Results 1 to 10 of about 4,885 (120)

Interaction of Escherichia coli RecA Protein with LexA Repressor [PDF]

Journal of Biological Chemistry, 1996
The Escherichia coli RecA protein is involved in SOS induction, DNA repair, and homologous recombination. In vitro, RecA protein serves as a co-protease to cleave LexA repressor, the repressor of the SOS regulon; in addition, RecA protein promotes homologous pairing and DNA strand exchange, steps important to homologous recombination and DNA repair. To
Stephen Kowalczykowski
exaly   +5 more sources

The carboxy-terminal domain of the LexA repressor oligomerises essentially as the entire protein [PDF]

FEBS Letters, 1988
The ability of the isolated carboxy‐terminal domain of the LexA repressor of Escherichia coli to form dimers and tetramers has been investigated by equilibrium ultracentrifugation. This domain, that comprises the amino acids 85–202, is readily purified after self‐cleavage of the LexA repressor at alkaline pH.
M Schnarr, M Granger-Schnarr, J Pouyet   +2 more
exaly   +3 more sources

Purified lexA protein is a repressor of the recA and lexA genes. [PDF]

Proceedings of the National Academy of Sciences of the United States of America, 1981
Escherichia coli shows a pleiotropic response (the SOS response) to treatments that damage DNA or inhibit DNA replication. Previous evidence has suggested that the product of the lexA gene is involved in regulating the SOS response, perhaps as a repressor, and that it is sensitive to the recA protease.
David W Mount, J W Little
exaly   +3 more sources

DdiA, an XRE family transcriptional regulator, is a co-regulator of the DNA damage response in Myxococcus xanthus [PDF]

Journal of Bacteriology
Repair of DNA damage is essential for genome integrity. DNA damage elicits a DNA damage response (DDR) that includes error-free and error-prone, i.e., mutagenic, repair. The SOS response is a widely conserved system in bacteria that regulates the DDR and
Jana Jung, Timo Glatter, Marco Herfurth, Lotte Søgaard-Andersen   +3 more
doaj   +2 more sources

LexA, an SOS response repressor, activates TGase synthesis in Streptomyces mobaraensis

Frontiers in Microbiology
Transglutaminase (EC 2.3.2.13, TGase), an enzyme that catalyzes the formation of covalent cross-links between protein or peptide molecules, plays a critical role in commercial food processing, medicine, and textiles.
Xinyu Shi, Hao Yan, Fang Yuan, Fang Yuan, Guoying Li, Jingfang Liu, Chunli Li, Xiaobin Yu, Zilong Li, Yunping Zhu, Weishan Wang, Weishan Wang   +11 more
doaj   +3 more sources

Roles of Alp and Prt Regulons in the Response of Pseudomonas aeruginosa to UV-C Light. [PDF]

Environ Microbiol Rep
Our results show that LexA‐regulated genes are the first to be induced after DNA damage, while autolysis functions controlled by the Alp system are the last. Death by UV is mediated primarily by Alp or Prt systems, depending on the Pseudomonas aeruginosa subline. ABSTRACT UV light is a well‐studied environmental DNA damaging agent.
Fonseca MRB, Oliveira RS, Kustor H, Freitas RRL, Mello EBS, Alvarez-Martinez CE, Galhardo RS.   +6 more
europepmc   +2 more sources

Selection, counterselection, and conditional gene expression for genetic biocontrol of insects. [PDF]

Insect Sci
Selection, counterselection, and conditional expression can be used for sex separation. Selection (S) and counterselection (CS) genes are linked to the sex chromosomes (e.g., the X and Y chromosomes) to confer sex‐specific selection or counterselection based on the presence or absence of the S or CS genes.
Hempel M, Tu Z.
europepmc   +2 more sources

Integration of molecular modelling and in vitro studies to inhibit LexA proteolysis

Frontiers in Cellular and Infection Microbiology, 2023
IntroductionAs antibiotic resistance has become more prevalent, the social and economic impacts are increasingly pressing. Indeed, bacteria have developed the SOS response which facilitates the evolution of resistance under genotoxic stress.
Zachariah P. Schuurs, Zachariah P. Schuurs, John P. McDonald, Laura V. Croft, Derek J. Richard, Roger Woodgate, Neha S. Gandhi, Neha S. Gandhi   +7 more
doaj   +1 more source

"Activated"-RecA protein affinity chromatography of LexA repressor and other SOS-regulated proteins. [PDF]

Proceedings of the National Academy of Sciences, 1989
We have developed an affinity column to study the interaction of LexA repressor and other substrates with the activated form of RecA protein. Nucleoprotein complexes of RecA protein, (dT)25-30, and adenosine 5'-[gamma-S]thio-triphosphate were formed in solution and bound to RecA protein-agarose columns.
N, Freitag, K, McEntee
openaire   +2 more sources

The Zinc Finger Transcription Factor Fts2 Represses the Yeast-to-Filament Transition in the Dimorphic Yeast Yarrowia lipolytica

mSphere, 2022
The yeast-to-filament transition is an important cellular response to environmental stimulations in dimorphic fungi. In addition to activators, there are repressors in the cells to prevent filament formation, which is important to keep the cells in the ...
Jia-Wen Chen, Yi-Sheng Mao, Lv-Qiao Yan, Xiang-Dong Gao   +3 more
doaj   +1 more source