iLIR database: A web resource for LIR motif-containing proteins in eukaryotes. [PDF]
Autophagy, 2016 Atg8-family proteins are the best-studied proteins of the core autophagic machinery. They are essential for the elongation and closure of the phagophore into a proper autophagosome. Moreover, Atg8-family proteins are associated with the phagophore from the initiation of the autophagic process to, or just prior to, the fusion between autophagosomes with
Jacomin AC +3 more
europepmc +8 more sources
iLIR@viral: A web resource for LIR motif-containing proteins in viruses. [PDF]
Autophagy, 2017 Macroautophagy/autophagy has been shown to mediate the selective lysosomal degradation of pathogenic bacteria and viruses (xenophagy), and to contribute to the activation of innate and adaptative immune responses. Autophagy can serve as an antiviral defense mechanism but also as a proviral process during infection.
Jacomin AC +3 more
europepmc +6 more sources
An atypical LIR motif within UBA5 (ubiquitin like modifier activating enzyme 5) interacts with GABARAP proteins and mediates membrane localization of UBA5. [PDF]
Autophagy, 2020 Short linear motifs, known as LC3-interacting regions (LIRs), interact with mactoautophagy/autophagy modifiers (Atg8/LC3/GABARAP proteins) via a conserved universal mechanism. Typically, this includes the occupancy of 2 hydrophobic pockets on the surface of Atg8-family proteins by 2 specific aromatic and hydrophobic residues within the LIR motifs. Here,
Huber J +11 more
europepmc +7 more sources
ATG4B contains a C-terminal LIR motif important for binding and efficient cleavage of mammalian orthologs of yeast Atg8. [PDF]
Autophagy, 2017 The cysteine protease ATG4B cleaves off one or more C-terminal residues of the inactive proform of proteins of the ortholog and paralog LC3 and GABARAP subfamilies of yeast Atg8 to expose a C-terminal glycine that is conjugated to phosphatidylethanolamine during autophagosome formation.
Skytte Rasmussen M +11 more
europepmc +6 more sources
Atg8 family proteins, LIR/AIM motifs and other interaction modes
Autophagy Reports, 2023 The Atg8 family of ubiquitin-like proteins play pivotal roles in autophagy and other processes involving vesicle fusion and transport where the lysosome/vacuole is the end station. Nuclear roles of Atg8 proteins are also emerging.
Vladimir V. Rogov +16 more
doaj +5 more sources
Selective Autophagy: ATG8 Family Proteins, LIR Motifs and Cargo Receptors
Journal of Molecular Biology, 2020 Selective autophagy relies on soluble or membrane-bound cargo receptors that recognize cargo and bring about autophagosome formation at the cargo. The cargo-bound receptors interact with lipidated ATG8 family proteins anchored in the membrane at the concave side of the forming autophagosome.
Johansen, Terje, Lamark, Trond
openaire +5 more sources
FYCO1 Contains a C-terminally Extended, LC3A/B-preferring LC3-interacting Region (LIR) Motif Required for Efficient Maturation of Autophagosomes during Basal Autophagy. [PDF]
J Biol Chem, 2015 FYCO1 (FYVE and coiled-coil protein 1) is a transport adaptor that binds to phosphatidylinositol 3-phosphate, to Rab7, and to LC3 (microtubule-associated protein 1 light chain 3) to mediate transport of late endosomes and autophagosomes along microtubules in the plus end direction.
Olsvik HL +7 more
europepmc +7 more sources
LC3C, bound selectively by a noncanonical LIR motif in NDP52, is required for antibacterial autophagy. [PDF]
Mol Cell, 2012 Autophagy protects cellular homeostasis by capturing cytosolic components and invading pathogens for lysosomal degradation. Autophagy receptors target cargo to autophagy by binding ATG8 on autophagosomal membranes. The expansion of the ATG8 family in higher eukaryotes suggests that specific interactions with autophagy receptors facilitate differential ...
von Muhlinen N +8 more
europepmc +5 more sources
Analysis of the native conformation of the LIR/AIM motif in the Atg8/LC3/GABARAP-binding proteins [PDF]
Autophagy, 2015 The Atg8/LC3/GABARAP family of proteins, a group that has structural homology with ubiquitin, connects with a large set of binding partners to function in macroautophagy (hereafter autophagy). This interaction occurs primarily via a conserved motif termed the LC3-interacting region (LIR), or the Atg8-interacting motif (AIM).
Hana, Popelka, Daniel J, Klionsky
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Members of the autophagy class III phosphatidylinositol 3-kinase complex I interact with GABARAP and GABARAPL1 via LIR motifs [PDF]
Autophagy, 2019 Autophagosome formation depends on a carefully orchestrated interplay between membrane-associated protein complexes. Initiation of macroautophagy/autophagy is mediated by the ULK1 (unc-51 like autophagy activating kinase 1) protein kinase complex and the autophagy-specific class III phosphatidylinositol 3-kinase complex I (PtdIns3K-C1).
Åsa Birna Birgisdottir +11 more
openaire +6 more sources