 more
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The binding site of karyopherin alpha for karyopherin beta overlaps with a nuclear localization sequence. [PDF]
Proceedings of the National Academy of Sciences, 1996 By using proteolysis, recombinant mutant proteins, or synthetic peptides and by testing these reagents in liquid phase binding or nuclear import assays, we have mapped binding regions of karyopherin alpha. We found that the C-terminal region of karyopherin alpha recognizes the nuclear localization sequence (NLS), whereas its N-terminal region binds ...
J, Moroianu, G, Blobel, A, Radu
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Structures of the Karyopherins Kap121p and Kap60p Bound to the Nuclear Pore-Targeting Domain of the SUMO Protease Ulp1p [PDF]
, 2017 The budding yeast small ubiquitin-like modifier (SUMO) protease Ulp1p catalyzes both the processing of newly synthesized SUMO to its mature form and the deconjugation of SUMO from target proteins, thereby regulating a wide range of cellular processes ...
Matsuura, Yoshiyuki +2 more
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The IMPORTance of the Nucleus during Flavivirus Replication
Viruses, 2017 Flaviviruses are a large group of arboviruses of significant medical concern worldwide. With outbreaks a common occurrence, the need for efficient viral control is required more than ever.
Adam J. Lopez-Denman, Jason M. Mackenzie
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A karyopherin acts in localized protein synthesis [PDF]
Cell Cycle, 2010 Multiple mechanisms are in place to regulate adequate synthesis of proteins, ranging from ways to ensure sequence fidelity, polypeptide folding and protein modification, to control of amounts and subcellular localization of the molecules. Some of these mechanisms act at the level of mRNA export and mRNA targeting.
Veenhoff, Liesbeth M. +2 more
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