Results 121 to 130 of about 6,252 (250)

Application of Metagenomic and Targeted Next-Generation Sequencing in Diagnosis of Pulmonary Tuberculosis in Bronchoalveolar Lavage Fluid

Zhen Yang,* Qian Liu,* Yi Hu, Shuang Geng, Ji-Xiang Ni Department of Respiratory and Critical Care Medicine, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, People’s ...
Geng S, Yang Z, Hu Y, Ni JX, Liu Q
core  

High-resolution mapping of evolutionary trajectories in a phage

, 2009
Experimental evolution in rapidly reproducing viruses offers a robust means to infer substitution trajectories during evolution. But with conventional approaches, this inference is limited by how many individual genotypes can be sampled from the ...
Nekrutenko, A, Dickins, B
core   +1 more source

Standardized Protocol for Isolation and Cryopreservation of Cultivable Endophytes From Fresh and Dried Citrus aurantium Peels

Food Safety and Health, EarlyView.
A protocol for converting Citrus aurantium peel—from both fresh fruit and dried Zhike—into a curated endophytic microbial library through standardized surface sterilization, tissue homogenization, and multi‐medium culturing, followed by 16S/ITS‐based identification and −80°C cryopreservation, enabling reproducible exploration of citrus peel microbiota ...
Hujing Cao, Xinlin Huang, Han Yang, Manxi Wu, Jingping Cao, Chongde Sun, Yue Wang   +6 more
wiley   +1 more source

Seasonal drivers of plant–soil–microbial community structure and functions in Western Himalayan grasslands, Pakistan

Grassland Research, EarlyView.
Plant species richness was greater in summer in both alpine and subalpine grassland ecosystems. Soil moisture demonstrates significant positive correlations with soil organic carbon and total nitrogen across seasonal periods. Microbial α‐diversity peaks during summer but maintains functional stability across seasons.
Huma Ali, Muhammad Rafiq, Allan Degen, Ben Sparrow, Zhanhuan Shang   +4 more
wiley   +1 more source

Diagnostic efficacy of metagenomic next-generation sequencing for spinal infections

陆军军医大学学报
Objective To comparatively evaluate the diagnostic value of metagenomic next-generation sequencing (mNGS) versus conventional microbial culture in spinal infections.
LIU Shuang, HE Jinyue, CHEN Hui
doaj   +1 more source

Clinical Application of Metagenomic Next-Generation Sequencing in Sepsis Patients with Early Antibiotic Treatment

Yongru Chen,1 Chongyue Chen,1 Wei Chen,2,3 Wei Gai,4 Yafeng Zheng,4 Yuxin Guo,4 Zhaoning Wang,1 Yongsong Chen,1 Zhiming Cai1,5,6 1The First Affiliated Hospital of Shantou University, Shantou, Guangdong, People’s Republic of China; 2Department of Urology,
Gai W, Zheng Y, Guo Y, Chen W, Cai Z, Chen Y, Chen C, Wang Z   +7 more
core  

Use of Metagenomic Next-Generation Sequencing to Identify Pathogens Involved in Central Nervous System Infections

Liying Zhan,1,* Zhihua Lv,2,* Yunjing Zhang,3,* Jingdi Chen,4 Lu Wang,1 Raojuan Huang,5 Yaqi Sun,5 Wei Wu1 1Department of Critical Care Medicine, Renmin Hospital of Wuhan University, Wuhan, Hubei, People’s Republic of China; 2Department of ...
Lv Z, Chen J, Zhang Y, Zhan L, Wu W, Huang R, Sun Y, Wang L   +7 more
core  

Antibiotic Remodeling of the Gut Microbiota Impairs Host Resistance to Secondary Infection

iMetaMed, EarlyView.
Gut microbial dynamics induced by four antibiotics, revealed distinct taxonomic and functional shifts. Vancomycin‐perturbed bacteriomes were proven to promote pathogen resistance to antibiotic treatment. Metronidazole‐treated mice showed pronounced Salmonella translocation to hepatic tissue, and altered Enterococcus avium populations modulated host ...
Miaoyu Li, Ying Chen, Xi Chen, Weili Lu, Jie Liao, Jiaqi Wang, Qiwei Liu, Yanzi Chang, Zeping Xu, Yanye Tu, Yang Zhou, Jing Xue, Binbin Wang, Kang Chen, Keyun Cheng, Chunlai Zeng, Hong Li, Qing Shen   +17 more
wiley   +1 more source

Metagenomic Next-Generation Sequencing Unravels Talaromyces Marneffei-Induced Bone Destruction in an HIV-Negative Woman: A Case Report with Fatal Outcome

Infection and Drug Resistance
Jiayi Chen,* Zixiang Pang,* Chengyu Qin, Zuhai Huang, Yuanming Chen Department of Spinal Orthopedics, The Second Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, People’s Republic of China*These authors ...
Chen J, Pang Z, Qin C, Huang Z, Chen Y
doaj  

bacNeo: A Computational Toolkit for Identifying Bacteria‐Derived Neoantigens in Human Cancers

iMetaMed, EarlyView.
We present bacterial neoantigen (bacNeo), a multi‐omics‐based computational software to classify bacterial components (BACC), type bacteria‐bound HLA alleles (BACH), and prioritize bacterial peptides as neoantigens (BACP). Bacterial neoantigen potential is quantified by score for peptide antigenicity recognition & kinetics (SPARK), enabling ...
Yunzhe Wang, Li Yuan, Katie W. Gu, Xiangdong Cheng, Bo Wen, Zhao Zhang   +5 more
wiley   +1 more source