Results 11 to 20 of about 102,219 (296)
LC3-associated phagocytosis [PDF]
Autophagy, 2014 A recent report from our group has described that upon engulfment of pathogens, a subset of phagosomes is formed to preserve antigens for prolonged presentation on MHC class II molecules. The distinctive feature of these particular vesicles is their coating with LC3/Atg8, a key component of the autophagy machinery. Here we discuss the possible outcomes
Romao Susana, Münz Christian
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Chromosomal mapping of the GFP-LC3 transgene in GFP-LC3 mice [PDF]
Autophagy, 2008 GFP-LC3 mice are widely used to monitor autophagy. Here, we report chromosomal mapping of the GFP.LC3 transgene in this mouse model. The transgene was located in a very distal region of chromosome 2, immediately upstream of a putative open reading frame that is thought to be a part of a pseudogene.
Akiko, Kuma, Noboru, Mizushima
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LC3 conjugation to lipid droplets
Autophagy, 2023 Macroautophagy/autophagy and lipid droplet (LD) biology are intricately linked, with autophagosome-dependent degradation of LDs in response to different signals. LDs play crucial roles in forming autophagosomes possibly by providing essential lipids and serving as a supportive autophagosome assembly platform at the endoplasmic reticulum (ER)-LD ...
Omrane, Mohyeddine +2 more
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LC3-Associated Phagocytosis and Inflammation [PDF]
Journal of Molecular Biology, 2017 LC3-associated phagocytosis (LAP) is a novel form of non-canonical autophagy where LC3 (microtubule-associated protein 1A/1B-light chain 3) is conjugated to phagosome membranes using a portion of the canonical autophagy machinery. The impact of LAP to immune regulation is best characterized in professional phagocytes, in particular macrophages, where ...
Bradlee L, Heckmann +4 more
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LC3-Associated Phagocytosis in Bacterial Infection
Pathogens, 2022 LC3-associated phagocytosis (LAP) is a noncanonical autophagy process reported in recent years and is one of the effective mechanisms of host defense against bacterial infection. During LAP, bacteria are recognized by pattern recognition receptors (PRRs), enter the body, and then recruit LC3 onto a single-membrane phagosome to form a LAPosome.
Jin Yuan +4 more
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Modified LC3 Dot Quantification Method
, 2022 Since the discovery of autophagy genes and proteins in the early1990s, numerous previously unknown physiological and pathological functions have been discovered for autophagy. At the same time, precise monitoring of autophagy has become important, and western blotting and fluorescence microscopy of the marker protein LC3 is widely used for this purpose.
Eskelinen Eeva-Liisa +2 more
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Macroautophagy without LC3 conjugation? [PDF]
Cell Research, 2016 A recent study makes the surprising observation that autophagosomes can still form in the absence of the core conjugation machinery. Furthermore, while such autophagosomes can fuse with lysosomes, their degradation is delayed, and this is associated with delayed destruction of the inner autophagosomal double membrane, highlighting a new role for ...
Renna, Maurizio, Rubinsztein, David C.
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Basal ryanodine receptor activity suppresses autophagic flux [PDF]
, 2017 The inositol 1,4,5-trisphosphate receptors (IP3Rs) and intracellular Ca2+ signaling are critically involved in regulating different steps of autophagy, a lysosomal degradation pathway.
Bannai, Hiroko +9 more
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Intestinal epithelial CGAS dampens inflammation by upregulating autophagy
Autophagy Reports, 2022 The pathogenesis of intestinal inflammation involves a complex network of cell signaling pathways. CGAS (cyclic GMP-AMP synthase) is a cytoplasmic DNA sensor that is most known for upregulating interferon-mediated inflammation.
Elizabeth A. Novak +5 more
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Evaluating Autophagy in Preimplantation Embryos
Autophagy Reports, 2022 Autophagy is an essential cellular degradation pathway in the mammalian preimplantation embryo. In this review, the process of autophagy is outlined and its function pertaining to preimplantation embryos is discussed.
Zuleika C. L. Leung +4 more
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