Results 1 to 10 of about 320 (99)

Luciferase‐free Luciferin Electrochemiluminescence

open access: yesAngewandte Chemie International Edition, 2022
AbstractLuciferin is one of Nature's most widespread luminophores, and enzymes that catalyze luciferin luminescence are the basis of successful commercial “glow” assays for gene expression and metabolic ATP formation. Herein we report an electrochemical method to promote firefly's luciferin luminescence in the absence of its natural biocatalyst ...
Mattia Belotti   +7 more
openaire   +4 more sources

Biosynthesis of firefly luciferin in adult lantern: decarboxylation of L-cysteine is a key step for benzothiazole ring formation in firefly luciferin synthesis. [PDF]

open access: yesPLoS ONE, 2013
Bioluminescence in fireflies and click beetles is produced by a luciferase-luciferin reaction. The luminescence property and protein structure of firefly luciferase have been investigated, and its cDNA has been used for various assay systems.
Yuichi Oba   +4 more
doaj   +1 more source

Biosensing firefly luciferin synthesis in bacteria reveals a cysteine-dependent quinone detoxification route in Coleoptera

open access: yesScientific Reports, 2022
Luciferin biosynthetic origin and alternative biological functions during the evolution of beetles remain unknown. We have set up a bioluminescent sensing method for luciferin synthesis from cysteine and benzoquinone using E.
Daniel Rangel de Souza   +3 more
doaj   +1 more source

Sustained Effect of Hyaluronic Acid in Subcutaneous Administration to the Cochlear Spiral Ganglion. [PDF]

open access: yesPLoS ONE, 2016
The spatiotemporal distribution of drugs in the inner ear cannot be precisely evaluated because of its small area and complex structure. In the present study, we used hyaluronic acid (HA)-dispersed luciferin to image transgenic mice and to determine the ...
Yozo Inagaki   +11 more
doaj   +1 more source

New luciferin-based probe substrates for human CYP26A1

open access: yesBiochemistry and Biophysics Reports, 2020
Activity of human CYP26A1 towards six proluciferin probe substrates and their ester derivatives was monitored. These included three monofluorobenzyl ether isomers and three five-membered heterocycles.
Shishir Sharma   +5 more
doaj   +1 more source

Chemistry in Fungal Bioluminescence: Theoretical Studies on Biosynthesis of Luciferin from Caffeic Acid and Regeneration of Caffeic Acid from Oxidized Luciferin

open access: yesJournal of Fungi, 2023
Fungal bioluminescence is widely distributed in the terrestrial environment. At a specific stage of growth, luminescent fungi shine green light at the fruiting body or mycelium.
Xiayu Liu, Mingyu Wang, Yajun Liu
doaj   +1 more source

Transcriptome analysis reveals candidate genes involved in luciferin metabolism in Luciola aquatilis (Coleoptera: Lampyridae) [PDF]

open access: yesPeerJ, 2016
Bioluminescence, which living organisms such as fireflies emit light, has been studied extensively for over half a century. This intriguing reaction, having its origins in nature where glowing insects can signal things such as attraction or defense, is ...
Wanwipa Vongsangnak   +2 more
doaj   +2 more sources

Effects of pentoxifylline on canine platelet aggregation

open access: yesVeterinary Medicine and Science, 2021
Background Pentoxifylline can decrease platelet function in humans, but the anti‐platelet effects of pentoxifylline in dogs is unknown. The addition of a luciferin–luciferase reagent during platelet aggregometry can induce a dose‐dependent potentiation ...
John M. Thomason   +3 more
doaj   +1 more source

Bioluminescent Sensors for Ca++ Flux Imaging and the Introduction of a New Intensity-Based Ca++ Sensor

open access: yesFrontiers in Bioengineering and Biotechnology, 2021
Sensitive detection of biological events is a goal for the design and characterization of sensors that can be used in vitro and in vivo. One important second messenger is Ca++ which has been a focus of using genetically encoded Ca++ indicators (GECIs ...
Jie Yang, Carl Hirschie Johnson
doaj   +1 more source

Label-free phenotypic profiling identified D-luciferin as a GPR35 agonist. [PDF]

open access: yesPLoS ONE, 2012
Fluorescent and luminescent probes are essential to both in vitro molecular assays and in vivo imaging techniques, and have been extensively used to measure biological function.
Haibei Hu, Huayun Deng, Ye Fang
doaj   +1 more source

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