Results 21 to 30 of about 1,873,698 (292)

Repurposing the Damage Repair Protein Methyl Guanine Methyl Transferase as a Ligand Inducible Fusion Degron [PDF]

ACS Chemical Biology, 2022
We successfully repurpose the DNA repair protein methylguanine methyltransferase (MGMT) as an inducible degron for protein fusions. MGMT is a suicide protein that removes alkyl groups from the O6 position of guanine (O6G) and is thereafter quickly degraded by the ubiquitin proteasome pathway (UPP). Starting with MGMT pseudosubstrates (benzylguanine and
Murawska, Gosia M., Vogel, Caspar, Jan, Max, Lu, Xinyan, Schild, Matthias, Slabicki, Mikolaj, Zou, Charles, Zhanybekova, Saule, Manojkumar, Manisha, Petzold, Georg, Kaiser, Peter, Thomä, Nicolas, Ebert, Benjamin, Gillingham, Dennis   +13 more
openaire   +4 more sources

O-methylguanine-DNA methyltransferase (MGMT) mRNA expression predicts outcome in malignant glioma independent of MGMT promoter methylation [PDF]

, 2011
Background: We analyzed prospectively whether MGMT (O(6)-methylguanine-DNA methyltransferase) mRNA expression gains prognostic/predictive impact independent of MGMT promoter methylation in malignant glioma patients undergoing radiotherapy with ...
Kreth Simone, Friedrich W. Kreth, Hans A Kretzschmar, Lutz Juergen, Ludwig C. Hinske, Friedrich W Kreth, Ledderose Carola, Kretzschmar, Hans A., Kreth, Friedrich-Wilhelm, Juergen Lutz, Eigenbrod, Sabina, Kreth, Simone, Tonn, Joerg C., Thon Niklas, Thon, Niklas, Eigenbrod Sabina, Joerg C Tonn, Hinske, Ludwig C., Egensperger Rupert, Kretzschmar Hans A., Carola Ledderose, Rupert Egensperger, Kreth Friedrich W., Joerg C. Tonn, Lutz, Juergen, Niklas Thon, Hans A. Kretzschmar, Kreth, Friedrich W., Egensperger, Rupert, Hinske Ludwig C., Hinske, Ludwig Christian, Ludwig C Hinske, Sabina Eigenbrod, Tonn Joerg C., Ledderose, Carola, Simone Kreth   +35 more
core   +1 more source

Loss of histone H3 methylation at lysine 4 triggers apoptosis in Saccharomyces cerevisiae. [PDF]

PLoS Genetics, 2014
Monoubiquitination of histone H2B lysine 123 regulates methylation of histone H3 lysine 4 (H3K4) and 79 (H3K79) and the lack of H2B ubiquitination in Saccharomyces cerevisiae coincides with metacaspase-dependent apoptosis.
David Walter, Anja Matter, Birthe Fahrenkrog   +2 more
doaj   +1 more source

Repair of Methylation Damage in DNA and RNA by Mammalian AlkB Homologues [PDF]

Journal of Biological Chemistry, 2005
Human and Escherichia coli derivatives of AlkB enzymes remove methyl groups from 1-methyladenine and 3-methylcytosine in nucleic acids via an oxidative mechanism that releases the methyl group as formaldehyde. In this report, we demonstrate that the mouse homologues of the alpha-ketoglutarate Fe(II) oxygen-dependent enzymes mAbh2 and Abh3 have ...
Lee, Dong-Hyun, Jin, Seung-Gi, Cai, Sheng, Chen, Yuan, Pfeifer, Gerd P, O'Connor, Timothy R   +5 more
openaire   +4 more sources

Decreased KAT5 Expression Impairs DNA Repair and Induces Altered DNA Methylation in Kidney Podocytes

Cell Reports, 2019
Summary: Altered DNA methylation plays an important role in the onset and progression of kidney disease. However, little is known about how the changes arise in disease states.
Akihito Hishikawa, Kaori Hayashi, Takaya Abe, Mari Kaneko, Hideki Yokoi, Tatsuhiko Azegami, Mari Nakamura, Norifumi Yoshimoto, Takeshi Kanda, Yusuke Sakamaki, Hiroshi Itoh   +10 more
doaj   +1 more source

Epigenetic regulation during fetal femur development: DNA methylation matters [PDF]

, 2013
Epigenetic modifications are heritable changes in gene expression without changes in DNA sequence. DNA methylation has been implicated in the control of several cellular processes including differentiation, gene regulation, development, genomic ...
Oreffo Richard O. C., Oreffo, Richard O. C., Antonio Gonzalez, González Martínez-Pedrayo, Antonio, Richard O C Oreffo, de Andrés María C., Roach Helmtrud I., Wilson, David I., Kingham, Emmajayne, Kingham Emmajayne, Helmtrud I Roach, Oreffo, Richard O C, Emmajayne Kingham, Kei Imagawa, María C de Andrés, Roach, Helmtrud I, Wilson David I., de Andrés, María C., Imagawa, Kei, Roach, Helmtrud I., De Andrés González, Mª Carmen, Imagawa Kei, Wilson, David I, Neves, Nuno M., David I Wilson, Gonzalez, Antonio, Gonzalez Antonio   +26 more
core   +1 more source

Dynamic changes of DNA methylation induced by benzo(a)pyrene in cancer

Genes and Environment, 2023
Benzo(a)pyrene (BaP), the earliest and most significant carcinogen among polycyclic aromatic hydrocarbons (PAHs), has been found in foods, tobacco smoke, and automobiles exhaust, etc.
Huizeng Wang, Bingchun Liu, Hong Chen, Peixin Xu, Huiting Xue, Jianlong Yuan   +5 more
doaj   +1 more source

Transcription influences repair-induced DNA methylation [PDF]

, 2008
This work is aimed at the dissection of the molecular mechanism(s) linking DNA damage and gene silencing. To this end, we have developed a genetic system that allows a rapid assessment of homologous-directed repair (HR) of an unique DNA double strand ...
Morano, Annalisa
core   +1 more source

DNA methylation in follicular lymphoma [PDF]

, 2010
PhDFollicular Lymphoma (FL) is a common B cell Non-Hodgkin Lymphoma with a median survival of 8-10 years. Patients frequently undergo transformation to a more aggressive lymphoma and this is associated with drastically reduced survival.
Ó Riain, Ciarán Liam
core   +2 more sources

Update on DNA methylation in UV radiation-related skin diseases

Pifu-xingbing zhenliaoxue zazhi, 2023
DNA methylation is a common epigenetic phenomenon that changes genetic expression without altering the DNA sequence. With deepening research on DNA methylation, more and more variable positions of methylation have been identified in a variety of diseases(
Wenting CHEN, Xinni ZHONG, Wei LI
doaj   +1 more source