Results 21 to 30 of about 76,328 (190)

A Method to Determine Lysine Acetylation Stoichiometries [PDF]

International Journal of Proteomics, 2014
Lysine acetylation is a common protein posttranslational modification that regulates a variety of biological processes. A major bottleneck to fully understanding the functional aspects of lysine acetylation is the difficulty in measuring the proportion of lysine residues that are acetylated.
Nakayasu, Ernesto S., Wu, Si, Sydor, Michael A., Shukla, Anil K., Weitz, Karl K., Moore, Ronald J., Hixson, Kim K., Kim, Jong-Seo, Petyuk, Vladislav A., Monroe, Matthew E., Pasa-Tolic, Ljiljiana, Qian, Wei-Jun, Smith, Richard D., Adkins, Joshua N., Ansong, Charles   +14 more
openaire   +2 more sources

Stimulation of Fibronectin Matrix Assembly by Lysine Acetylation [PDF]

Cells, 2020
Diabetic nephropathy, a devastating consequence of diabetes mellitus, is characterized by the accumulation of extracellular matrix (ECM) that disrupts the kidney’s filtration apparatus. Elevated glucose levels increase the deposition of a fibronectin (FN) matrix by mesangial cells, the primary matrix-producing cells of the kidney, and also increase ...
Maria E. Vega, Birgit Kastberger, Bernhard Wehrle-Haller, Jean E. Schwarzbauer   +3 more
openaire   +4 more sources

Enhancement of lysine acetylation accelerates wound repair [PDF]

Communicative & Integrative Biology, 2013
In physiopathological conditions, such as diabetes, wound healing is significantly compromised and chronic complications, including ulcers, may occur. In a mouse model of skin repair, we recently reported that wound treatment with Sirtuin activators and class I HDAC inhibitors induced keratinocyte proliferation and enhanced healing via a nitric oxide ...
Spallotta Francesco, Spallotta Francesco, Cencioni Chiara, Cencioni Chiara, Straino Stefania, Sbardella Gianluca, Castellano Sabrina, Capogrossi Maurizio C, Martelli Fabio, Gaetano Carlo   +9 more
openaire   +4 more sources

Lysine acetylation of major Chlamydia trachomatis antigens [PDF]

EuPA Open Proteomics, 2016
Chlamydia trachomatis (Ct) is a human pathogen causing trachoma and infertility. We investigated acetylation at lysine residues of chlamydial antigenic proteins: major outer membrane protein (MOMP), 60 kDa chaperonin (chlamydial Hsp60), elongation factor G (EF-G), enolase and the polymorphic membrane proteins PmpB, PmpE and PmpF.
Mihailovic, Jelena, Inic-Kanada, Aleksandra, Smiljanic, Katarina, Stein, Elisabeth, Barisani-Asenbauer, Talin, Cirkovic Velickovic, Tanja   +5 more
openaire   +2 more sources

Quantitative Profiling of Lysine Acetylation Reveals Dynamic Crosstalk between Receptor Tyrosine Kinases and Lysine Acetylation

PLOS ONE, 2015
Lysine acetylation has been primarily investigated in the context of transcriptional regulation, but a role for acetylation in mediating other cellular responses has emerged. Multiple studies have described global lysine acetylation profiles for particular biological states, but none to date have investigated the temporal dynamics regulating cellular ...
Bryson, Bryan D., White, Forest M.
openaire   +6 more sources

Site-Specific Reactivity of Nonenzymatic Lysine Acetylation [PDF]

ACS Chemical Biology, 2015
Protein acetylation of lysine ε-amino groups is abundant in cells, particularly within mitochondria. The contribution of enzyme-catalyzed and nonenzymatic acetylation in mitochondria remains unresolved. Here, we utilize a newly developed approach to measure site-specific, nonenzymatic acetylation rates for 90 sites in eight native purified proteins ...
Baeza, Josue, Smallegan, Michael J., Denu, John M.   +2 more
openaire   +2 more sources

Lysine acetylation regulates the activity of nuclear Pif1 [PDF]

Journal of Biological Chemistry, 2020
In Saccharomyces cerevisiae, the Pif1 helicase functions in both nuclear and mitochondrial DNA replication and repair processes, preferentially unwinding RNA:DNA hybrids and resolving G-quadruplex structures. We sought to determine how the various activities of Pif1 are regulated in vivo Here, we report lysine acetylation of nuclear Pif1 and ...
Onyekachi E. Ononye, Christopher W. Sausen, Lata Balakrishnan, Matthew L. Bochman   +3 more
openaire   +2 more sources

Studying the Lysine Acetylation of Malate Dehydrogenase [PDF]

Journal of Molecular Biology, 2017
Protein acetylation plays important roles in many biological processes. Malate dehydrogenase (MDH), a key enzyme in the tricarboxylic acid cycle, has been identified to be acetylated in bacteria by proteomic studies, but no further characterization has been reported. One challenge for studying protein acetylation is to get purely acetylated proteins at
Sumana, Venkat, Caroline, Gregory, Jourdan, Sturges, Qinglei, Gan, Chenguang, Fan   +4 more
openaire   +2 more sources

Histone H4 lysine 16 acetylation regulates cellular lifespan [PDF]

Nature, 2009
Cells undergoing developmental processes are characterized by persistent non-genetic alterations in chromatin, termed epigenetic changes, represented by distinct patterns of DNA methylation and histone post-translational modifications. Sirtuins, a group of conserved NAD(+)-dependent deacetylases or ADP-ribosyltransferases, promote longevity in diverse ...
Dang, Weiwei, Steffen, Kristan K., Perry, Rocco, Dorsey, Jean A., Johnson, F. Brad, Shilatifard, Ali, Kaeberlein, Matt, Kennedy, Brian K., Berger, Shelley L.   +8 more
openaire   +2 more sources

PICALM::MLLT10 translocated leukemia

FEBS Letters, EarlyView.
This comprehensive review of PICALM::MLLT10 translocated acute leukemia provides an in‐depth review of the structure and function of CALM, AF10, and the fusion oncoprotein (1). The multifaceted molecular mechanisms of oncogenesis, including nucleocytoplasmic shuttling (2), epigenetic modifications (3), and disruption of endocytosis (4), are then ...
John M. Cullen, Antonia C. Nakatsugawa, Natalie Barton, Henry Haines, Gary S. Stein, Janet L. Stein, Daniel S. Wechsler, Jessica L. Heath   +7 more
wiley   +1 more source