Results 21 to 30 of about 419 (198)

Influence of Bioluminescence Imaging Dynamics by D-Luciferin Uptake and Efflux Mechanisms

Molecular Imaging, 2012
Bioluminescence imaging (BLI) detects light generated by luciferase-mediated oxidation of substrate and is used widely for evaluating transgene expression in cell-based assays and in vivo in relevant preclinical models.
Yimao Zhang, Mrudula Pullambhatla, John Laterra, Martin G. Pomper   +3 more
doaj   +1 more source

First Record of Bioluminescence in a Sipunculan Worm

Frontiers in Marine Science, 2021
During a search for bioluminescent marine annelids around the island of Carrie Bow Cay, Belize, we discovered bioluminescence in the sipunculan worm Nephasoma pellucidum. The identification of N. pellucidum was verified by DNA barcoding of ∼650 bp of the
Anderson G. Oliveira, Danilo T. Amaral, Mary Colleen Hannon, Anja Schulze   +3 more
doaj   +1 more source

Genomic and experimental data provide new insights into luciferin biosynthesis and bioluminescence evolution in fireflies

Scientific Reports, 2020
Fireflies are among the most charismatic insects for their spectacular bioluminescence, but the origin and evolution of bioluminescence remain elusive.
Ru Zhang, Jinwu He, Zhiwei Dong, Guichun Liu, Yuan Yin, Xinying Zhang, Qi Li, Yandong Ren, Yongzhi Yang, Wei Liu, Xianqing Chen, Wenhao Xia, Kang Duan, Fei Hao, Zeshan Lin, Jie Yang, Zhou Chang, Ruoping Zhao, Wenting Wan, Sihan Lu, Yanqiong Peng, Siqin Ge, Wen Wang, Xueyan Li   +23 more
doaj   +1 more source

Expression of a multigene mushroom luciferin biosynthesis pathway as a pseudo-polycistron in plants

Scientific Reports
Mushroom bioluminescence is based on a luciferin/luciferase cycle that includes four catalytic enzymes and a post-translational modifier phosphopantetheinyl-transferase (NpgA).
David Samson, Natalie S. Thompson, Vijay R. Sheri, Sairam V. Rudrabhatla, Wayne R. Curtis   +4 more
doaj   +1 more source

Orthogonal Bioluminescent Probes from Disubstituted Luciferins

Biochemistry, 2021
Bioluminescence imaging with luciferase-luciferin pairs is routinely used to monitor cellular functions. Multiple targets can be visualized in tandem using luciferases that process unique substrates, but only a handful of such orthogonal probes are known. Multiplexed studies require additional robust, light-emitting molecules.
Sierra J. Williams, Clare S. Hwang, Jennifer A. Prescher   +2 more
openaire   +5 more sources

Synthetic Nanobiology Actuated Lipometabolic Cell Factory for Autologous Tumor Immunotherapy

Advanced Functional Materials, EarlyView.
FA plays a crucial role in the interaction between tumor cells and the tumor microenvironment, especially for the immune response. A biocatalytic immunoenhancement strategy is developed to boost antitumor immunity by FA metabolic orientation to ceramide. Through the design of this delicate catalytic immunoenhancement strategy, the synthetic nanobiology
Shoujie Zhao, Yejing Zhu, Hengchao Yu, Jun Zhu, Bo Wang, Yu Qiao, Zhimin Tian, Junlong Zhao, Lei Liu   +8 more
wiley   +1 more source

Successful delivery of viviparous lantern shark from an artificial uterus and the self-production of lantern shark luciferin.

PLoS ONE, 2023
Our recent success in the long-term maintenance of lantern shark embryos in artificial uterine systems has provided a novel option for the medical treatment of premature embryos for captive viviparous elasmobranchs.
Taketeru Tomita, Minoru Toda, Atsushi Kaneko, Kiyomi Murakumo, Kei Miyamoto, Keiichi Sato   +5 more
doaj   +1 more source

RNA‐Micelles as Self‐Assembling Structures for Efficient Co‐Delivery of Synergistic siRNA and Nucleoside Analogues to Treat CRC Lung Metastasis

Advanced Functional Materials, EarlyView.
Two kinds of self‐assembled RNA micelles were used to co‐deliver synergistic siRNA and nucleoside analogues for the treatment of colorectal cancer lung metastases. Near‐complete elimination of lung cancer metastases was confirmed in an orthotopic lung metastasis model constructed using human colorectal cancer lung metastases patient surgical samples to
Kai Jin, Piotr Rychahou, Daniel W. Binzel, B. Mark Evers, Peixuan Guo   +4 more
wiley   +1 more source

Optogenetic inhibition of neurons by internal light production

Frontiers in Behavioral Neuroscience, 2014
Optogenetics is an extremely powerful tool for selective neuronal activation/inhibition and dissection of neural circuits. However, a limitation of in vivo optogenetics is that an animal must be tethered to an optical fiber for delivery of light.
Benjamin eLand, Catherine eBrayton, Kara eFurman, Zoe eLaPalombara, Ralph eDiLeone   +4 more
doaj   +1 more source

5,5-Dialkylluciferins are thermal stable substrates for bioluminescence-based detection systems.

PLoS ONE, 2020
Firefly luciferase-based ATP detection assays are frequently used as a sensitive, cost-efficient method for monitoring hygiene in many industrial settings.
Ce Shi, Michael P Killoran, Mary P Hall, Paul Otto, Monika G Wood, Ethan Strauss, Lance P Encell, Thomas Machleidt, Keith V Wood, Thomas A Kirkland   +9 more
doaj   +1 more source