Molecular tension microscopy of the LINC complex in live cells [PDF]
STAR Protocols, 2022 Summary: We present a protocol to measure the effect of pharmacological treatments on the mechanical tension experienced by nesprins at the cytoplasmic surface of the nuclear envelope of mammalian cells in culture.
François Sipieter +3 more
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The LINC complex, mechanotransduction, and mesenchymal stem cell function and fate [PDF]
Journal of Biological Engineering, 2019 Mesenchymal stem cells (MSCs) show tremendous promise as a cell source for tissue engineering and regenerative medicine, and are understood to be mechanosensitive to external mechanical environments. In recent years, increasing evidence points to nuclear
Tasneem Bouzid +7 more
doaj +5 more sources
SUN/KASH interactions facilitate force transmission across the nuclear envelope [PDF]
Nucleus, 2019 LINC complexes (Linker of Nucleoskeleton and Cytoskeleton), consisting of inner nuclear membrane SUN (Sad1, UNC-84) proteins and outer nuclear membrane KASH (Klarsicht, ANC-1, and Syne Homology) proteins, are essential for nuclear positioning, cell ...
Hongyan Hao, Daniel A. Starr
doaj +2 more sources
Prrx1-driven LINC complex disruption in vivo reduces osteoid deposition but not bone quality after voluntary wheel running. [PDF]
PLoS ONEThe Linker of Nucleoskeleton and Cytoskeleton (LINC) complex serves to connect the nuclear envelope and the cytoskeleton, influencing cellular processes such as nuclear arrangement, architecture, and mechanotransduction.
Scott Birks +9 more
doaj +2 more sources
Long non-coding RNA Linc-RAM enhances myogenic differentiation by interacting with MyoD
Nature Communications, 2017 Long non-coding RNAs (lncRNAs) are important regulators of diverse biological processes. Here we report on functional identification and characterization of a novel long intergenic non-coding RNA with MyoD-regulated and skeletal muscle-restricted ...
Yong Zhang, Jiao Meng, Antony K Chen
exaly +2 more sources
The mammalian LINC complex component SUN1 regulates muscle regeneration by modulating drosha activity [PDF]
eLife, 2019 Here we show that a major muscle specific isoform of the murine LINC complex protein SUN1 is required for efficient muscle regeneration. The nucleoplasmic domain of the isoform specifically binds to and inhibits Drosha, a key component of the ...
Tsui Han Loo +6 more
doaj +2 more sources
SWR1-Independent Association of H2A.Z to the LINC Complex Promotes Meiotic Chromosome Motion [PDF]
Frontiers in Cell and Developmental Biology, 2020 The H2A.Z histone variant is deposited into the chromatin by the SWR1 complex, affecting multiple aspects of meiosis. We describe here a SWR1-independent localization of H2A.Z at meiotic telomeres and the centrosome. We demonstrate that H2A.Z colocalizes
Sara González-Arranz +10 more
doaj +2 more sources
LINC-complex mediated positioning of the vegetative nucleus is involved in calcium and ROS signaling in Arabidopsis pollen tubes [PDF]
Nucleus, 2020 Nuclear movement and positioning play a role in developmental processes throughout life. Nuclear movement and positioning are mediated primarily by linker of nucleoskeleton and cytoskeleton (LINC) complexes.
Morgan Moser +3 more
doaj +2 more sources
Role of KASH domain lengths in the regulation of LINC complexes. [PDF]
Molecular Biology of the Cell, 2019 The linker of the nucleoskeleton and cytoskeleton (LINC) complex is formed by the conserved interactions between Sad-1 and UNC-84 (SUN) and Klarsicht, ANC-1, SYNE homology (KASH) domain proteins, providing a physical coupling between the nucleoskeleton ...
Fadavi, Darya +10 more
core +6 more sources
Nuclear envelopathies: a complex LINC between nuclear envelope and pathology [PDF]
Orphanet Journal of Rare Diseases, 2017 Since the identification of the first disease causing mutation in the gene coding for emerin, a transmembrane protein of the inner nuclear membrane, hundreds of mutations and variants have been found in genes encoding for nuclear envelope components ...
Alexandre Janin +4 more
doaj +4 more sources