Results 11 to 20 of about 15,338,333 (210)

Engineering Corynebacterium glutamicum for fast production of l-lysine and l-pipecolic acid

Applied Microbiology and Biotechnology, 2016
The Gram-positive Corynebacterium glutamicum is widely used for fermentative production of amino acids. The world production of L-lysine has surpassed 2 million tons per year. Glucose uptake and phosphorylation by C. glutamicum mainly occur by the phosphotransferase system (PTS) and to lesser extent by inositol permeases and glucokinases.
Perez, Fernando, Peters-Wendisch, Petra, Wendisch, Volker F.   +2 more
openaire   +3 more sources

Fermentative production of L‐pipecolic acid from glucose and alternative carbon sources

Biotechnology Journal, 2017
AbstractCorynebacterium glutamicum is used for the million‐ton scale production of amino acids and has recently been engineered for production of the cyclic non‐proteinogenic amino acid L‐pipecolic acid (L‐PA). In this synthetic pathway L‐lysine was converted to L‐PA by oxidative deamination, dehydration and reduction by L‐lysine 6‐dehydrogenase ...
Perez, Fernando, Risse, Joe Max, Friehs, Karl, Wendisch, Volker F.   +3 more
openaire   +3 more sources

R97 at “Handlebar” Binding Mode in Active Pocket Plays an Important Role in Fe(II)/α-Ketoglutaric Acid-Dependent Dioxygenase cis-P3H-Mediated Selective Synthesis of (2S,3R)-3-Hydroxypipecolic Acid

Molecules, 2023
Pipecolic acid (Pip) and its derivative hydroxypipecolic acids, such as (2S,3R)-3-hydroxypipecolic acid (cis-3-L-HyPip), are components of many natural and synthetic bioactive molecules. Fe(II)/α-ketoglutaric acid (Fe(II)/2-OG)-dependent dioxygenases can
Jiaojiao Guan, Yilei Lu, Zixuan Dai, Songyin Zhao, Yan Xu, Yao Nie   +5 more
doaj   +1 more source

Metabolomics of Mice Serum Analysis to Assess the Effects of Saikosaponin D on Breast Cancer [PDF]

Zhipu Xuebao, 2022
The implementation of targeted therapies for breast cancer has been a challenge. Saikosaponin D (SsD), a triterpene saponin derived from Bupleurum, exhibits potential therapeutic properties for cancer therapy.
ZHAO Ming-yue1, HOU Yu-zhu1, LIU Xin1, WANG Yang1, SUN Kai-ju1, PANG Bo2, LIU Shu-ying1, LI Dan1, SU Rui1,3   +8 more
doaj   +1 more source

Metabolic Profiling Identified a Novel Biomarker Panel for Metabolic Syndrome-Positive Hepatocellular Cancer

Frontiers in Endocrinology, 2022
Metabolic syndrome (MetS) is an independent risk factor for hepatocellular cancer (HCC). Currently, there is no highly sensitive and specific biomarkers for HCC surveillance in MetS population.
Lin-Lin Cao, Yi Han, Yuanxiao Wang, Lin Pei, Zhihong Yue, Li Qin, Boyu Liu, Jingwen Cui, Mei Jia, Hui Wang   +9 more
doaj   +1 more source

Changes of Metabolites in Acute Ischemic Stroke and Its Subtypes

Frontiers in Neuroscience, 2021
Existing techniques have many limitations in the diagnosis and classification of ischemic stroke (IS). Considering this, we used metabolomics to screen for potential biomarkers of IS and its subtypes and to explore the underlying related ...
Xin Wang, Xin Wang, Luyang Zhang, Luyang Zhang, Wenxian Sun, Wenxian Sun, Lu-lu Pei, Lu-lu Pei, Mengke Tian, Mengke Tian, Jing Liang, Jing Liang, Xinjing Liu, Xinjing Liu, Rui Zhang, Rui Zhang, Hui Fang, Hui Fang, Jun Wu, Jun Wu, Shilei Sun, Shilei Sun, Yuming Xu, Yuming Xu, Jian-Sheng Kang, Bo Song, Bo Song   +26 more
doaj   +1 more source

Proline and Proline Analogues Improve Development of Mouse Preimplantation Embryos by Protecting Them against Oxidative Stress

Cells, 2023
The culture of embryos in the non-essential amino acid L-proline (Pro) or its analogues pipecolic acid (PA) and L-4-thiazolidine carboxylic acid (L4T) improves embryo development, increasing the percentage that develop to the blastocyst stage and hatch ...
Madeleine L. M. Hardy, Dheerja Lakhiani, Michael B. Morris, Margot L. Day   +3 more
doaj   +1 more source

L-Pipecolic acid oxidase, a human enzyme essential for the degradation of L-pipecolic acid, is most similar to the monomeric sarcosine oxidases [PDF]

Biochemical Journal, 2000
L-Pipecolic acid oxidase activity is deficient in patients with peroxisome biogenesis disorders (PBDs). Because its role, if any, in these disorders is unknown, we cloned the associated human gene and expressed its protein product. The cDNA was cloned with the use of a reverse genetics approach based on the amino acid sequence obtained from purified L ...
G, Dodt, D G, Kim, S A, Reimann, B E, Reuber, K, McCabe, S J, Gould, S J, Mihalik   +6 more
openaire   +2 more sources

A Simple and Highly Sensitive HPLC Method with Fluorescent Detection for Determination of Pipecolic Acid in Mouse Brain Areas [PDF]

, 2012
A simple and highly sensitive high-performance liquid chromatography method for the determination of pipecolic acid in mouse brain areas was developed. After homogenization of brain and pretreatment with o-phthalaldehyde, the pipecolic acid and (2S,3S)-3-
9000270162760, 45337, 45106, 井上, 裕文, 徳毛, 孝至, 46145, 伊達, 有子, 45674, 9000005189599, 46146, 9000005174471, 9000006296797, 9000014625804, 45108, 阪田, 安彦, 田村, 豊, 小嶋, 英二朗, 9000014625915, 9000345371922, 9000004460018, 45371, 45373, 鶴田, 泰人, 西尾, 廣昭   +23 more
core   +2 more sources

Cell-free biocatalytic syntheses of L-pipecolic acid: a dual strategy approach and process intensification in flow [PDF]

, 2020
As an alternative to the traditional chemical synthesis or in-vivo production of L-Pipecolic acid, we have developed two ex-vivo strategies using purified and immobilised enzymes for the production of this key building block.
Paradisi, Francesca, Roura Padrosa, David, Calvey, Liam, Benítez-Mateos, Ana I.   +3 more
core   +2 more sources