Journal of Diabetes Research, 2020 Background. Paraoxonase 1 (PON1) is a calcium-dependent multifunctional enzyme that binds to high-density lipoproteins. The physiological function of PON1 is related to its lactonase activity.
Mi Zhou +6 more
doaj +1 more source
Expression of paraoxonase 1 in HEK293 cells and its effect on lactonase and arylesterase activity in samples containing human serum and/or prooxidant or antioxidant agents - a preliminary study [PDF]
Acta Poloniae Pharmaceutica, 2023 Expression of recombinant paraoxonase 1 (PON1) in the human embryonic kidney (HEK293F) cell line was evaluated. Two recombinant human PON1 proteins were investigated: PON1-Fc, which has an Fc-tag on its C-terminal, and His-PON1-Fc, which has an Fc-tag on
Anna Bizoń, Agnieszka Piwowar
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The Structure and Function of Paraoxonase-1 and Its Comparison to Paraoxonase-2 and -3
Molecules, 2020 Serum paraoxonase-1 (PON1) is the most studied member of the group of paraoxonases (PONs). This enzyme possesses three enzymatic activities: lactonase, arylesterase, and paraoxonase activity.
Ajda Taler-Verčič +2 more
doaj +1 more source
Human Paraoxonase-2 (PON2): Protein Functions and Modulation
Antioxidants, 2021 PON1, PON2, and PON3 belong to a family of lactone hydrolyzing enzymes endowed with various substrate specificities. Among PONs, PON2 shows the highest hydrolytic activity toward many acyl-homoserine lactones (acyl-HL) involved in bacterial quorum ...
Giuseppe Manco +2 more
doaj +1 more source
Toward Sustainability: Intensification of Light-Driven Whole Cell Biocatalysis. [PDF]
ChembiochemWhole‐cell, light‐driven biotransformations in photoautotrophic microorganisms offer the potential for higher atom economy by directly harnessing reducing equivalents and oxygen generated through natural photosynthesis. Current advances include the development of strains with improved growth rates and reactor designs that mitigate self‐shading ...
Malihan-Yap L +2 more
europepmc +2 more sources
Quorum-quenching activity of the AHL-lactonase from Bacillus licheniformis DAHB1 inhibits vibrio biofilm formation in vitro and reduces shrimp intestinal colonisation and mortality [PDF]
, 2014 Vibrio parahaemolyticus is a significant cause of gastroenteritis resulting from the consumption of undercooked sea foods and often cause significant infections in shrimp aquaculture. Vibrio virulence is associated with biofilm formation and is regulated
Shanthi, S. +4 more
core +3 more sources
Evaluation of a new high-throughput method for identifying quorum quenching bacteria [PDF]
, 2013 Quorum sensing (QS) is a population-dependent mechanism for bacteria to synchronize social behaviors such as secretion of virulence factors. The enzymatic interruption of QS, termed quorum quenching (QQ), has been suggested as a promising alternative ...
Bossier, Peter +6 more
core +2 more sources
Specificity and Enzyme Kinetics of the Quorum-quenching N-Acyl Homoserine Lactone Lactonase (AHL-lactonase) [PDF]
Journal of Biological Chemistry, 2004 N-Acyl homoserine lactone (AHL) quorum-sensing signals are the vital elements of bacterial quorum-sensing systems, which regulate diverse biological functions, including virulence. The AHL-lactonase, a quorumquenching enzyme encoded by aiiA from Bacillus sp., inactivates AHLs by hydrolyzing the lactone bond to produce corresponding N-acyl homoserines ...
Wang, L.-H. +3 more
openaire +2 more sources
Signal Disruption Leads to Changes in Bacterial Community Population
Frontiers in Microbiology, 2019 The disruption of bacterial signaling (quorum quenching) has been proven to be an innovative approach to influence the behavior of bacteria. In particular, lactonase enzymes that are capable of hydrolyzing the N-acyl homoserine lactone (AHL) molecules ...
Michael Schwab +15 more
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Cells, 2023 β-lactamase enzymes have generated significant interest due to their ability to confer resistance to the most commonly used family of antibiotics in human medicine.
Seydina M. Diene +8 more
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