Application of image-recognition techniques to automated micronucleus detection in the in vitro micronucleus assay [PDF]
Genes and EnvironmentBackground An in vitro micronucleus assay is a standard genotoxicity test. Although the technique and interpretation of the results are simple, manual counting of the total and micronucleus-containing cells in a microscopic field is tedious.
Hiromi Yoda +4 more
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Cytokinesis-block micronucleus assay of celecoxib and celecoxib derivatives [PDF]
Toxicology Reports, 2020 Celecoxib is used widely for the acute treatment of pain and for pain relief in various diseases. Furthermore, it shows potential in chemoprevention, although chronic treatment with celecoxib could lead to adverse effects like cardiovascular events.
Hauke Reimann +3 more
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In vitro micronucleus assay: Method for assessment of nanomaterials using cytochalasin B [PDF]
Frontiers in Toxicology, 2023 The in vitro micronucleus (MNvit) assay is used to evaluate the aneugenic and clastogenic potential of a test material based upon its ability to induce micronuclei in the cells.
Christopher S. Farabaugh +3 more
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The effect of aging on the repeated-dose liver micronucleus assay [PDF]
Genes and Environment, 2021 Background The liver micronucleus (MN) assay is an effective and important in vivo test for detecting genotoxic compounds. In particular, the repeated-dose liver MN (RDLMN) assay which greatly facilitates incorporation of the liver MN assay into the ...
Miyuki Shigano +2 more
doaj +2 more sources
The effect of aging on the repeated-dose liver micronucleus assay using diethylnitrosamine [PDF]
Genes and Environment, 2022 Background The repeated-dose liver micronucleus (RDLMN) assay has been well-developed and applied because of its simplicity and the ease of integration into general toxicity studies which is the preferred method from the 3R’s point of view. In this assay,
Kensuke Satomoto +6 more
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Confirmation of Di(2-ethylhexyl) phthalate-induced micronuclei by repeated dose liver micronucleus assay: focus on evaluation of liver micronucleus assay in young rats [PDF]
Genes and EnvironmentBackground Di(2-ethylhexyl) phthalate (DEHP) is a plasticizer commonly used in a wide variety of products, including medical devices. It is rapidly metabolized in the liver into various metabolites upon absorption through oral ingestion, dermal ...
Miyuki Shigano +5 more
doaj +2 more sources
Potentials of cytokinesis blocked micronucleus assay in radiation triage and biological dosimetry [PDF]
Journal of Genetic Engineering and BiotechnologyThe measurement of micronucleus (MN) in the cytokinesis-block arrested binucleated cells has been extensively used as a biomarker in many radiation biology applications in specific biodosimetry.
G. Tamizh Selvan, P. Venkatachalam
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Micronucleus assay: Pitfalls and challenges [PDF]
Journal of Cytology, 2020 Noushin Jalayer Naderi
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Micronucleus Assay: The State of Art, and Future Directions [PDF]
International Journal of Molecular Sciences, 2020 Iwona Buraczewska, Marcin Kruszewski
exaly +2 more sources
Genotoxicity of Di (2-ethyl hexyl) phthalate
Zhongguo shipin weisheng zazhi, 2022 ObjectiveTo assess the genotoxicity of Di（2-ethylhexyl） phthalate （DEHP）.MethodsA series of guideline-compliant assays were performed to evaluate mutagenicity， chromosomal or DNA damage of DEHP， including Ames test， in vitro mammalian chromosome ...
LI Juntao +9 more
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