Results 81 to 90 of about 56,140 (348)

Genetic alterations of Keap1 confers chemotherapeutic resistance through functional activation of Nrf2 and Notch pathway in head and neck squamous cell carcinoma

Cell Death and Disease, 2022
Keap1 mutations regulate Nrf2 activity and lead to chemoresistance in cancers. Yet the underlying molecular mechanisms of chemoresistance are poorly explored.
Syed S. Islam, Khawlah Qassem, Shafiqul Islam, Rashed R. Parag, Mohammed Z. Rahman, Walid A. Farhat, Herman Yeger, Abdelilah Aboussekhra, Bedri Karakas, Abu Shadat M. Noman   +9 more
doaj   +1 more source

Detection of thermal shift in cellular Keap1 by protein-protein interaction inhibitors using immunoblot- and fluorescence microplate-based assays

STAR Protocols, 2022
Summary: Pharmacologic inhibition of the protein-protein interaction (PPI) interface of the Keap1:Nrf2 complex, which leads to Nrf2 activation and cytoprotective gene expression, offers a promising strategy for disease prevention and treatment.
Sharadha Dayalan Naidu, Dina Dikovskaya, Terry W. Moore, Albena T. Dinkova-Kostova   +3 more
doaj   +1 more source

The KEAP1/NRF2 Signaling Pathway in Keratinization

Antioxidants, 2020
Keratinization is a tissue adaptation, but aberrant keratinization is associated with skin disorders such as ichthyoses, atopic dermatitis, psoriasis, and acne. The disease phenotype stems from the interaction between genes and the environment; therefore, an understanding of the adaptation machinery may lead to a new appreciation of pathomechanisms ...
Yosuke Ishitsuka, Tatsuya Ogawa, Dennis Roop   +2 more
openaire   +3 more sources

Modification of Keap1 Cysteine Residues by Sulforaphane [PDF]

Chemical Research in Toxicology, 2011
Activation of the transcription factor NF-E2-related factor-2 (Nrf2) through modification of Kelch-like ECH-associated protein 1 (Keap1) cysteines, leading to up-regulation of the antioxidant response element (ARE), is an important mechanism of cellular defense against reactive oxygen species and xenobiotic electrophiles.
Chenqi, Hu, Aimee L, Eggler, Andrew D, Mesecar, Richard B, van Breemen   +3 more
openaire   +2 more sources

Precision‐Engineered Silver Single‐Atom Carbon Dot Nanozymes for Theranostic Management of Acute Kidney Injury

Advanced Science, EarlyView.
Mitochondria‐targeted silver single‐atom carbon dot (T‐AgSA‐CDs) nanozymes enable dual SOD/GPx‐like ROS scavenging, real‐time imaging, and therapeutic effects for acute kidney injury (AKI). Atomically dispersed Ag sites synergize with carbon dots to restore renal redox hemostasis, protect mitochondrial function, mitigate inflammation, facilitate post ...
Tianle Tang, Jun Zhang, Yan Wang, Guoping Chen, Kehui Yuan, Yuan He, Chenchen Li, Sumaira Hanif, Yang Yang, Yanli Wang, Pir Muhammad   +10 more
wiley   +1 more source

Lung adenocarcinoma patients with KEAP1 mutation harboring low immune cell infiltration and low activity of immune environment

Thoracic Cancer, 2021
Background Kelch‐like ECH‐associated protein 1 (KEAP1) has been identified as a cancer driver gene in lung adenocarcinoma (LUAD), and increased evidence has given us clues about the association of KEAP1 mutation and immune characteristics.
Wanwan Cheng, Bin Xu, Haitao Zhang, Shencun Fang   +3 more
doaj   +1 more source

Physical and Functional Interaction of Sequestosome 1 with Keap1 Regulates the Keap1-Nrf2 Cell Defense Pathway [PDF]

Journal of Biological Chemistry, 2010
Nrf2 regulates the expression of numerous cytoprotective genes in mammalian cells. The activity of Nrf2 is regulated by the Cul3 adaptor Keap1, yet little is known regarding mechanisms of regulation of Keap1 itself. Here, we have used immunopurification of Keap1 and mass spectrometry, in addition to immunoblotting, to identify sequestosome 1 (SQSTM1 ...
Copple, Ian M., Lister, Adam, Obeng, Akua D., Kitteringham, Neil R., Jenkins, Rosalind E., Layfield, Robert, Foster, Brian J., Goldring, Christopher E., Park, B. Kevin   +8 more
openaire   +2 more sources

Immunoaffinity‐Mimetic Assembly of Peptide‐Aptamer Conjugates and Stem Cell‐Derived Exosomes into Hierarchical Microgels for Spinal Cord Injury Repair

Advanced Science, EarlyView.
Inspired by antibody‐antigen binding, this study develops an unprecedented immunoaffinity‐mimetic assembly strategy, with Peptide‐AptCD63 conjugates acting as antibody surrogates binding CD63 epitopes on mesenchymal stem cell‐derived exosomes. This creates a hierarchical microstructure intended to synergistically integrate antioxidative and anti ...
Dantong Zheng, Yuxin Bai, Zhibo Liu, Zinan Zhao, Yiming Kong, Yao Xu, Qi Jia, Rongrong Zhu, Yong Hu   +8 more
wiley   +1 more source

Agmatine Mitigates Acetaminophen-induced Acute Liver Injury in Mice: Involvement of HMGB1/RAGE/NFκB and Nrf2/HO-1 Signaling Pathways [PDF]

Journal of Applied Veterinary Sciences
An overdose of acetaminophen (APAP) is considered one of the primary sources of drug-induced liver injury. This study aimed to explore the mechanism of agmatine (Agma) against APAP-induced acute liver injury (ALI) in mice.
Ahmed Abdelhady, Mohammed Ayman, Marwa Abdelmageed   +2 more
doaj   +1 more source

Development of an enzyme-linked immunosorbent assay for Keap1-Nrf2 interaction inhibitors identification

Redox Biology, 2020
Development of Keap1–Nrf2 interaction inhibitors is a promising strategy for the discovery of therapeutic agents against oxidative stress-mediated diseases. Two motifs of Nrf2, ETGE and DLG motif, are responsible for Keap1-Nrf2 binding.
Yan Wang, Chu-Ying Xiao, Huang-Quan Lin, Jian-Shu Hu, Tsz-Ming Ip, David Chi-Cheong Wan   +5 more
doaj   +1 more source