Results 11 to 20 of about 2,779 (193)

Development of reverse genetic tools to study Chapare and Machupo viruses [PDF]

Virology, 2023
Arenaviruses are highly pathogenic viruses that pose a serious public health threat. Chapare virus (CHAV) and Machupo virus (MACV), two New World arenaviruses, cause hemorrhagic fevers with case fatality rates of up to 45%. Research on therapeutic drug targets and vaccines for these viruses is limited because biosafety level 4 containment is required ...
Michael Flint, Joel M Montgomery, César G Albariño   +2 more
exaly   +4 more sources

Family-specific degenerate primer design: a tool to design consensus degenerated oligonucleotides [PDF]

Biotechnology Research International, Volume 2013, Issue 1, 2013., 2013
Designing degenerate PCR primers for templates of unknown nucleotide sequence may be a very difficult task. In this paper, we present a new method to design degenerate primers, implemented in family-specific degenerate primer design (FAS-DPD) computer ...
Borio, Cristina Silvia, Ghiringhelli, Pablo Daniel, Goñi, Sandra Elizabeth, Iserte, Javier Alonso, Lozano, Mario Enrique, Stephan, Betina Inés   +5 more
core   +4 more sources

Tuning the binding interface between Machupo virus glycoprotein and human transferrin receptor [PDF]

Proteins: Structure, Function, and Bioinformatics, 2020
AbstractMachupo virus, known to cause hemorrhagic fevers, enters human cells via binding with its envelope glycoprotein to transferrin receptor 1 (TfR). Similarly, the receptor interactions have been explored in biotechnological applications as a molecular system to ferry therapeutics across the cellular membranes and through the impenetrable blood ...
Dick J Sjöström, Anneli Lundgren, Scott J Garforth, Sinisa Bjelic   +3 more
openaire   +5 more sources

Structure of Machupo virus polymerase in complex with matrix protein Z [PDF]

Nature Communications, 2021
The RNA polymerase L of arenaviruses is of interest for drug design and its activity is inhibited by the matrix protein Z. Here, the authors present the cryo-EM structure of the Machupo virus polymerase L in complex with matrix protein Z and discuss the ...
Jun Ma, Shuangyue Zhang, Xinzheng Zhang
doaj   +3 more sources

A STAT-1 knockout mouse model for Machupo virus pathogenesis [PDF]

Virology Journal, 2011
Background Machupo virus (MACV), a member of the Arenaviridae, causes Bolivian hemorrhagic fever, with ~20% lethality in humans. The pathogenesis of MACV infection is poorly understood, and there are no clinically proven treatments for disease.
Shurtleff Amy C, Stuthman Kelly S, Bradfute Steven B, Bavari Sina   +3 more
doaj   +3 more sources

Development and characterization of a reverse genetics system for the lineage II Chicava strain of Machupo virus in a guinea pig model. [PDF]

PLoS Neglected Tropical Diseases
BackgroundMachupo virus (MACV) is a New World mammarenavirus (hereafter referred to as "arenavirus") and the etiologic agent of Bolivian hemorrhagic fever (BHF).
Emily Mantlo, Junki Maruyama, John T Manning, Rachel A Reyna, Cheng Huang, Slobodan Paessler   +5 more
doaj   +2 more sources

A review of emerging health threats from zoonotic New World mammarenaviruses [PDF]

BMC Microbiology
Despite repeated spillover transmission and their potential to cause significant morbidity and mortality in human hosts, the New World mammarenaviruses remain largely understudied.
Arianna Lendino, Adrian A. Castellanos, David M. Pigott, Barbara A. Han   +3 more
doaj   +2 more sources

Animal Models of Pathogenic New World Arenaviruses [PDF]

Microorganisms
Since the emergence of Junín virus in 1953, pathogenic New World arenaviruses have remained a public health concern. These viruses, which also include Machupo virus, Guanarito virus, Sabiá virus, and Chapare virus, cause acute viral hemorrhagic fever and
Alexander V. Alvarado, Robert W. Cross, Thomas W. Geisbert, Courtney Woolsey   +3 more
doaj   +2 more sources

Validation of Сhoice of Laboratory Model for Preclinical Estimation of Medical Protectors Against Bolivian Hemorrhagic Fever

Вестник войск РХБ защиты, 2023
This review is dedicated to the peculiarities of pathogenesis of the experimental Bolivian hemorrhagic fever (BHF) - the disease, caused by Machupo virus (Arenaviridae family).
T. E. Sizikova, V. N. Lebedev, V. B. Pantukhov, E. Y. Vakhnov, S. V. Borisevich   +4 more
doaj   +1 more source

Ribonucleoprotein transport in Negative Strand RNA viruses

Biology of the Cell, Volume 115, Issue 1, January 2023., 2023
The genome replication of Negative‐sense, single‐stranded RNA viruses most‐often segregate in membrane‐less environments called inclusion bodies (IBs). These “organelles” usually locate far from the cell surface from where new virions are released. Here, for each viral family, we discuss how the genome progeny is transported from the IBs to reach the ...
Cédric Diot, Gina Cosentino, Marie‐Anne Rameix‐Welti   +2 more
wiley   +1 more source