Results 121 to 130 of about 30,985 (257)

Integrated one‐pot CRISPR‐Cas detection: Addressing strategies and future prospects

Interdisciplinary Medicine, EarlyView.
Integrated one‐pot CRISPR‐Cas detection significantly simplifies workflows, reduces cross‐contamination risks, and shortens turnaround times, making it highly suitable for point‐of‐care testing (POCT) and resource‐limited settings. Key challenges include system compatibility issues between Cas proteins and isothermal amplification, limited multiplexing
Jian Zhou, Xuemei Ren, Yingjie Ren, Ruiping Wu, Ruiman Geng, Le Chen, Zhuo Li   +6 more
wiley   +1 more source

Impact of Metagenomic Next-Generation Sequencing on Antibiotic Management in Pediatric Patients. [PDF]

Medicina (Kaunas)
Gonzalez A, Argotsinger J, Oram RJ, Miller JL.   +3 more
europepmc   +1 more source

Enhanced CRISPR‐cas systems for genome editing and molecular diagnostics

Interdisciplinary Medicine, EarlyView.
This review systematically summarizes the major optimization strategies for CRISPR‐Cas systems, focusing on the engineering of Cas proteins, guide RNAs, reporter probes, and the incorporation of chemical additives. The aim is to enhance editing precision and diagnostic capability, thereby expanding the CRISPR system's potential for broader biomedical ...
Sheng Li, Han Li, Bojie Chen, Ling Jiang, Keyu Long, Jiaqi Lang, Xingxiang Pu, Erhu Xiong   +7 more
wiley   +1 more source

Metagenomic next-generation sequencing to diagnose atypical severe scrub typhus

Journal of Microbiology, Immunology and Infection, 2022
Zuan Zhan, Chun-Fan Li, Jian Liu, Chun-Shui Cao, Liang Huang   +4 more
doaj   +1 more source

The complexity of invasive fungal diseases in the intensive care unit: evaluation of metagenomic next-generation sequencing. [PDF]

Front Cell Infect Microbiol
Wang R, Yang H, Zhang C, Zi Neng X.
europepmc   +1 more source

Metagenomic Next-Generation Sequencing in Central Nervous System Angiostrongyliasis

Indian Pediatrics, 2022
Liao, Qiong, Zhu, Yu, Wen, Yang
openaire   +2 more sources

A Comprehensive Study of Bidirectional Interactions Between the Human Microbiome and Blood Malignancies and Hematologic Conditions: Focus on Novel Therapeutic Strategies

Journal of Clinical Laboratory Analysis, EarlyView.
Microbiota‐targeted therapeutic strategies in hematological disorders. aHSCT: Transplanted immune cells from the donor may attack the recipient's tissues, especially when damage to the intestinal epithelium disrupts the gut microbiota, contributing to GVHD. Maintaining a balanced gut microbiota that supports immune regulation helps reduce GVHD risk and
Alireza Molajafari, Hadi Sedigh Ebrahim‐Saraie, Majid Taati Moghadam, Meysam Hasannejad‐Bibalan   +3 more
wiley   +1 more source

Rapid diagnosis of Lemierre's syndrome by metagenomic next-generation sequencing: a case report. [PDF]

Front Med (Lausanne)
Wang Y, Zhang X.
europepmc   +1 more source

Organ‐Specific Human Microbiomes and Dysbiosis: Mechanistic Links to Disease and Emerging Therapeutic Strategies

Journal of Clinical Laboratory Analysis, EarlyView.
Schematic overview of the human microbiome and major microbiota‐derived metabolites across body sites, highlighting the gut–brain, gut–heart, and gut–kidney axes in host physiology and disease. ABSTRACT Background The human microbiome is a dynamic and diverse community of microorganisms that affects susceptibility to illness and promotes wellness ...
Awadh Alanazi
wiley   +1 more source

Betaine‐, xylitol‐, and extra virgin olive oil–based toothpaste modulates inflammation and oral nitric oxide pathway: Clinical trial in gingivitis

Journal of Periodontology, EarlyView.
Abstract Background Gingivitis is a prevalent and reversible inflammatory condition driven by microbial dysbiosis. Beyond plaque control, interest has grown in oral hygiene formulations capable of modulating inflammation while preserving key microbial functions. Extra virgin olive oil (EVOO), together with xylitol and betaine, has demonstrated clinical
Francisco Mesa, Alejandro Rodríguez‐Agurto, Marco Bonilla, Debora Rodríguez‐Vilaboa, Beatriz Rodríguez‐Vilaboa, Manuel Bravo   +5 more
wiley   +1 more source